Wheat microsatellite WMC 25/06/01
wmc56(1f7)
Plate reference : 1f7
WMC locus number : WMC56 Length of sequence : 612
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)12 109 to 132, (CT)10 89 to 108. Bases 49 to 68 show homology to 12c10.1 bases 43 to 62 (20 in longest run, maxScore = 32).
PCR Comments : 61C anneal, product;
Forward primer details :
TTcgTccccATcccAgAcTAAc at 29 length 22 bases, Tm 66
Reverse primer details :
AgAgATATcgATTcgggggTgc at 197 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 190 bases.
Length of sequence : 612 Consensus sequence is :
..
ACTACATTAC ATTTGTTGTG GTCTAATTTT CGTCCCCATC CCAGACTAAC
ATTATTCATT TGTTTACCCA CAGACAAACT CCCCCACCCT CTCTCTCTCT
CTCTCTCTCA CACACACACA CACACACACA CAACTCCTGC TCCACACCCC
TTCCCGACTA CTGTCTCTAT CTCACACACA CAAAACTCTT GCTTTCGCAC
CCCCGAATCG ATATCTCTCA TAAACATTTA TTGACTAGCC TCTAGATAGG
TTTATACACT CGCACACTCG TGCTTCCGCT TCGCATACAT GGCTCTCTCC
CGCTCCTTGT ATCTATGTAG ATTTTTCTTC CCTTCTTGAT ACACGCCCTC
TGATCGTGCA CACACACACT ATTGCATCAT TTTAAGCTGA TATCTCTAGC
ACACACGCTC TTTGTGTCTT TTCACACATG AATCCGTCCT CTTCCTCTCT
CCCTCTCTCT CACACACACA TGGGCATTTT GCCACAACTA CAAGATGCCC
ATGCATTGCA TGGAACATCA AGATGCATTT GTATGAGTAG TTTACTCAGA
AGTAAACATA TAGAACACGA GCATGGTCAT CATGATGAAA TGAAGACAAG
CACAGAGTTA GT
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wmc57(1g7)
Plate reference : 1g7
WMC locus number : WMC57 Length of sequence : 369
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)15 158 to 187. May be dominant marker.
PCR Comments : 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AAcTATgTgcTAcccAAcAgAc at 135 length 22 bases, Tm 58
Reverse primer details :
gAcTAcgATgAcAAccccAAAT at 242 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 129 bases.
Length of sequence : 369 Consensus sequence is :
..
ACTACCATGA TTTTAAATCG CACACATGAT TTCTAGAACT GAAGAAGCTA
TCTCATATTG TAGATGCAAA GCATAGCAGA TTAACAACTC CAAGAAGGAA
ACAACAGATT TCATTTAAGA ACGTGCTAGG TTTTAACTAT GTGCTACCCA
ACAGACCcAC ACACACACAC ACACACACAC ACACACAAAG GTAGATAATG
TTATTCGGTT CCACTGCATG CTCCTCGATG GCATCCGAGG CATTTGGGGT
TGTCATCGTA GTCGGGTGAG TCCGTCTCGA TACATGGCAC AGGACTAAAT
CATCCTACAT CGATTAAGCG ACGCATTAAA CAATGGGAAT ACATCCCAAT
AGAATGATTA TGGGTTAGT
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wmc(1h7)
Plate reference : 1h7
WMC locus number : WMC Length of sequence : 305
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :
Length of sequence : 305 Consensus sequence is :
..
ACTAGTCCAC TTTCCTACCC CTGCCGCCGC CGCCGCCTCC GCCTCTCCCC
ACCAGCCACC GGGTCCAACC ACCAAGTCGC GCACGATGGC GACCTCCTCG
CGTCTCGGCG GTGCCCGCCT CCCCTCCGGA TCCACATCCT CATCATTGCC
GGGGCCGTGA GCGGCTACCA CTTGCTCAAG GTCGACGGCT ACTCGCGCAC
CAAGGAGCTa CCCAATGGCG AGTGGATCGA CTCTTGCCCG TTCCAGGTGG
GAGGCCGCAC ATGGCATcTT tCGTTACTAT CCAACGGGGA CAAGTCCGAG
TTAGT
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wmc58(1a8)
Plate reference : 1a8
WMC locus number : WMC58 Length of sequence : 269
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GCC)6 175 to 192
PCR Comments : 61C anneal, product;
Forward primer details :
ATcggcggTgAAgcTgATAAgT at 32 length 22 bases, Tm 66
Reverse primer details :
TgAAAcAggggAATcAgAgggT at 247 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 237 bases.
Length of sequence : 269 Consensus sequence is :
..
ACTACCTGAG GCTCACCGCT GAGGAAAACG AATCGGCGGT GAAGCTGATA
AGTGCTTATG GTGGTGCCGC GTTGATCTCA GGAACCTGCT CCCTGCTAGG
TGCTCAGGGC GGCGCCGTCG TCTCTGCAAC AGCAACCCCG CGTcTtGTGC
CGCCGTGATA ACCACAGCAT CCCCGCCGCC GCCGCCGCCG CCTTCTTCAA
CTCACTGTTC AAAGGTATCT GAACTTCTGA AGTCTGAACA TCGAAAACCC
TCTGATTCCC CTGTTTCAT
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wmc(1b8)
Plate reference : 1b8
WMC locus number : WMC Length of sequence : 0
Last updated : 15 Nov 1996 at 15:24
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No insert.Originally vector sequences showed cross hybridisation to 1B8, 1A7, 1F8, 1F9, 1G11
PCR Comments :
Length of sequence : 0 Consensus sequence is :
..
GGATCCTCTA gAGTCGACCT GCAGGCATGC AAGCTT
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wmc(1c8)
Plate reference : 1c8
WMC locus number : WMC Length of sequence : 179
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :
Length of sequence : 179 Consensus sequence is :
..
ACTAACACAG ATCCTATATC GTAAGTTTAA AACATACGAG TAATTCGTGG
ATGGATGCGT TAACAAGGCA GCAATTGAAG GAGAAGCAAG GGTTTTGGTC
TGACGGAGGC GCTCCCGTAG TATATATACC TGCATCTGAG AGGACTTGGA
TGTTAACTCC GCCGCCGCCG CCGCCTAGT
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wmc(1d8)
Plate reference : 1d8
WMC locus number : WMC Length of sequence : 0
Last updated : 15 Nov 1996 at 15:40
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No insert. Originally vector sequence caused cross hybridisation to vector in 1a9, 1d8, 1b10.
PCR Comments :
Length of sequence : 0 Consensus sequence is :
..
ACTGCAGGCA TGCaAGCTT
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wmc59(1e8)
Plate reference : 1e8
WMC locus number : WMC59 Length of sequence : 330
Last updated : Tue, Dec 9, 1997 at 3:36 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)19 103 to 140. Bands a little weak. Barley - mapped 1HL tip Lina x Canada Park (R. Waugh et al) - at 55C anneal.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TcATTcgTTgcAgATAcAccAc at 74 length 22 bases, Tm 63
Reverse primer details :
TcAATgcccTTgTTTcTgAccT at 249 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 197 bases.
Length of sequence : 330 Consensus sequence is :
..
