Wheat microsatellite WMC 25/06/01

wmc(14g11) Plate reference : 14g11
WMC locus number : WMC Length of sequence : 6
Last updated : Ven 24 mars 2000 at 14:34
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : No sequence 3.11.99.
PCR Comments :
No sequence available for this clone
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wmc(14h11) Plate reference : 14h11
WMC locus number : WMC Length of sequence : 244
Last updated : Ven 24 mars 2000 at 15:21
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : True consensus shown, trimmed to insert at both ends. Bases 1 to 178 (complement) show homology to 10g12 bases 73 to 250 (178 in longest run, maxScore = 119). (CA)13 103 to 128. Duplicate of 10g12, 10g12 has priority.
PCR Comments :



Length of sequence : 244  Consensus sequence is :

..

CCACAACCTC TTGTTCCTCT TCAGACTCAA CATGGCGCTT GCATCTATGA 
GATCTCTTCT TGGACTTGGC ACGAGGAGCA AAGCAAGAAC CAGAGCCACT 
TACACACACA CACACACACA CACACACACG AGAAGCACAC AAGAGACCCA 
GCAGAGATGG GAGGATTGCA CACAAGAACA AAAGAATATA TTGCTTATGA 
ATCCGTGAAG GTAAAACAGT GCAACATGGT TCAAAAACCC CGAT

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wmc570(14a12) Plate reference : 14a12
WMC locus number : WMC570 Length of sequence : 697
Last updated : Lun 27 Mars 2000 at 16:06
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : Reverse sequence only. Bases 2 to 18 (complement) show homology to 11g5 bases 705 to 721 (17 in longest run, maxScore = 20). (GT)15 379 to 408, (GT)8 429 to 444.
PCR Comments : Polymorphic 61C anneal.
Forward primer details :
ccAAgAccAATgTTcTcATcAc at 205 length 22 bases, Tm 61
Reverse primer details :
TcTATcgTcccTcTTAAgccAg at 533 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 350 bases.



Length of sequence : 697  Consensus sequence is :

..

NGCAGGTCGA CTCTAGAGAT CCCTCCAAGG CGCGTGACTA ACTGGGTGGT 
TTCGAGGTTA TCTCACCACC AACTGCATAA AAGAGATTTT CGATGTCGGC 
GAAAACTCAG GTATTCCAGA ACTGCAACGA TAAAATTGTG ACGACAACAC 
CTCGGAGCTC AACTCCCTGG GACACTTGCC ACAACCCCTA AATGTCAGGA 
GGCACCAAGA CCAATGTTCT CATCACAAAA CCATCGGAAC GATTCCAAGA 
TACCCGCGTG ATCCTAAAAA AAATCATGAA ATTTGAGGAG AGAAAAGTCA 
AAACTTCTAC GTCAGAAGGC CTCACCAGAG CGACGAAGAG ACTGAGGAGT 
AAAAAGAATC CTACACTCTG ATATATATGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTCT GTGTCCGTGT GTGTGTTTGT GTGTGTGTGT GTGTATAATA 
TACTAAGACT CAAAACATTT TGTTCTAGAC TCAACAACGT CAGCGATTCG 
ATCGAGCAGG GGGCTCCTAA GTTGGGGATG GCCTGGCTTA AGAGGGACGA 
TAGACTACTC ATGTCAATAA GGAATTCCTT TTTTTCTGAA AGCCCATTCG 
GACAAAACTC CAAAGTTAAG CGTGCTCAGC TTGGAGTAAT TTCAGGAAAG 
GCGACCGACC CGGAAGTTGC TCCCGGGTGC GCACGAGTGA GGGCAAA

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wmc(14b12) Plate reference : 14b12
WMC locus number : WMC Length of sequence : 156
Last updated : Ven 24 mars 2000 at 15:21
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : True consensus shown, trimmed to insert at both ends. Bases 36 to 55 (complement) show homology to 21f4 bases 256 to 275 (20 in longest run, maxScore = 24). (GT)14 95 to 122. Duplicate of 21f4, 21f4 has priority.
PCR Comments :



Length of sequence : 156  Consensus sequence is :

..

CGCACTATAT GTATCATTCT GCTTTAATGC ACCTTTTTTG AATAAACAAT 
GCATAGCATT AAGACTATTA TTGCATTCTT CCTTATATAT ATATGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTTCATTAAT GACGCCTTGC AACCATACAC 
TTTGGT

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wmc571(14c12) Plate reference : 14c12
WMC locus number : WMC571 Length of sequence : 1227
Last updated : Lun 27 Mars 2000 at 16:32
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : No consensus found between forward and reverse sequences. Bases 240 to 270 show homology to 21b12 bases 285 to 315 (31 in longest run, maxScore = 188). (CT)43 828 to 913.
PCR Comments : Monomorphic at 51C anneal.
Forward primer details :
ggcTAAcTAgAgTgAggAgAAA at 779 length 22 bases, Tm 56
Reverse primer details :
gATAAgcAcgAcAAgAcATAcA at 1067 length 22 bases, Tm 57
Expected size of amplified fragment (Chinese Spring=) 310 bases.



Length of sequence : 1227  Consensus sequence is :

..