ACTAACCTCT GCCATTCGAT GAGAACCTTC ATTGCAGTCT CCTTTGGATC
ATACACGGGA GAGAAGAAAA AGTTCATTCG TTGCAGATAC ACCACAATCC
GGCACACACA CACACACACA CACACACACA CACACACACA ACACACACAT
ATATCTTTGA TAATTATGAA GATGTTCAAG AGATGCCCAT AATCACATTT
GCATGCACAC CCGGTTCATA GAAATAGCAA AGAGATAAGT CTTATAAAAG
GTCAGAAACA AGGGCATTGA TTGAGAGTGA GAGAGACCGT ATCTGAAACA
CAACAGCTAT GATACAGAAC CCATGCTAGT
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wmc(1f8)
Plate reference : 1f8
WMC locus number : WMC Length of sequence : 0
Last updated : 15 Nov 1996 at 15:32
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No Insert. Original sequence was showing vectore homology to 1B8, 1A7, 1F8, 1F9, 1G11
PCR Comments :
Length of sequence : 0 Consensus sequence is :
..
AGCGCGCCTC AAGGGATGcT CCagAGTCGA AcTGCAGGCA TGCTAGCTT
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wmc(1g8)
Plate reference : 1g8
WMC locus number : WMC Length of sequence :
Last updated : 14 Oct 1996 at 13:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No sequence obtained
PCR Comments :
No sequence available for this clone
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wmc(1h8)
Plate reference : 1h8
WMC locus number : WMC Length of sequence : 272
Last updated : Tue, Aug 5, 1997 at 5:52 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GA)8 183 to 198, microsatellites too close to end. Member of 1g1 family.
PCR Comments :
Length of sequence : 272 Consensus sequence is :
..
ACTAACAGAT GATAAGTTTG CTGACTAATA AAGTAAAGTG GAATTTGTCC
ATGTTATGCA AGTAAATTAA GTGATTGTTT ATCATACGGG TAAGGTTGGA
TGAAGAGTGG TAGGAAAAAA TGCTCCGCCT AGAACATGTG GTGTGTGATG
GAGTCTTATG TGTGTGTGGG GTGTGTGTGT GTGAGAGAGA GAGAGAGAAA
GAGAGAGAGA AGATGGAGTC TTAGTTTGTG TGTGTGTGAA AGAGAGAGAT
GGAGTCTTAG TGTGTGTGGA GT
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wmc(1a9)
Plate reference : 1a9
WMC locus number : WMC Length of sequence : 172
Last updated : 15 Nov 1996 at 15:38
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : Trimmed to Rsa1 site at 3' end. originally vector sequence beyond this caused cross hybridisation to 1a9, 1d8, 1b10. No substantial microsatellite.
PCR Comments :
Length of sequence : 172 Consensus sequence is :
..
ACTAGCGGGG aCaATGGTGA AATGGACCAG CGGTTCAAGG CGGCGGCGCG
CGGCGAACCG AGGCGAGCAC GTGGAgTTGT GAATATTGTC CAGACGCTGA
TAGTGGATAA ATACGTCTCC TCTAACTTTT CCATGCAATA CCATGATGAC
TGTCGGGTAA CACCCATGGT AC
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wmc(1b9)
Plate reference : 1b9
WMC locus number : WMC Length of sequence : 400
Last updated : Wed, Aug 6, 1997 at 9:26 am
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite. Contains some sequence related to 1e12.
PCR Comments :
Length of sequence : 400 Consensus sequence is :
..
ACTAACATAT GGGCCTGCAA TAATTGAGTT ACAGTAATAC TTAGTGTAAA
CAGCCGCTCT GCGGATATCC ACAAAAAATA AACAGCCGCT CTGCAGATAG
AGATGGCGGC GGCGGCGGCC GAGGCGGCGG CTACCGGATA GGGGTCAATC
CTCGGTCGGT GGGCGGTGGC GGTGTCATAG GAGTTCAATC CTCGGTCTGC
GGCAGGAGAT GGAGGAGCTC AACCATCGAG GTCGGCGGCG GCGTGATTGG
AGCACATCCA TCGCGGTCGA TGGCGGGATA TGGAGGTTGA ACTTCAGGCG
GTAGCCGCAC TAAGCTTTGC TCCTCGACCT TGCTATCTCA GTGTGCCATT
CATCGCGCAT CTCTGTCTGC TGGGTGGAGG CCGCCACACG GCTAACTAGT
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wmc60(1c9)
Plate reference : 1c9
WMC locus number : WMC60 Length of sequence : 373
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)28 146 to 201. (2 polymorphic loci), bands a little weak.
PCR Comments : 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
TcATAcAcAAAAgATTcAggAT at 59 length 22 bases, Tm 56
Reverse primer details :
TggAgATAcAAAgAAccATAcT at 214 length 22 bases, Tm 55
Expected size of amplified fragment (Chinese Spring=) 177 bases.
Length of sequence : 373 Consensus sequence is :
..
ACTAACACAC CTCTATAGAG TCCAAATTTT TGCAGGATGT TGGAAAACTT
GTTGGTGTTC ATACACAAAA GATTCAGGAT ATTTTAACTT TTTACTATTT
TCAGAATTTA CTGTTCATGC GGGTTGTCAC TTACAGACCT TTATACACAC
ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC
ATATATATAG TATAGTATGG TTCTTTGTAT CTCCATTTAT TCTTTTAGTT
CTTACATCTA TTTTAACGTT TGATGTAATT CACTACATTG TTATTATCCA
TCAAAGCAAT ACTAAAAGAC ACAATAGTAA TGAATTAAAA TAACATGTCT
CATATTTCTA TTTATATTCT AGT
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wmc61(1d9)
Plate reference : 1d9
WMC locus number : WMC61 Length of sequence : 355
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)9 85 to 102, (CT)8 103 to 118. Duplicate of 7D11.
PCR Comments : 51C anneal, product;
Forward primer details :
TcTgTTATcATTgccTcAgTcT at 46 length 22 bases, Tm 58
Reverse primer details :
ATcTTTTTcAcgTcTcgTgTTc at 191 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 167 bases.
Length of sequence : 355 Consensus sequence is :
..
ACTAACATAT CTCTCTCTAT CCTCCACACA CATAGACACA AAGAATCTGT
TATCATTGCC TCAGTCTCTA ACATATCACA CACGCACACA CACACACACA
CACTCTCTCT CTCTCTCTCG CAAACACGCT CAACAAGGGT TCCCCGTGAA
TAACTTACCG TCTATTCATC AACAGTCGTG GCAGTATGGC GAACACGAGA
CGTGAAAAAG ATAAATCTAC ATGTGCTTAG ACCACCTAGT ATGACTACTA
GGACTAGAGC AAGCCAAAAA GCGCGCCGCC TCACCCCCTC CTTATAGGCG
ACGGGAAACC TTTGTTTTAC AAAGTTGAGA AGTCATTGTG CTAAGGCCTC
CTAGT
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wmc(1e9)
Plate reference : 1e9
WMC locus number : WMC Length of sequence : 346
Last updated : 8 Oct 1996 at 12:45
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CT)17 15 to 48, flanking sequence too short to make primers
PCR Comments :
Length of sequence : 346 Consensus sequence is :
..
ACTAGTTCAG TCACCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTAC
TTTCCTCCAA CTGATCGACC TGATTTCGAC CCCAGCTTGT TTCTGGGTTG
GCTGATTCCC GATCCCCTCT ACCCTGGGGG TGGCTAGTCC ATTTATAGAG
GCCCTGGTCC TCTTCCCAAA AATTGAGCGG GAAGGGAGCC AACAATGGCG
GGCTAATTTG AGGGGGACAA CAAGTATCAG CTATCCTGAC AAAAGAAGTC
TTCGCCTGTG CAAAGCTCTG GTGATGACGC GTCTTGGGCT CCACGGTGAC
CTCCATCTCG TAGTCCTCCT GGTCTTGGTC TCGTTGCACC GAAATG
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wmc(1f9)
Plate reference : 1f9
WMC locus number : WMC Length of sequence : 200
Last updated : 15 Nov 1996 at 15:31
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : Trimmed to 3' Rsa site. Originall vector sequence caused cross hyb to 1B8, 1A7, 1F8, 1F9, 1G11.
PCR Comments :
Length of sequence : 200 Consensus sequence is :
..
ACTCTGGGAC CGCTGCAGGT TCCGATGTAG AGGCTTCCTC TTCTGGTTCG
CCTACTACCC CGTCTCCTCC CCGATCCCGT CGCCTCCGCC GCCGCCCCGA
CCCACAAATC GCGCTCCCGC ACCGAGTCCT GGGTCTCCAC CTCGCGCCCA
CCGCCGCCGG CACCCGCAAG CCGGGCCAgA AaCCTGAGCC CTGTCTGTAC
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wmc62(1g9)
Plate reference : 1g9
WMC locus number : WMC62 Length of sequence : 212
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GT)11 118 to 139, (GT)11 141 to 162, reverse sequence = 1h9.
PCR Comments : 61C anneal, product;
Forward primer details :
TcAccgcgggAAAATTTAAcAc at 71 length 22 bases, Tm 67
Reverse primer details :
AAcccAcATgATggcTcAcgAc at 172 length 22 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 123 bases.
Length of sequence : 212 Consensus sequence is :
..
ACTAACTAAA ACGTCTTAAT GATCTCTGTT TTTTTTCAAA ATTAAAACGT
CCCTCCTTTG GTAACATATG TCACCGCGGG AAAATTTAAC ACCCATCGTA
GTTGCGATGG ATGCGCGGTG TGTGTGTGTG TGTGTGTGTG GTGTGTGTGT
GTGTGTGTGT GTGCACTACG AGTCGTGAGC CATCATGTGG GTTGGCCATT
TTGTGAGGCT GT
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wmc(1h9)
Plate reference : 1h9
WMC locus number : WMC Length of sequence : 212
Last updated : 8 Oct 1996 at 12:53
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)10 51 to 70, (CA)12 72 to 95, reverse of this sequence = 1g9. Primers given on record for 1g9
PCR Comments :
Expected size of amplified fragment (Chinese Spring=) 123 bases.
Length of sequence : 212 Consensus sequence is :
..
ACTAGCCTCA CAAAATGGCC AACCCACATG ATGGCTCACG ACTCGTAGTG
CACACACACA CACACACACA ACACACACAC ACACACACAC ACACACCGCG
CATCCATCGC AACTACGATG GGTGTTAAAT TTTCCCGCGG GAACATATGT
TACCAAAGGA GGGACGTTTT AATTTTGAAA AAAAACAGAG ATCATTAAGA
CGTTTTAGTT GT
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wmc63(1a10)
Plate reference : 1a10
WMC locus number : WMC63 Length of sequence : 343
Last updated : 30 Sep 1996 at 12:28
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GA)12 142 to 165,
PCR Comments :
Forward primer details :
gTgcTcTggAAAccTTcTAcgA at 23 length 22 bases, Tm 62
Reverse primer details :
cAgTAgTTTAgccTTggTgTgA at 193 length 22 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 192 bases.
Length of sequence : 343 Consensus sequence is :
..
ACTAACAAAC GATGGGTGCT CCGTGCTCTG GAAACCTTCT ACGATCTCCT
TTGTAAGAGT AAATTATTTT GGCAGCGGCA TGAGCTAAGC TGCCACCAAA
AACACGAATC AACTTTTCTT TTGCAGATAA TGCTGAGAGA AGAGAGAGAG
AGAGAGAGAG AGAGAAGTGC ACTTTGGCTT TGTGAAGTGG TGTCACACCA
AGGCTAAACT ACTGTGCAGT GATTATAATA CAAGCCACTA GCACCAGAAT
ATGTCTGTAA ACAAACTAGG ACCAGAATAT GTTATTGCAA ATATAACGCT
TTCTCTTTAC GGAGAAGAAG AGATTAATCA CGAAAGTCTG ACA
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wmc(1b10)
Plate reference : 1b10
WMC locus number : WMC Length of sequence : 28
Last updated : Tue, Aug 5, 1997 at 6:03 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : Originally vector beyond 3' end caused cross hybridisation to 1a9, 1d8, 1b10. Degenerate vector sequence at 3' end removed 5.8.97
PCR Comments :
Length of sequence : 28 Consensus sequence is :
..
TTTTATCGAC GGCAGGCCCA CCTTAAAG
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wmc64(1c10)
Plate reference : 1c10
WMC locus number : WMC64 Length of sequence : 239
Last updated : Wed, Aug 6, 1997 at 9:09 am
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)10 141 to 160. 1st hundred bases are related to 1F11. Froward primer is fro least homologous part of the alignment. Only improvement would be to move it a few bases 5'.
PCR Comments : 61C anneal, smear;
Forward primer details :
AcATAcAATgggATccAgcAgA at 57 length 22 bases, Tm 63
Reverse primer details :
TTTgTgcATgcAAgTgccAgAA at 191 length 22 bases, Tm 69
Expected size of amplified fragment (Chinese Spring=) 156 bases.
Length of sequence : 239 Consensus sequence is :
..
ACTATGTCTC TCACACATAT GCCTAAGGTC AACTCGAGTT TCGGAACCAT
ATGAATACAT ACAATGGGAT CCAGCAGAGC ACCTTTTGTT TTGAGATTTC
GCTCTCTCTC TCACTCTCTC TCTCCCTCTC TCTCTCTCTC CACACACACA
CACACACACA CGCACACACA CATTGTTTCT CGCTTAATTT TTCTGGCACT
TGCATGCACA AATTTTGTTT ATCTTCGTGA TGCTTTAGT
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wmc(1d10)
Plate reference : 1d10
WMC locus number : WMC Length of sequence : 277
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :
Length of sequence : 277 Consensus sequence is :
..