TGTGCCCGGA GAGGATTCGA ACCATATCTT CTTCACCTGT GTGTCCATNC 
AGTTCCTCTG GAGCTATTTC CGTGAAATAG TGGGCGGTAG TTGGGATGAC 
AACAATTTCC CCGGTCTCTT CACCGAACTT CAGGCATTAC CACCCTCGTC 
TCTCCACATT AGGTAGCTGA CCATCGGTGC GCTAACCTGG ACACTCTAGA 
CTGTTAGGAA TAGGCTCCTG ATTCAGCGTA TACCTCTTTG TCGCGCTACT 
AACGCTCTGT TCAAATGGTC GGGTTACTTG CAGCTTTGGC GGCCCCTTAG 
CAGTCCCCGG GAGAGAGACG CCATCACCTC CATTATCACC CAGCTTCGCT 
CGATGGCTCT TTGATGGCTC CACCGCTTCC CCTACACTGC TGGAGCCGGA 
TTAGATCTTC CCTGCGTCGN CATCGGCACC GTGTGTGTTG NGTGTGCCTG 
NGTGTCTGAN TGTTGGCTTG TCTGTTGGCC CAAAGCTAAA CCCGATTGTT 
GTGTGTGTGC TTGCCATCAG ACCTTGCCTG CGGTGGCGAT GTGTGTGTGT 
G
(break)
GGCGGGGGCA AGAGTGGGCC CCAAAGGGTG AGAACAACAG CCCAGGGCCC 
CAAAGGGGGA GGAGCCTTTC CTTTGGCGGC GGTTTTGGTT GCGGGCGGTG 
GCGGTGGAAA AACCCTAAAC CCTGTTTTCG GGGTCGGGCA AAGGGGGGCC 
ATTTAGAGAT GAGTGTGCCC CGGGCCCCCC TGCGTGTTTT GTTTTTCTGA 
ACAGAGCGTG GGTTAGCCCC GCGTGGGCTA ACTAGAGTGA GGAGAAATTT 
TTCCCCCATG TAATAAAGGC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC 
TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC 
TCTCTTGTGT TCTTGAGGTA ATACGATCTT GATGTATCGC GAGCTTTGCT 
ATTATAGTTG GATCTTATGA TGTTTCCCCC CCTCTATTCA CTTGTAATGG 
ATTGAGTTTT CCCTTTGAAG TTATCTTATC GGATTGGGTC TTTAAGGATT 
TGAGAACACT TGATGTATGT CTTGTCGTGC TTATCTGTGG TGACAATGGG 
ATATTCACGT GATCTACTTG ATGTATGTTT TGGTGATCAA CTTGCGGGTT 
CCATGAACTT ATGCATAGGG GTTGGCACAC GTTTTCGTCT TGACTCTCCG 
GTAGAAACTT TGGGGCACTC TTTGAA

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wmc(14d12) Plate reference : 14d12
WMC locus number : WMC Length of sequence : 394
Last updated : Ven 24 mars 2000 at 14:34
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to insert at 5' end, 3' end not located. No substantial microsatellite.
PCR Comments :



Length of sequence : 394  Consensus sequence is :

..

ACCGTGTTTT TTGTGTGTGT GTGAAGGCTA TCTCGATCGC CCGGGGAAGA 
ATGTGTGGGG AGAGACGGGC GGAGAAATAG GCAGGTTAGA GGTGTTTTCT 
TTGCGCCCCG CCGCCCCGGC CCCCGCACAG CAGCCGCAGA ACCACAACGA 
GGTGTTATCC AAGCCGACCC GGCCGAGACT GCGGAACTCG CGCACGGCCT 
GGAAAAACGC GCCATCACCT TCCAGAGTCA CTGACAATGG CACTAATCTA 
TCCCAAGGGG CTATGGAGGA GTTTTGTCGA CGTGAGCACA TCCGGCTTGA 
TGTTTCTTTT GTAGCTCATC CTCGATCCAA TGGTCAAGCT CAGAGAGCCA 
ATTAGGAAAT TTTGAAAGGT CTCAAACCCC GGATTATAGT TCCC

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wmc(14e12) Plate reference : 14e12
WMC locus number : WMC Length of sequence : 386
Last updated : Ven 24 mars 2000 at 15:07
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : True consensus shown, trimmed to insert at both ends. Bases 253 to 262 show homology to 5h11 bases 278 to 287 (10 in longest run, maxScore = 27). No substantial microsatellite.
PCR Comments :



Length of sequence : 386  Consensus sequence is :

..

ACGCATAGCC ACCGTCCTCC TATCGCCGCA TCGCTGGGTC TAGAAGCTCC 
ACCGTCGAGC ACCTCGTCTC CACACCGAAG GAAATGAGCC GGAGGGCTCC 
GCTTCATCGC CAATGTCGCC ATCTTCGCCG TCGGTGCCCG AAGGAAGTCA 
CCATCGCTTC GCAGCAACCT GGCCATCCCC GACCTCGTTG ACGTCGCCAT 
CGGATGCGCT GTGAGCACCC ATGCCGTATA CCCCTTCTCC CCTGGTCGCG 
CTCGTCCTCT AAGCCATAGC CCCGCAATGG CCGAAGCTCC GCCGCCGCAT 
ATGCTCGCCG CCGCCGCCGC TATAAGCCGT CCCCGGCATC CCCACGGCCA 
CCGTTGGACG CGCGTGAGTG AGGGCCTCCT CCTGGT

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wmc(14f12) Plate reference : 14f12
WMC locus number : WMC Length of sequence : 246
Last updated : Ven 24 mars 2000 at 15:07
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : No clear between forward and reverse sequences, forward sequence only shown. Bases 68 to 86 (complement) show homology to 12e8 bases 126 to 144 (19 in longest run, maxScore = 44). No substantial microsatellite.
PCR Comments :



Length of sequence : 246  Consensus sequence is :

..