ACTAACAGAA ACCCTACCGT CCACATTTTC CTCCGCCTCT CCGCCGCCGC
CGCCACTTCC CACTACCATG GTGCTATCCA TCCACCCCAG TACGAGTTGC
TCAAATCGAG GATCACCCCA TCCACCTCGA AATATTGGGC GTGGTGCACA
TCCCTCCCGC AAACCCAAAC CAAGACCCAA CGGAAGTCTC CTTCGTGGGG
ACATCCATGC GACTCCGCAT ATGGCCATAG AAGCTGCTGC GTACGAAGCG
CTCGCTCGTC TTCGATTCGC GGTTAGT
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wmc65(1e10)
Plate reference : 1e10
WMC locus number : WMC65 Length of sequence : 398
Last updated : 8 Oct 1996 at 13:23
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GT)22 97 to 140.
PCR Comments :
Forward primer details :
TggATgggAAggAgAATAAgTg at 28 length 22 bases, Tm 62
Reverse primer details :
ATccAAccggAAcTAccgTcAg at 340 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 334 bases.
Length of sequence : 398 Consensus sequence is :
..
ACTAACAGAG GCTTCACCTT CTTGGCGTGG ATGGGAAGGA GAATAAGTGG
GAGTATAGAT GCGGCCTCAT TTCAAATAAA GGACTTGAAC ATGTATGTGT
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT TGTGTGTGTG
TTTGGGTGGG TCTTGAACCA TGCTTATTGA GTTAGAGCAT CTACAACTGT
AAATAGTAAA GGTGCCTCCC AAATGCCGCG TGTGACCTCG AACACTAAAT
TTGCTAACTG TGTTTGATAC AAGGGTTTGG ACCTGATTTA CTTTTTGTGG
GGACAATGGT GGGAGCGGCG TTGGCCATAC CGGGCCAGGC TGACGGTAGT
TCCGGTTGGA TCCGAGCCTC CTTATATCCA CCCCACATAT GAGCTAGT
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wmc(1f10)
Plate reference : 1f10
WMC locus number : WMC Length of sequence :
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No sequence available
PCR Comments :
No sequence available for this clone
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wmc(1g10)
Plate reference : 1g10
WMC locus number : WMC Length of sequence :
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : no sequence available
PCR Comments :
No sequence available for this clone
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wmc(1h10)
Plate reference : 1h10
WMC locus number : WMC Length of sequence : 377
Last updated : Mar 30 mai 2000 at 9:54
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GA)9 47 to 64, (GA)13 286 to 311, sequence has multiple internal duplications. Too difficult to design primers. Part of sequence matches 9b6. Bases 81 to 120 show homology to TW3c09.2 bases 3 to 42 (40 in longest run, maxScore = 27). Bases 143 to 205 show homology to TW3c10 bases 169 to 231 (complement) (63 in longest run, maxScore = 28). Duplicate of TW3c09.2, 1h10 has priority.
PCR Comments :
Length of sequence : 377 Consensus sequence is :
..
ACTAGGTTTG TGTGTTAGAT GCGTGTGACA GAAATGAAGA TTATCAGAGA
GAGAGAGAGA GAGATGGAGG ATAGGGAGAG GGACCAACAA GGTTTGTGTG
TTGGATGCGT GCGACAGAAC TGAAGATTAT CAGAGAGAGA AGAGAGAGAG
AGGGACCAAC AAGGTTTGTG TGTTGGATGC GTGCGACAAA ACTGAAGATT
GTCAGAGAGA AGAGAGAGAT GGAGAGAGAG AGAGGGACCA ACAAGGTTTG
TGTGTTGGAT GCGTGCGACA GAACTGAAGA TATCAGAGAG AGAGAGAGAG
AGAGAGAGAG ATGGAGGGAG AGGGAGAGGG ACCAACAAGG TTTGTGTTTT
CACGCGCCTC GAGGGATCCT CTAGAGT
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wmc66(1a11)
Plate reference : 1a11
WMC locus number : WMC66 Length of sequence : 513
Last updated : 8 Oct 1996 at 15:42
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)11 24 to 45, (CT)7T(CT)9 196 to 229, only possible to design primers to second microsatellite
PCR Comments :
Forward primer details :
ccTATcTAgTAggccTcTcgcA at 154 length 22 bases, Tm 62
Reverse primer details :
cccTTcgAATTggTTgTTgTgg at 313 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 181 bases.
Length of sequence : 513 Consensus sequence is :
..
ACTAGCGATG TCTGCCTACC ATACACACAC ACACACACAC ACACACTTTC
CATCTCCTCC TTTCACTGTC ATGCCTCATG TCTCTCATAT GGTCCCACAC
ACGTGTAAAC TCTCGTCCCT ATTTTCATGG ATTTCACAAT GCACACTCCT
CCCCCTATCT AGTAGGCCTC TCGCACCACC TCCTAGCTCC ATCTATCTCT
CTCTCTCTCT TCTCTCTCTC TCTCTCTCTC GATCAGCCCC CTGGCTATGG
TGGTGGTTAG CCCTATTTAT AGAGGCCCGG TCCTCTCCCC AAATATTAGG
CGGGAAGGGA TCCCACAACA ACCAATTCGA AGGGGGACAT CGAGTATAAG
TATCCTGACA AAAGGTGGTC TTCGCCTGCC AAAGGTTCTG GTGGTGACGC
CGTCGTGGGC TCCGTGGTGA CTCCATCTTG CCGTCCTGCT GGTCTTGGTC
TCGTTGCACA GATATGGAAA CCTTTGCCTG ATGCCTCGGG CTTCTTGTCT
GCGCCTGCCT CTT
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wmc(1b11)
Plate reference : 1b11
WMC locus number : WMC Length of sequence : 522
Last updated : Lun 5 juil 1999 at 17:23
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite. Weak homology to 13F1.2 (ie. second insert in 13F1). Bases 82 to 101 show homology to 26c8 bases 250 to 269 (complement) (20 in longest run, maxScore = 55).
PCR Comments :
Length of sequence : 522 Consensus sequence is :
..
ACTACTGCCG ACAGAAAAAT CTCCGGCTTA CCGTTCGTGT GAAGGCCATA
ACCCGGGGTG ACGTCATTCT CGGACTCCGG ATAATCCGCC GTGACGTCAT
CCTCCATTAA GTCCGGTTTT TTACTCCGCC AGATCCGCGA GGATCTCTGC
TTTTACTGTT TTTGCGAAAA TTGAGGCGCC GTGAGGGGGA GATAACCACG
CCGTGGTCTC TTGAATCTCG CGCCCACTTA TGACTTACCT TATAAATAGG
CCGGCCCGGC ACTCTCTTCT CACACTCTCC TCTTCTTCCT CGCGCTGTCG
TTCCTCTGCC CGAGCTTCTG CCGCCGCCGC CGCCGTCGCG CCCCTGATCT
CATCGACCCG GCCGCTGCAT CACCCTGACC CGGACCAGAT AACGGCGGCG
ACCTTCGCTC TTCCTCAACT CGGTGAGTCT TCTCTTtCCT TGCTAGAACA
GATCTGTGGT AGGGTTCCTC CCGTTCTTCG TGTTCGTCAC TTTGCCCAGA
AACTCGGCAG TCCTCGTCTA GT
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wmc(1c11)
Plate reference : 1c11
WMC locus number : WMC Length of sequence : 400
Last updated : Mar 20 Juil 1999 at 14:55
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite. Bases 71 to 140 show homology to 10h1 bases 266 to 335 (complement) (70 in longest run, maxScore = 71). Duplicate with 10h1, 1c11 has priority.