TGGAGGTGCC GGATTGGCCC GGGGCGGTGG CGGCGGNNGG AGTTGCCCGG 
NTATGATGAA GAAGACCTCC CCGGGTCGAA TGCGAGGCGG NGGAGCGCCA 
TGGGGGTAGC ATGAGGTCGC GTGCGTGCTC AGAGGGGCTC GGGGTGTCCA 
TTTATAGCAC GCCGAGGTCG GTTCCGCGGN GGTGGGGATA AGGGCGGCGG 
CGAACCATCG TCTGCTGTTG CTGTTGTTGT TGTTGGTGGT GGTTGG

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wmc(14g12) Plate reference : 14g12
WMC locus number : WMC Length of sequence : 177
Last updated : Lun 27 Mars 2000 at 16:35
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : Consensus shown, trimmed to insert at both ends. Bases 8 to 142 show homology to 2g11 bases 11 to 145 (135 in longest run, maxScore = 72). (GT)14 110 to 137, (GT)5 22 to 31, (TG)5 33 to 42, no room for primers.
PCR Comments :



Length of sequence : 177  Consensus sequence is :

..

acgcagaggc acaacTAGCG AGTGTGTGTG TATGTGTGTG TGTTTGAAAC 
AGGAGTAGAT AGATTACTAT CANGATTGAG GTGCCACCAT ACTCCTTCGG 
TGTCGAGTTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGNG NGANTGTNAG 
ATTAATGNCG AACGNTAGAT GANTTAN

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wmc(14h12) Plate reference : 14h12
WMC locus number : WMC Length of sequence : 6
Last updated : Ven 24 mars 2000 at 14:34
Owner : Weining, Queensland Date at Agrogene : 3.11.99 Public Domain : Yes
Comments : No sequence 3.11.99.
PCR Comments :
No sequence available for this clone
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wmc572(15A1) Plate reference : 15A1
WMC locus number : WMC572 Length of sequence : 504
Last updated : Ven 24 Nov 2000 at 10:36
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 235 to 303 show homology to 12a9 bases 32 to 100 (69 in longest run, maxScore = 116). (CA)12 433 to 456, (CA)13 465 to 490, (GA)8 176 to 191. No matches between 15A1 primers and 12a9 sequence.
PCR Comments : Monomorphic at 61C anneal.
Forward primer details :
tgcAAATgATcATgcAcgAg at 83 length 20 bases, Tm 64
Reverse primer details :
acatgcTAAAcAgggTTTTcAcg at 353 length 23 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 293 bases.



Length of sequence : 504  Consensus sequence is :

..

GTACGTGTCC ATATGTGTGT TGTGGCGGAA GGACAATGTG GAGACGGTGT 
GCGTGTGATC GAGACATAGA GAGGTGTGAA TGTGCAAATG ATCATGCACG 
AGTGAGACAT AGACAGATGC CGATACATTG TGTATACTGA GAGAAGGGTC 
TTCTATTTTA CTTGTGTGTG TGTGTGAGAG AGAGAGAGAG AAAGGAGCAT 
CTGTAACACA ACAACAATCT CTCTTGATTC GTCTGTCCCT CGCACGATCG 
CATGTAACAC ATGCATCAAC GCACACATTT TGACACCCTC ACCCGGCCCC 
TGCCCACCTC AATGCCCGCA CATCGCTCTC TATCTCGCAC GCACAATCTC 
TTCGTGAAAA CCCTGTTTAG CATGTAGCTT TCTGTCACAT ACACACACAT 
TTCTATCCGG TTTGTTTTCT CTCAATATCT GACACACACA CACACACACA 
CACACAAACA CAAACACACA CACACACACA CACACACACA CCGCACACAC 
ACAC

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wmc(15b1) Plate reference : 15b1
WMC locus number : WMC Length of sequence : 345
Last updated : Ven 24 Nov 2000 at 10:51
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 89 to 128 show homology to 14a8 bases 254 to 293 (40 in longest run, maxScore = 76). (GA)9 172 to 189, (GA)8 208 to 223, (GA)9 254 to 271. Too difficult to make primers. Sequence contains minisatellite.
PCR Comments :
Expected size of amplified fragment (Chinese Spring=) 14 bases.



Length of sequence : 345  Consensus sequence is :

..

ATGGAGAGAA AGCGAGAGGG ACCAACAAGA ATTTTGTGTT GGATGCGTGC 
GACAGAATTG AAGATTGTCG GTGAGAGAGA GAGAGAGGGA GAGAGAGAGA 
TGGAGAGAAA GGGAGAGGGA CCAACAAGAA TTTTGTGTTG GATGCGTACG 
ACAGAATTGA AGATTGTCGG CGAGAGAGAG AGAGAGAGAT GGAGAGAAAG 
AGAGAGGGAG AGAGAGAGAG AGATGGAGAG AAAGAGAGAG GGAGAGAGAG 
AGGGAGAGAG AGAGAGAGAG ATGGAGAGAA AGGGAGAGGG ACCAACAAGA 
ATTTTGTGTT GGATGCGTGC GACAGAATTG AAGATTGTCG GCGAG

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wmc(15c1) Plate reference : 15c1
WMC locus number : WMC Length of sequence : 248
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 154 to 222 show homology to 5e12 bases 555 to 623 (69 in longest run, maxScore = 55). Complex microsatellite, too difficult to make primers. .
PCR Comments :



Length of sequence : 248  Consensus sequence is :

..