PCR Comments :
Length of sequence : 400 Consensus sequence is :
..
ACTACGTGGA CTAGTGTGTG TATGTGTCTG CGCGTGCGTG TGTGTGTGTG
TGGTGTGTGA TGGGGGGACT CTACTTCGCA CTTTGCTTGG TGGCCCTCCG
TGGGGGGGGG GCTTGCGTTC ATAgCTTAGG ATTCCCCTCC GCCGACATGA
GGGGGGCAAG CAATACCGTG CGCTTTGCGA GATAGGTGCC ACATCGAAGT
TGCATTTGGT TTTGAACATC AAACCACCTC TTCATACATA TATTTGGTCC
ACATACAAGT ATGTTCGTGT TCTGACCCTT CCCGCGTCAT TTTCGCCAAA
TTTATGAACA TTAGACAAGT CGGTTGACGC AATAGACACG GTTCACTCAC
CTAACCGTGT GCCATGCCGA TGCCCTGTCA TGCACATCAG CACAGTTAGT
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wmc67(1d11)
Plate reference : 1d11
WMC locus number : WMC67 Length of sequence : 135
Last updated : Wed, Aug 20, 1997 at 11:13 am
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)8 72 to 87, small microsatellite. From microsatellite there is strong homology to 6C6 , also bases 1-70 ie. 5' side of microsatellite match the complement of part of 9e1and 9d1.
PCR Comments : 61C anneal, product;
Forward primer details :
TAggTcgATcTgTTgcAcTcTc at 26 length 22 bases, Tm 61
Reverse primer details :
TcAcTTTAgAAggTgcAAcggg at 99 length 22 bases, Tm 65
Length of sequence : 135 Consensus sequence is :
..
ACTACACACC ATATCTTTGT AGGAGTAGGT CGATCTGTTG CACTCTCTAA
CACACACATG CTGTTAAACA ACACACACAC ACACACACGC ACCTTGGTCC
CGTTGCACCT TCTAAAGTGA CTTATTACAC CTAGT
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wmc(1e11)
Plate reference : 1e11
WMC locus number : WMC Length of sequence : 358
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :
Length of sequence : 358 Consensus sequence is :
..
ACTAACTTGC CTCCCCAAAG AGGGAAGGCG GCAAAGGAAA AAGCCCTCAG
TCACTCTCGC CCGCGCCGAT CCCGTCGTCC GTCGCCGCCG CCGCCTCCGT
CCGTCCGCCA CCGAGGTAAA GAAAAAACTA GAAAAAAACA AATGGGAAAC
AGGCAGGTGC TTACCCGCGC ACGTGGGCTG GCCCCATATG CTCACTGGCT
TTAGCAAGTT GAACACTTAC TCGCTGAACG CGAGAAATAG CTATGCCCGA
AATCCATAAA TGATACGGAA GGAGCACTCA GCTAACCGAG ATATAGTCTC
CACGGAAAAG TCCTGCTCGA CCCCCGTGAG CATCAAAATG AGGTCCTTTC
CTATTAGT
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wmc(1f11)
Plate reference : 1f11
WMC locus number : WMC Length of sequence : 500
Last updated : Wed, Aug 6, 1997 at 9:09 am
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GT)8 35 to 50, (CT)8 17 to 32, (GA). microsatellites too close to end to design primers. Related to 1C10. Bases 94-133 (ie. 3' side of microsatellite are related to part of 8a1.
PCR Comments :
Length of sequence : 500 Consensus sequence is :
..
ACTAACGCGC GCGCATCTCT CTCTCTCTCT CTCGGTGTGT GTGTGTGTGT
GCGTGCGTGT GTGTGTGTGT TGTATGTGTG TGTGAGAGAG AGAGGGCGAA
ATCTCAAAAC AAAAGGTGCT CTGCTGGAAT CCCATTGTAT TATTCATAGG
GTTCCGAAAC TCGAGTTAAC CTTAGGCATA TGTGTGAGAG ACATAATTAT
ACTTTGAGAC TTATTGGGTG TGGGTGGGAA GAATTAAATG GGTGGGCGTG
TTCGACAGAG AGAGCGTGTG TGTTTCTGTG GAGATACTAG TAGGATGGGG
TAGAAAGACG AATTCTAACC TTGACGGAGG GAGAGAAATA CACGAAGTGC
CATGTGTGAT TCCGATGCCC ATAGGGAAAT GAGATATGTA TGCGTGTGAG
AGAGATTGCT CAATTCATTC CGTTTCACTA TCTAGCGATC GTGGACGTGC
ATCGCTTGAA CATAAATCAA TATCTACTTC GTATTGTGGC AAAGGTTAGT
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wmc(1g11)
Plate reference : 1g11
WMC locus number : WMC Length of sequence : 154
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : . Trimmed to Rsa1 site at 3' Originally vector sequence after this caused cross - hybridisation to 1B8, 1A7, 1F8, 1F9, 1G11. No substantial microsatellite.
PCR Comments :
Length of sequence : 154 Consensus sequence is :
..
GACGCGCGTC AAAAAcacCC aAACCAACAA AATGGCGCCG TcCTGTGCGA
CTGTGTCTGT CTGTGTGAGG CGCGTCCAtG GCAGCAGGGC AATACGTTTC
ACTGCACCAT TCTCCCAGAg CCTTACACAC CCTTTGTTGT TTGTGGACAA
GTAC
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wmc(1h11)
Plate reference : 1h11
WMC locus number : WMC Length of sequence : 6
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No sequence available
PCR Comments :
No sequence available for this clone
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wmc68(1a12)
Plate reference : 1a12
WMC locus number : WMC68 Length of sequence : 428
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GT)19 33 to 70. If polymorphic then dominant.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TAcAccTcgcgTgTgTAgccAA at 3 length 22 bases, Tm 68
Reverse primer details :
gcTcgAATTcTggcTcggcAAc at 184 length 22 bases, Tm 71
Expected size of amplified fragment (Chinese Spring=) 203 bases.
Length of sequence : 428 Consensus sequence is :
..
ACTACACCTC GCGTGTGTAG CCAAAGGAGC TTGTGTGTGT GTGTGTGTGT
GTGTGTGTGT GTGTGTGTGT TGTGTGTGTG TGTGTCTGTG TGTGTGTGTG
CAGTTCGTGT GTGCGAGCTA TCGAGTGCCA AGAGATGGCA ATGGCCATGA
GATCGGCCGG GAGCTAGAGA TGTGCCACGG TGTGTTGCCG AGCCAGAATT
CGAGCTCAAA AAAAAAGCGC CATTCGTGGC GTTCGTCGCC TGGAAAGTCA
ATGTGCTCCT TCTTTCACTT CCGTATGTGC AGTGAGAGAG AGCTGGTCTG
GGGTTTGTCC CAACAGTTGG TATCAGAGTT GGATTATATT GGGCATCAGT
CCCCAGCCGG AGGCATGCCG GCGGTGACGC CGTTCGCCGG TGGCTACGCG
ATGAAGCTGC AGGTCGTGCG CGGCTAGT
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wmc69(1b12)
Plate reference : 1b12
WMC locus number : WMC69 Length of sequence : 527
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
PIC Value : 0.21
Comments : (CT)13 352 to 377. (2-3 polymorphic loci?).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TcTgcTcTTAgcTAggAcgATg at 291 length 22 bases, Tm 61.0
Reverse primer details :
cAgATgTTccccTTcAAAATgg at 500 length 22 bases, Tm 64.0
Expected size of amplified fragment (Chinese Spring=) 231 bases.
Length of sequence : 527 Consensus sequence is :
..
ACTAACGAAG ACTAATAGCA GCTAGGTAGT AAGGATCAGG ATGGAAGATA
GTGTCGGATT TCGGGTTCCG CAGACCCTTG AAGTTCGAAC TCTCGGGTGC
GCGCGGAGAT TATGCCCTCT ACCACTCCGT CTCGCTAAGA CTAAACTACT
GAAAGAACAA CACAAGAGAC GCAAGGTTTA TACTAGTTCG GGCCACCATT
GTGGTGTAAT CCCTACTCTA GTTTGTGGTG GGTGGATTGC CTCTTGGGCT
GATGATGATG AACAATACAA GGAAGAACAG CTCGCGATGG TCTGCTCTTA
GCTAGGACGA TGAACTGCTA GGAGGAGTTC AGCCGCCCTT CTCTCTCTCT
TCTCTCTCTC TCTCTCTCTC TCTCTCTTTG TTGCTTGTAT CTTTCTAACT
AGTTTCTCGA TGCCTCTACC TAGGGGTGGC TAGTCCTATT TATAGGCAAA
GGCCCTGGGC CTCTTCCCAA ATATTGAGTG GGAAGGACGC AACGATTGGC
CATTTTGAAG GGGAACATCT GGTTAGT
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wmc(1c12)
Plate reference : 1c12
WMC locus number : WMC Length of sequence : 387
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite (individual small microsatellites).
PCR Comments :
Length of sequence : 387 Consensus sequence is :
..
ACTAACACGC CCGGCCGCTC ACACACACAC ACGCATACAC ACACACATAC
ACACACACAC TACATACACA ACACACACAC ATACACACAT ACACACTACA
CACACACACA CATACACACA GACACACAAA CACACACACA ACACACACAC
ACTACACGTA CGGTCTGATG AATGCAAAAT CTCCGAAACC CGGCGCACCC
AATGGGTTGT GACTCTCCTT CCTCCCGGAC GCAGGCAGCT AAGACGACAA
TTGACATGGG CTGCTTGCAT GTATCCTTGA ATTCTGAACA AACCGGGCTC
TCAACTCAGC CCATGACCTG ATAGAATTAG CCGGTAAACT CTCTAGCCAA
TGCGGGCCAT TCCTTCTAGC ATCATGGTGA AGTTAGT
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wmc70(1d12)
Plate reference : 1d12
WMC locus number : WMC70 Length of sequence : 295
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)22 96 to 139, (CA)24 141 to 188. (1 polymorphic locus). Close relative (duplicate?) of 1d2, 1G4 - do they detect at least 1 common locus?
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
ggggAgcAcccTcTATTgTcTA at 22 length 22 bases, Tm 63.0
Reverse primer details :
TAATgcTcccAggAgAgAgTcg at 213 length 22 bases, Tm 65.0
Expected size of amplified fragment (Chinese Spring=) 213 bases.
Length of sequence : 295 Consensus sequence is :
..
ACTAACCGGA TCCAGGGAGG CGGGGAGCAC CCTCTATTGT CTATGTTATT
AGTGTAGTAG TGCTATACTA TACGGTAGTG TGGTAGTTCT ACTATCACAC
ACACACACAC ACACACACAC ACACACACAC ACACACACAC CACACACACA
CACACACACA CACACACACA CACACACACA CACACACATC CATCAACGTA
ATGTTGAACT TCCGACTCTC TCCTGGGAGC ATTACTTAAG ATAATTTTTT
GTGTCCAATT GAATTAACTC ACCTCAGAAC GATCTCTTCG TTAGT
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wmc(1e12)
Plate reference : 1e12
WMC locus number : WMC Length of sequence : 325
Last updated : Wed, Aug 20, 1997 at 9:40 am
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite. Contains some sequence related to 1B9, 5b7.
PCR Comments :
Length of sequence : 325 Consensus sequence is :
..
ACTACcGGGt CGAGGAGCAa AGCTTAGTGC TGCTGCCGCC TGAAGTTAGA
CCTCCACTAT CCCGCCATCG CCGCGATGGA TGTGCTCGAA TCACGCCGCC
GCCGACCTCG ATGGTTGAGC TCCTCCTATC TCCCGCCGCA ACCGAGGACT
GACCTCCTAT GACGCCGCCA CCGCCCACCG ACCGAGGATT GACCGCTTCT
CCGGTAGCCG TGCCTAGGCC GCCGCCATCT CTATATGCAG AGCGGCTGTT
TTTTTACGGA TATCCGTAGA GCAGCTGTTT CACGGTATCT CACCGTAACT
CGGTTATATG GACAGGGGAG TTAGT
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wmc71(1f12)
Plate reference : 1f12
WMC locus number : WMC71 Length of sequence : 475
Last updated : Wed, Aug 6, 1997 at 9:28 am
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GA)9 354 to 371, (GT)8 194 to 209, (GT)13 312 to 337, (GT)10 391 to 410. First 120 bases have ssome homology to 1g1 family.
PCR Comments : 61C anneal, smear;
Forward primer details :
TAcgggTAAggTTggATgAAgg at 103 length 22 bases, Tm 64
Reverse primer details :
cTTAAATTAcATccgccAcAgg at 420 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 339 bases.
Length of sequence : 475 Consensus sequence is :
..
ACTAACACCT CGAGGCATGT GTACAGATGA TAAGTTTGCT GACTATTAAA
GTAAAGTGGA ATTTGTCCAT TTATGCAAGT TAATAAAGGT GATTGTTTAT
CATACGGGTA AGGTTGGATG AAGGATAGTA GGAACAAATG TCTGCCTAGA
GCATGTGTGT GTGAGATGGA GTCTTAGTGT GTGGGGGGTG TGGGTGTGTG
TGTGTGTGTG GTTTGAGAGA GAGAAAGAGG AGAGAGAGAG ATGGAGTCTT
AGTTTGTGTG TGTGTGAGAG AGAGAGAGAG GATGGAGTCT TAGTGTGTGG
GGGGTGCGTG CGTGTGTGTG TGTGTGTGTG TGTGTGTTTT GTGTGTGTGT
TCTGAGAGAG AGAGAGAGAG ATGGATTCTT AGTGTGTGGG GTGTGTGTGT
GTGTGTGTGT CGCGGGGTCC CTGTGGCGGA TGTAATTTAA GTGTGCATGG
CTTCATCATA GAGAGGTGAT GTAGT
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wmc72(1g12)
Plate reference : 1g12
WMC locus number : WMC72 Length of sequence : 311
Last updated : Wed, Aug 6, 1997 at 9:38 am
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)18 217 to 252, also CT 197-216. (2-3 polymorphic loci). Highly related to 2e10, 4c11, 6d5
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TggcTgAAAgATgTATTAcgAg at 161 length 22 bases, Tm 59.0
Reverse primer details :
AATTcAAgggTcccAcTTATgT at 266 length 22 bases, Tm 60.0
Expected size of amplified fragment (Chinese Spring=) 127 bases.