GTACGTGCGC GCGCGTGTGT GTGCTGTTGC GTGCCTATGT GCTGCCTGCG 
TGTGTGCTGC TGCTGCGTGT GTGTGTATGT GTGTGTGCTG CGTGCGTGTG 
TGTTGCTGCG TGTGTGTGTG CGCGTGCATG TGTGTTGCTG CATGTGTGTG 
CGTGTGTGTG TGCGCGTGCG TGTGTGTTGG TGCATGTGTA TGTGTTGCTG 
CGTGTGTGCT CCTGCCCTCG CACTGGTGAT GCGTGTGTGC GCTATTGC

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wmc(15d1.1) Plate reference : 15d1.1
WMC locus number : WMC Length of sequence : 226
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multiple inserts in this clone, this is the first insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 33 to 50 show homology to 15d1.3 bases 42 to 59 (complement) (18 in longest run, maxScore = 21). No substantial microsatellite.
PCR Comments :



Length of sequence : 226  Consensus sequence is :

..

ACATGGGTGC ATATGCACTT GTGCATGAGG GCCTAACCCC TCATGCTTGC 
CACTATGGTG TGATGTAGTT GTGTGCGGGG CTTGTGTGTG TGCATGTGTA 
GGTGTAGATG TTGCTACATG TGCTATTTTT ACATCTGATG TTATTCATAT 
TTAGCATATT GGATTCTAGG TGGTAGTTTT CGAGCTAGCT TTGTGCCTTG 
ATGTTACATG CAAGTTAGTC CGCGCG

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wmc573(15d1.2) Plate reference : 15d1.2
WMC locus number : WMC573 Length of sequence : 342
Last updated : Ven 24 Nov 2000 at 12:04
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)17 192 to 225, (GA)11 244 to 265.
PCR Comments : Monomorphic at 61C anneal.
Forward primer details :
gcATgcgccAcATcAAAg at 43 length 18 bases, Tm
Reverse primer details :
actcctactatgcATATAcgTAAcAcAAAcc at 303 length 31 bases, Tm
Expected size of amplified fragment (Chinese Spring=) 291 bases.



Length of sequence : 342  Consensus sequence is :

..

ATGATTCGCG AACCTCTATG TATTGCGTTT CCGGTTTGTG AAGCATGCGC 
CACATCAAAG TTGTGCATAT GGTATTAAAA CATCACACAA CACACGGGCA 
CATGCATGCA GTGGTTGTCC TATCACACTC GCTCGCATGC GACTCTCTCT 
ATGGGCCCGC AAGGTCATCG TCGATCAGTT GAACGTACAG CGAGAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAGATAGAT AGATAGATAG ATAGAGAGAG 
AGAGAGAGAG AGAGATAGAG GTTAATTTGA CCAACAAGGA TGGTTCTTTT 
TTGGTTTGTG TTACGTATAT GCATAGTAGG AGTATGTAGT AC

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wmc(15d1.3) Plate reference : 15d1.3
WMC locus number : WMC Length of sequence : 197
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multiple inserts in this clone, this is the third insert. Trimmed to insert at both ends. Bases 42 to 59 (complement) show homology to 15d1.1 bases 33 to 50 (18 in longest run, maxScore = 21). No substantial microsatellite.
PCR Comments :



Length of sequence : 197  Consensus sequence is :

..

GCACACACAC AAGGTTCATA CACCACCACA TCACCATAGT AGCAAGCATG 
AGGGGTTAGC CCTCATGCAC AAGTGCATAG GTGCACTCAT GCCCACACAC 
ACACATG
(break)
CACACACCAT ACACACACTC ATACACACTC CACCTACACA CACTAGTGCA 
TACACAAGCA CTTGCACACA CCTATATATG CTATCCTAGG

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wmc(15d1.4) Plate reference : 15d1.4
WMC locus number : WMC Length of sequence : 480
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multiple inserts in this clone, this is the fourth insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 162 to 179 show homology to 9c2 bases 163 to 180 (18 in longest run, maxScore = 46). No substantial microsatellite.
PCR Comments :



Length of sequence : 480  Consensus sequence is :

..

GTACATAGGT TCTGCTGAGG GCACAGCTCA ACAAGCCCAG AAGGAAAGAA 
AAAGCAACAC CCGCACACCA AACACAAGGC GCCTAATCAG GGTCCGGCGG 
AGGCGGCGGT GGCGGCAGCG ACAGGCGGAC GTCCATCGAA CGGATGTCGG 
CGATGAAGGC GGAGATGGCG TCACGGTCCG GGGGGGCGGC CACAATGGAA 
TGAAATGAGT AGCATCTCAC AATGAACATT TTTATATATC GTGCGCATGA 
AACAGAGCTA CGATCATGGA GTTATGATGC AATGAACAGG GGCATAAAAT 
TTACCAGGGA AAAGACTTGG AGAAAAAATT GGACCTCCAG ATCTCAGATC 
TAGGGTTCTT GGCATGGTCT ACGAGGATGG CCGGGGTTTG TCGCCGGGCT 
TGCCGGAGAA GAAGAAGGCG GACGGAGTTG GAGAAGGGAG GTCGCCGGAG 
CGCCGGATCC GGCGACCGGA GAGGAGGCGC

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wmc(15e1) Plate reference : 15e1
WMC locus number : WMC Length of sequence : 356
Last updated : Jeu 9 nov 2000 at 12:21
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 356  Consensus sequence is :

..

CATCTCTGGC TCTGATGGGC CAGCCGGATC CTCGACGACG ATAGGCTTCC 
TGCGGCGGCG CTCCGGCAGG CGGAGCACGT GGACATCGCC ATAGCGGGTG 
AGGCAGTAGA CCCTACCTTT TTCATGGTAG ACGACGTCGG CGAGCTGATC 
TCCGCTGGGG AGGCGGACGG GATCGAGGAT GGTCCACCTC CTGGCCCCCG 
CGGTGACGTA GGCGAGCCTG TCGATGTCGC AGATGGCGGC GGCGGCGAAG 
TCGTCCCTGG CGGGGTTGGG CGCGAAGACA AGCTTGGAGA CCCAGCGGCT 
CTCGTAGATC AGCGGCGGCA GAAGGATCTC CGTGGCGGTG ATGGGGTTGA 
AGAGGC

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wmc(15f1.1) Plate reference : 15f1.1
WMC locus number : WMC Length of sequence : 300
Last updated : Jeu 9 nov 2000 at 14:13
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 201 to 257 show homology to 6A1 bases 201 to 257 (57 in longest run, maxScore = 143). (CA)23 212 to 257. No room for primers.
PCR Comments :



Length of sequence : 300  Consensus sequence is :

..

GTACCAGGGG CTACATAGTA GCCCCAATAT TAGAATCCCG CGGCCAAGCG 
AAAGTGTTAC AGCTATATAG TCCGATTGCC TAGTTCGACG TGCGATCACC 
TCCTTGCCAG ACCAAGACGT TCGATAAAGA GTGATCAGGC ACATTTTTTC 
GCGAACACCC CCGCATTGTA TGCGTGGGGG CTGAAGCCAA TGACTGCTAT 
CTTTCGGATT ACACACACAC ACACACACAC ACACACACAC ACACACACAC 
ACACACAGAG ACACACGCAG CACAACACAC ACACACACAC TCCCCCGAGC

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wmc574(15f1.2) Plate reference : 15f1.2
WMC locus number : WMC574 Length of sequence : 234
Last updated : Lun 27 Nov 2000 at 15:00
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (CT)24 148 to 195.
PCR Comments : Polymorphic at 61C anneal.
Forward primer details :
TccccTAcTggAAccAcgAc at 53 length 20 bases, Tm 64
Reverse primer details :
ATccATcgAccgAcAAgAgc at 199 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 166 bases.



Length of sequence : 234  Consensus sequence is :

..

GGCCTGCTGC TCCTAATCCA CCGCCGGGCC TTCGTGTGCC TCCGCGATGA 
GCTCCCCTAC TGGAACCACG ACCACGGCGA CGGCGGCACC AACAAGTTCC 
TCCTCTTCGC CTCCGACGTC AAAGATAATC TGGTAACAAC CCATCCCCTC 
TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTTCTGC 
TCTTGTCGGT CGATGGATTC AGTTTCCTCT GTAC

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wmc(15g1) Plate reference : 15g1
WMC locus number : WMC Length of sequence : 612
Last updated : Jeu 9 nov 2000 at 14:14
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 2 to 456 show homology to 14e4 bases 2 to 456 (455 in longest run, maxScore = 291). (CA)9 11 to 28, (CA)9 240 to 257, (CA)10 463 to 482, (CT)8 115 to 130, (CT)10 322 to 341. Too difficult to make primers. Sequence contains minisatellites.
PCR Comments :



Length of sequence : 612  Consensus sequence is :

..

GGACCCTCGA CACACACACA CACACACACG CATGCACGCG CGCGCGCGCA 
CACACACACA CATCCCCTAG TTGCGAGTTG GTGGTCAGCT TGCAACTGGG 
GGCCACGAAT AAAACTCTCT CTCTCTCTCT TTATCTAGTT GCAAGTCGGT 
TATCGACATG CAATTGGGGA CCCTCAACAC ACACACACAT CCCCTAGTTG 
CGAGTTGATG GTGGACTTGC AACTAGGGCC ACGAATAAAC ACACACACAC 
ACACACACCA CACCTAGTTG CGAGTTGGTG GTCAACTTGC AACTGGGGGG 
CATGAATAAA ACACACACAC ACTCTCTCTC TCTCTCTCTC TGTGTGTGTG 
TGTCTGGTTG TAATTCGGTT ATCGACATGC AGTTGGGGTC CCTTGACACA 
CACACACACA TCCCCCTAGT TGCGGGTTGA TGGTCAACTT GCAACTAGGG 
GCCATGAATA AACACACACA CACACACACA CACTAGTTGC GAGTCTGTTA 
TCAACATGCA ATTGGGGACC CTCGACACAC ACACACACAG TCACACACAC 
CAGGTTAATG ATGAAATCCC TTAAGATAAA ACAAACAACA GAGGAACAAA 
CCAGCTGCGT AC

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wmc(15h1) Plate reference : 15h1
WMC locus number : WMC Length of sequence : 507
Last updated : Lun 27 Nov 2000 at 15:40
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 507 show homology to 9h5 bases 1 to 507 (507 in longest run, maxScore = 414). (GA)17 415 to 448. 15H1 is a duplicate of 9h5, 9H5 has priority.
PCR Comments :
Expected size of amplified fragment (Chinese Spring=) 142 bases.



Length of sequence : 507  Consensus sequence is :

..