Length of sequence : 311 Consensus sequence is :
..
ACTAACGAGT TCATTGGTTT CAACATAGCC CTACCCGTAG CGATCCACTG
CTTCCATCCT GATTGTGCCA TGCGTGAATT TGTATGCAAG TTCAGTGTGC
TATGATGTTC TATATGATtG TGTCAACTAT ATAAGTTTTT TTGAGCATCA
ACAATGCATA TGGCTGAAAG ATGTATTACG AGCATCGACC AATGGGTCTC
TCTCTCTCTC CTCTCTCACA CACACACACA CACACACACA CACACACACA
CACGCACGCA CACACACATA AGTGGGACCC TTGAATTATT TATTTGCTCG
TGACAGCTAG T
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wmc73(1h12)
Plate reference : 1h12
WMC locus number : WMC73 Length of sequence : 339
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CA)9 53 to 70. (3 out of 4 loci polymorphic?)
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TTgTgcAccgcAcTTAcgTcTc at 7 length 22 bases, Tm 67.0
Reverse primer details :
AcAcccggTcTccgATccTTAg at 174 length 22 bases, Tm 67.0
Expected size of amplified fragment (Chinese Spring=) 189 bases.
Length of sequence : 339 Consensus sequence is :
..
ACTAGGTTGT GCACCGCACT TACGTCTCGA TGGCTCGAGG TTGTGCAACG
CGCACACACA CACACACACA ACACACACGG CGTTGGCGGC TCGAGGTTGT
CCGCCGCACA CACACACGGC GCCGGTGGCT TCGAGCAGCA CTCGAGGTTG
TGTGTGGCAA GCACGCACGG CGCCTAAGGA TCGGAGACCG GGTGTGTGAT
CCTGGCGCAC CCCAGAATGG ACCTATTGTA GGGTCTCTAT AGCAGGCATG
TGCTTTGGCC GACAACGGCT CTGGTGTGCC TCTGAAGATT AGTAGCTGAT
CGTTTGTTGT GGCACTAAAG ATTAGTAGTT GATGTTAGT
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wmc74(6A7)
Plate reference : 6A7
WMC locus number : WMC74 Length of sequence : 371
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Devaux, Florimond Desprez Date at Agrogene : 19.9.96 Public Domain : Yes
PIC Value : 0.49
Comments : AGR97. Trimmed to Rsa1 at 3'.(GT)34 190 to 257. (4 polymorphic loci). Related to 1E4
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
AAcggcATTgAgcTcAccTTgg at 2 length 22 bases, Tm 69
Reverse primer details :
TgcgTgAAggcAgcTcAATcgg at 285 length 22 bases, Tm 73
Expected size of amplified fragment (Chinese Spring=) 305 bases.
Length of sequence : 371 Consensus sequence is :
..
GAACGGCATT GAGCTCACCT TGGCAGCTCG GGTAGTAGCC GGGAGAGGGC
TGCGCCTCAG CCGGACGCCC CTGCAGCAGC AGCAGCAGCA CCACGGCTCC
TACCACCACC GTGAGACGTG AGATGCCAAT ATGTGGCCTA ATCTGCATCA
TCATCGTCAT CATCTTCTCT GCTCCAATAG TTGAGTGGAG TGTGTGTGTG
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG
TGTGTGTCTG TGTGTGTGTG TNNGGGGTTC TACTCCGATT GAGCTGCCTT
CACGCATCGG GCTATATGTA GTGTGGTGTA GGGTGTGGTG GGTCCCACAT
GGGTGCTAAT CTCAGGAGTA C
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wmc(6b7)
Plate reference : 6b7
WMC locus number : WMC Length of sequence : 965
Last updated : Wed, Aug 6, 1997 at 9:43 am
Owner : Devaux, Florimond Desprez Date at Agrogene : 19.9.96 Public Domain : Yes
Comments : AGR98 No overlap between forward and reverse sequences. apparently multple insert with the same sequence inserted twice ie. ABCB. 5' end trimmed to Rsa1.(GT)16 886 to 917, (GT)8 955 to 970. No room for primers. Last insert matches 1D5.
PCR Comments :
Length of sequence : 965 Consensus sequence is :
..
GTACGCCAT GTTGAGGAGT CGGAGGGTCC
AGCTGAGAAG GTGACTCGAC GTCAGTTGTG GCACCAATCA GTGTGGGGCC
GATGTCACCA ACGGAGGTCG GAGTAGACGA AGTGGTCAGG GAAGATGACG
ATGACGTTAG CGACGGGCTG GTGCTGGACG AAGAAGGAGG AGGTGGACGG
GCGACAGCTA CGGGGGTAGC GGCGACGGCG GCGGCCAAAT AAGAGCGACG
CCGACCTGAC GGCGGCGGCG GAGGCCAGGT TAGGAGCGCG GTGGCGATGC
TCGAAGTAGG CGAGGAACCC GGCAGCACGA CAAAAACCGG CGTGAACAGT
AGTCCACGCG TGGACTAACG TAGATGTAGC ATTTTTGTAT ACGTATTAAG
TCTTCGGAAA CAATAACTGC CGAACGTGCC ATGTTCGGGG CGAGACCCTT
TTTGCCGTCG GTTAAAAAGC ACGCATCTAA AAGCAAATG
(break)
GGCCAGGTTA GGAGCGCGGT GGCGATGCTC GAAGTAGGCG AGGAACCCGG
CAGCACGACA AAAACCGGCG TGAACAGTAG TCCACGCGTG GACTAACGTA
GATGTAGCAT TTTTGTATAC GTATTAAGTC TTCGGAAACA ATAACTGCCG
AACGTGCCAT GTTCGGGGCG AGACCCTTTT TGCCGTCGGT TAAAAAGCAC
GCATCTAAAA GCAAATGAGT ATGCCACATC CGTTTTGAAC ATCGTTCGGG
ATTGGTTGAA CCAGCGAACT ACTCCAACCT CGCGAGCTCG ACCTGGATCT
TCCCCACCCT TCTCCACACT CTGTCCTCAC CTCTCATCCA CTTCAAGATG
GAGGGCAGCG CATCTCGGCA AGCTCGCAGG TGAGCTCCAC AACGGCGGCG
CAAGGCTCCG GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGCGCGTGT
GCGTGTGTGC GTGTGCTTGC GCGCGCGGCG TGTGTGTGTG TGTGTAC
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wmc75(6c7)
Plate reference : 6c7
WMC locus number : WMC75 Length of sequence : 290
Last updated : Wed, Aug 20, 1997 at 1:46 pm
Owner : Devaux, Florimond Desprez Date at Agrogene : 19.9.96 Public Domain : Yes
Comments : AGR99. Fragment trimmed to insert(GT)13 117 to 142. Matches 3a6.
PCR Comments : 61C anneal, product;
Forward primer details :
gTccgccgcAcAcATcTTAcTA at 51 length 22 bases, Tm 66
Reverse primer details :
gTTTgATccTgcgAcTcccTTg at 235 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 206 bases.
Length of sequence : 290 Consensus sequence is :
..
GTCACGCTCT AACGACCCCA TGCTAGCCTA GTCAATATAT GGCACCGCTA
GTCCGCCGCA CACATCTTAC TATTTCATTT GACATTGTTC TCATTTCTGT
CCTTCCTCGT GTGTGCGTGT GTGTGTGTGT GTGTGTGTGT GTGCCCAATT
ATGTGCATGC ACATTCTGAG TTGCCTATCG CAGTTCGCTA CGCAACTCGC
CTCTTTTAGT TCTTCATTCA CACGCACCCT ATTACAAGGG AGTCGCAGGA
TCAAACAGCC CTAAACAAGT CTCCAACTAA CCTACTACGT
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wmc76(6d7)
Plate reference : 6d7
WMC locus number : WMC76 Length of sequence : 355
Last updated : Thu, Jun 11, 1998 at 11:28 am
Owner : Devaux, Florimond Desprez Date at Agrogene : 19.9.96 Public Domain : Yes
Comments : AGR100. Trimmed to insert at 3'(GT)19 140 to 177. (3 polymorphic loci?)
PCR Comments : 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
cTTcAgAgccTcTTTcTcTAcA at 97 length 22 bases, Tm 57
Reverse primer details :
cTgcTTcAcTTgcTgATcTTTg at 331 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 256 bases.
Length of sequence : 355 Consensus sequence is :
..
ATCATTGCGG CCACGGATCT CGGTGCATTC ACAATTGATC TCAAGTCCTT
GAGGTCGAGG AAGTTGTCGA GTAATTTTGC ATTCCGGCGA TATGTTCTTC
AGAGCCTCTT TCTCTACATG AGTTTCAACA CCCCACCAGG TGTGTGTGTG
TGTGTGTGTG TGTGTGTGTG TGTGTGTTAT GGTTTATATG AGTTTTTGAT
TCACCCACAT ATGTTAAGTT TCTCTTAGGG GAATCATGAA GTTTAACAAA
CGAATTCTTC TCCTTTTAAC TTGTTCTTCG AAACTGTTTT CTTCATTCAG
CGGTGGCAGG TGCTGTGGCA GAGACCGAAA CAAAGATCAG CAAGTGAAGC
AGGAC
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wmc77(6e7)
Plate reference : 6e7
WMC locus number : WMC77 Length of sequence : 338
Last updated : Wed, Aug 20, 1997 at 1:20 pm
Owner : Devaux, Florimond Desprez Date at Agrogene : 19.9.96 Public Domain : Yes
Comments : AGR101. Trimmed to insert at 3'.(GT)8 147 to 162, (GT)8 211 to 226. Software stuggled to find these primers. Bases 114-205 (across and 3' side of microsatellite ) match part of 3h1.
PCR Comments : 61C anneal, smear;
Forward primer details :
TgTgTgTTTcgTTgTggccccc at 78 length 22 bases, Tm 73
Reverse primer details :
gcTAgTTgcgAgTTTgATggTc at 257 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 201 bases.
Length of sequence : 338 Consensus sequence is :
..
GGCGTTGAGA CCCCAGTTGC AAATCGCTCA TCGACTCGCA ACTAAGGATG
TGTGCATGTG TGTGTGTGTG TAGAGGGTGT GTGTTTCGTT GTGGCCCCCA
GTTGAACCTC GCTCATCGAC TCCCAACTAA GGATGTGTGT CTGTATGTGT
GTGTGTGTGT GTTTGTAGAG GGTCACTAGT TGCATGTCGA TAACCGACTC
ACAACTAGAG GTGTGTGTGT GTGTGTCTTT NNGTCAAGAG TCCCCAGTTG
CATGTTGACC ATCAAACTCG CAACTAGCGG GTGTGTGTTT GTTGAGAATC
CCCAATTGCA AGTTAACCAT CAACTCGCAA CTAGGGTG
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wmc78(6f7)
Plate reference : 6f7
WMC locus number : WMC78 Length of sequence : 282
Last updated : Mar 22 DŽc 1998 at 17:26
Owner : Devaux, Florimond Desprez Date at Agrogene : 19.9.96 Public Domain : Yes
Comments : AGR102. Trimmed to insert. (CA)18 158 to 193. (2 loci - 1 polymorph) Bases 158 to 282 show homology to 2a3 bases 183 to 307 (125 in longest run, maxScore = 79). True duplicate with 2a3, 6f7 has priority.
PCR Comments : tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AgTAAATccTcccTTcggcTTc at 42 length 22 bases, Tm 64
Reverse primer details :
AgcTTcTTTgcTAgTccgTTgc at 261 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 241 bases.
Length of sequence : 282 Consensus sequence is :
..
GTACTAAACC TGCAAACACA AAGTCAACGT TTCCTTTCAT AAGTAAATCC
TCCCTTCGGC TTCGATTTTC TGCATGAGAC AACCGGTCGA CGACGAGGCA
CCATCAAACA CAACTTTGTA GGAGCAATTA TCAGGGCCGG CCCTGGTGAC
ACACACGCAC ACACACACAC ACACACACAC ACACACACAC ACACCCCTTT
AGCACTGGTC AGGCACAAAC AGCTGCAAAA TATTCAGTGG AACGGAACTA
CTCCTAATAG GCAACGGACT AGCAAAGAAG CT
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wmc(6g7)
Plate reference : 6g7
WMC locus number : WMC Length of sequence : 468
Last updated : 14 Nov 1996 at 15:45
Owner : Devaux, Florimond Desprez Date at Agrogene : 19.9.96 Public Domain : Yes
Comments : AGR103. Trimmed to insert at 3'. No substantial microsatellite.
PCR Comments :
Length of sequence : 468 Consensus sequence is :
..
GGGATTTTAC GTCCTGCACC GGCCACCCTG AAAAACTCGG TTGAGGCCCA
ACTGGGCCCG TTCCCCTCGC CCGTGACTGT CTGCTCTCTT CCCCGAACGC
ACCCGCAGCA CGCCGCCGTG AACAACCGCG CCACCGCCGT CGCCTCGCCT
GCCGAACTTG TCTAAAAGCT CCGCCGAGCC ACGCTGCATC TCCTCACCGA
TGAACACGAG CCCGGGAGCA CCACAGCAAG CCTTTGGGCT CGTCACCGCC
GCCTCTGCTC GCCGATCGCC GGCGTCGATT CGCCACCGTC AACGCTTCCC
CGAGCTCGCC GACGACGCCG TCGGATGCGC CGTGAGCCCC TCTCCGTTTT
CCTCCTGTTC TCCTTCTCGC GCTCGAGCCC TAGCTAGCTA TTCCGCCATG
GCTGAGATCT CGCTGCCGCC GCCGTGTAGA TCGCCATCGC TAGCCATCCC
CTAGTGCCTC GATGGTAC
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