GCGCTCCTCG GAGTTTCGGC AGTGGCGAGC TCCGGTTGAC AGTGAACTCT 
GTGCTCCCAG GTATTCTGGG CATCGGGGTA GTGTCTAGGA GGGTTGCCGG 
GGGGTGGGGT TCATGTGGTG GGGACAGTTG AGCTTAGGGT GGTCGGGGTT 
CGCCGGAATT CGATCGAAGA TCTCTTGGCC AAAGCATATG AGACGGTGAC 
GTTACGGGCA CAGCCTAACG GTGTCAGGCG AAATAGGTAG GTAGCTTTTT 
CATAGGGAGC TAGGGGGTGT TTATAGGGCA TGGCGGGTCG GGCGGGCATG 
CATGGTGGCC ATGAGTGGAG GTCGTGGGCG CTCGGGTTTG TGCGCGGTGT 
GGGCGCGCCA GGCGGCAGTT ATGTGAGGTG GGCGTCAACC CTGATGGGTG 
CGTCCCGCCT GGCAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGATG 
TTGGGGAATG CAGTAATTTC AAAAAAAATC CTACGCACAC GCAAGATCAT 
GATGATG

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wmc(15a2) Plate reference : 15a2
WMC locus number : WMC Length of sequence : 295
Last updated : Jeu 9 nov 2000 at 12:21
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 295  Consensus sequence is :

..

GGGAGTATAG CTGGGAGAGG GAATTGGCGG CGCCGCAGAA GCTGGAGTAG 
AGCGTGCGGT CGGCCTCGTC CAACCGCGGC GCGGCGTCCG TCTTTCTCTT 
TCGGGCCATC GCCGCCGCCG CCGCCGGAGA TCCCCTTAGC CACCGAGGAG 
AAGGATGCGG AAGAGGGTTT GATGCGATTT TTTCCCCTTT TCTTTTTTCT 
TTGTTCTACG GGTGGGCGGT GTGGCGTGCG GTGACCATGC CCTTTCAAAT 
CAAATCTCGT GTGTATCGGT TTTGGTTTTG GTTTTGGTTT GGTAC

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wmc(15b2) Plate reference : 15b2
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 9 nov 2000 at 12:21
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No sequence 27.10.00.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(15c2) Plate reference : 15c2
WMC locus number : WMC Length of sequence : 594
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert. Bases 355 to 432 show homology to 24h2 bases 1 to 78 (78 in longest run, maxScore = 126). No substantial microsatellite.
PCR Comments :



Length of sequence : 594  Consensus sequence is :

..

GTACGGAACC ACCTCAAAGT TATTCTTTCC GATCAATCCA TTGGGCTATT 
CCTATAAGTG TCACAAACAG CCCTAGAGTT CGTATTAAAA TAACACCTTA 
AGACACAAAT CAACCAAAAC CCTAATGTCA CCTAGATACT CCAATGTCAC 
CACAAGTATC CGCGGGTTTG ATTATATGAT ATGCATCACA CAATCTCAGA 
TTCATCTATT CAAACCAACA CAAAGAACTT CAGAGAGTGC CCCAAAGTTT 
CTACCGGAGA GTCAAGACGA AAACATGTGC CAACCCCTAT GCATAAGTTC 
ACAAGGTCAC TAAACCCGCA AGTTCATCAC CAAAACATAC ATCAAGTAGA 
TCATGTGAAT ATCCCATTGT CACCACAGAT AAGCACATGC AAGACATACA 
TCAAGTGTTC TCAAATCCTT AAAGACTCAA TCTGATAAGA TAACTTCAAA 
GGGAAAAATC AATCCATTAC AAGAAGGTAG AGGGGGAGAA ACATCATAAG 
ATCCAACTAT AACAACAAAG CTCGCGGTAC AGGTGAAGGA TACACCTTGA 
AATACGAAAA ACTTACCGGG CTGGGGGGAT GGGCCAGGTT GTAC

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wmc575(15d2) Plate reference : 15d2
WMC locus number : WMC575 Length of sequence : 485
Last updated : Lun 27 Nov 2000 at 16:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)5 179 to 189 (GT)9 195 to 212.
PCR Comments : Monomorphic at 61C anneal.
Forward primer details :
tcttgcTcATAggTccTgATTcTTg at 149 length 25 bases, Tm 64
Reverse primer details :
tcagttcAgTgcTTcAgAcATTAgTg at 402 length 26 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 279 bases.



Length of sequence : 485  Consensus sequence is :

..

CACTACTTCA ACACAGAACT ATTTCCAGGA TAATTGTTTC TTCAATAACC 
CAGATCTTCT TAACCAAATA TCAGGATCCT CTCCTTCAAA TCTAGGAAAA 
TCCATCTTAG GGCCCTTGGT CAAAGACTTC ATGAATTGGG CTTGCATATC 
TTGCTCATAG GTCCTGATTC TTGCCATTGT GTGTGTGTGC GTGCGTGTGT 
GTGTGTGTGT GTGCACGCGC GCGCGCGCAA ATGCATCTCT TCAATTTGTT 
TACATTTTTC CTTTTCTGCC TCTAATTGTT TCCTTAGTTG CTTCGTTCTA 
TTCGGCTGGT CATGCACTTG CTCTTGTTGT AGTGTAGGAG ATTTCACCAA 
TTGACTAGAA CTGGAAGGAA TTTCATTATG TGGGTTGCTG TTCATTGGTT 
TCACTAATGT CTGAAGCACT GAACTGATTG TTAAGAGTTG CTTGCTGAGA 
TCTAGCACAA CCTTCTGCAC ACTACCCACT TGTAC

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wmc(15e2) Plate reference : 15e2
WMC locus number : WMC Length of sequence : 336
Last updated : Jeu 9 nov 2000 at 14:20
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)27 1 to 54. No room for primers.
PCR Comments :



Length of sequence : 336  Consensus sequence is :

..

CACACACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CACAAAGGGC TCACAAGTTA AATTTTTGGA AAGGTCTTCT AGCTCTATAT 
ACGTATGTGG TTCCAACCAT TGTCCAAGAC TTGTGGCTAC CATTTGATTC 
CATTGAGCGG GCTCTTGACG GTGTCGGGAC CTTGTTGATG TTGGCGGTTG 
TGCTTTGATC TGCTCCTCGT ATACATGGTA ATTTCTGGTT GTGATGTCCA 
CGTCAGGGCT GTGAGCGTCG TCTGATGTCG AGGGACACTC TGGCTTGCAG 
CAGGGACTTC CTCGGTGAGA GTGGGGGTGA CAGTAC

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wmc(15f2) Plate reference : 15f2
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 9 nov 2000 at 12:21
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No sequence 27.10.00.
PCR Comments :
No sequence available for this clone
Go back to index

wmc576(15g2) Plate reference : 15g2
WMC locus number : WMC576 Length of sequence : 497
Last updated : Lun 27 Nov 2000 at 16:24
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 471 to 497 (complement) show homology to 13h6 bases 10 to 36 (27 in longest run, maxScore = 113). (GA)13 169 to 194, (GA)27 266 to 319. Forward primer has 8 mismatches with 13h6, reverse primer has one mismatch.
PCR Comments : Monomorphic smear at 61C anneal.
Forward primer details :
gcATAcAATgAgTgcAcccAA at 43 length 21 bases, Tm 64
Reverse primer details :
tTgATgcATAAAAcgcgAccT at 341 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 319 bases.



Length of sequence : 497  Consensus sequence is :

..

GTGCATGAGA GAGTTACGGG CAACGAGCCA AGGAATCTTT GTGCATACAA 
TGAGTGCACC CAAGATATTT ATCTTAGACT AGAGAATCAT ACATAGTGTG 
CGAGAGAGAC CTATAGATGG AGTATATATG CATGCGAACT AGAAAGACCC 
CCCCCACACA CACACAAAGA GAGAGAGAGA GAGAGAGAGA GAGATGGAGA 
GAAAGGGAGA GGGACCATCA AGGTTTTTGT GTCGGATGCA TGTGACAGAA 
TTGAAGATTG TCGGCGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGAGAGAGAA CAGGAGTCCG GGAGAGGGGG AGGTCGCGTT 
TTATGCATCA AAGACTGATA TAAACCATGT TTCGATATAA ACGTGCATTC 
AAATATTTAA ATTGGAGAAC ATGTTGTCTG CAATCCACAC ATGAACGGAT 
ATATGCGTAT ATCAAACAAG TTCATTAATT TGACTTTCAA CATGTTC

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wmc577(15h2) Plate reference : 15h2
WMC locus number : WMC577 Length of sequence : 232
Last updated : Lun 27 Nov 2000 at 16:37
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 232 show homology to 15e5 bases 1 to 232 (complement) (232 in longest run, maxScore = 184). (GCC)6 84 to 101. 15h2 is a duplicate of 15e5. 15h2 has priority.
PCR Comments : Monomorphic at 61C anneal.
Forward primer details :
cTgTccgAcTccccAgATg at 37 length 19 bases, Tm 64
Reverse primer details :
cccTgTcAgAggcTggTTg at 142 length 19 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 124 bases.



Length of sequence : 232  Consensus sequence is :

..

GTACTTTCCA ATCCAGCCGG GCGATCTCGG GTCCGGCTGT CCGACTCCCC 
AGATGAGACA GGGGCATTCT CCATACTCCC CTCGCCGCCG CCGCCGCCGC 
CATGCATGCT CTCCGCCACG GAATACAACA CTCTGAGGCG TCAACCAGCC 
TCTGACAGGG CACACGCTTT TTTTCCCTAG CCAACGGGCA CGCACACAGC 
TACGGAACAC AAAAAATTCT GGACCACGGT CC

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wmc(15a3) Plate reference : 15a3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 9 nov 2000 at 12:21
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No sequence 27.10.00.
PCR Comments :
No sequence available for this clone
Go back to index

wmc578(15b3.1) Plate reference : 15b3.1
WMC locus number : WMC578 Length of sequence : 509
Last updated : Mar 28 Nov 2000 at 10:19
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at 5' end and to Rsa1 at 3' end (NB 5' linker corrupted). Bases 276 to 310 (complement) show homology to 22a5 bases 143 to 177 (35 in longest run, maxScore = 113). (GT)9 129 to 146. Reverse primer has two missmatches with 22a5 sequence. Forward primer is unique.
PCR Comments : No amplification at 61C anneal.
Forward primer details :
gAAgAgcAATgccTTAgccg at 84 length 20 bases, Tm 64
Reverse primer details :
AcATAgccAgATgccgATAcg at 389 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 326 bases.



Length of sequence : 509  Consensus sequence is :

..

AGGAGGAGGT GCGCGCTTGC TTTTCAGTAA GAAAATGAGA GAAAAAGGGT 
AGGACCGGCG ATCAAATCCA GGTCTTCAAT GTGGAAGAGC AATGCCTTAG 
CCGTTGCGGG GTGTGTGTGT GCGTGCGCGT GTGTGTGTGT GTGTGTCAGA 
TATTGAGCGA AAACAAACCT AAGGAGAGAA ATGGGTATTC ACGAACAGAT 
TGTGCGGGCC TTTAGGTGGA CAGGGGCCGA GTGAGGGTGT CAAAATGTGC 
GCGTTGGTGC ATGTGTTGCA TGCGCTCATG CGAGGGACGG ACGAATCGAG 
AGAGATGGTT ATTGTGTTAC AGATGCTCCT CTCTCTCTCA CACACACACA 
CAAGTAAACT AGAAGACCGT TCTCTCATGT ATACACAACG TATCGGCATC 
TGGCTATGTA TGACTCAAGC ATGATCATTT GCACATTCAC ACCTCTCTAT 
GTCTGTATCA CACGCACACC ATCTCCACAT TGCCCTTCCG CCACAACACA 
CATATGTAC

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wmc(15b3.2) Plate reference : 15b3.2
WMC locus number : WMC Length of sequence : 46
Last updated : Jeu 9 nov 2000 at 12:21
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 46  Consensus sequence is :

..

TCGCGGACAT ATAAAGAATC ATATCAAGCA AGCGTGGTAT ATATGC

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wmc579(15c3) Plate reference : 15c3
WMC locus number : WMC579 Length of sequence : 296
Last updated : Mar 28 Nov 2000 at 10:33
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert. (CA)10 63 to 82, (CA)23 85 to 130.
PCR Comments : No amplification at 61C anneal. Faint monomorphic several bands at 51 C anneal.
Forward primer details :
gtaccgaTTAccAggAAgAcATAcATc at 1 length 27 bases, Tm 64
Reverse primer details :
ggtccTTggAAcATcTcAgAAATc at 236 length 24 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 259 bases.



Length of sequence : 296  Consensus sequence is :

..

GTACCGATTA CCAGGAAGAC ATACATCTGT GAATTTCTTT GATGTGAGCA 
AACTGATTCC AACACACACA CACACACACA CATACACACA CACACACACA 
CACACACACA CACACACACA CACACACACA TGCACACTCG CACACACTAC 
CATATGTGAT GTTATGCAGG CCATAGACAT ATATTTCACT ATTTATAGAC 
AAAAACTTTG AATTCTTTGG GTGGTAAAAC TCGATGATTT CTGAGATGTT 
CCAAGGACCA GTTCTTTTCC CGCCTCTTGG AAAGAGGCTC CGGTAC

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wmc(15d3) Plate reference : 15d3
WMC locus number : WMC Length of sequence : 476
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 252 to 359 show homology to 5A7 bases 3 to 110 (108 in longest run, maxScore = 186). No substantial microsatellite.
PCR Comments :



Length of sequence : 476  Consensus sequence is :

..

GTACTGATGC GATAGATAGG CTAGGGGTCC TCCTTAATGT CCACAGGAAC 
ATCTGTGAAA ACTTCACACC AAGCAAACAG CAAGAGCCAT TCGTGAAATT 
CTCGGCACAA GACAGTTAAC TTAAACCTCA TTTTCATGTC ATAGCATCGC 
CAGCGACGAC TCAGATGTCA CACGACAAGC AAAAAATGGA TAGCGGTAAT 
AGGATCCGAA TAATGGTGCA CCCAGAGAGG GGCAGTCTGC CTGTTGAGTT 
GTAGTAGACA GTATGATGAC ACATGGTGCT GCTCTATGAG CAGGAGCTGC 
CCGCTTGCTC GCTGGCAGGC GGAGGCGGAG GCGGAGGCGG AGGCGATCAG 
ATCTCATAGT TCCACGTGCT CTCCGCTACG AGCTTCGGTG CTTTGCTTTG 
ATCGGACAGA TCGAACGTCG TCGCAACCTG CACAGACAGA GCACAGCAGA 
ATCAGGTCAA AAAGCGTTAG TCCACG

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wmc(15e3) Plate reference : 15e3
WMC locus number : WMC Length of sequence : 30
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Apparently no insert. Bases 1 to 30 show homology to 1F2 bases 1 to 30 (30 in longest run, maxScore = 22). No substantial microsatellite.
PCR Comments :



Length of sequence : 30  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC

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wmc(15f3.1) Plate reference : 15f3.1
WMC locus number : WMC Length of sequence : 192
Last updated : Jeu 9 nov 2000 at 12:21
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 192  Consensus sequence is :

..

CGAAACAATC ATAACTGGTA TAAAACAGAG TGACCAAGTT ACAGAGTAAT 
CTCTAATTCA TCAGAAGTTG TTAAGTAGCA ACTTAATTTG ATTCTACAAG 
ACACGCACTA GAAATATCGA TTTCTCATCC AATTGACAAC TAAATTCAAT 
GCACAACTTT TGGTGGTAAC TGATTGGTGC GCGCGCGCGC GC

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wmc(15f3.2) Plate reference : 15f3.2
WMC locus number : WMC Length of sequence : 305
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 216 to 232 show homology to 6a4 bases 234 to 250 (17 in longest run, maxScore = 67). No substantial microsatellite.
PCR Comments :



Length of sequence : 305  Consensus sequence is :

..

GTACCTGTGG CATTTCCTCG AACACCACCT TGGACCCCCT CAACACCCCC 
CCAAATCCTT CGCTCACCCC AATCCGCCGC TCACACACAT ACACACCACC 
CCTCCCTTGC TCCAAACATA GCTAGGTCGC CGCCGCCATG CCGCCTCCAT 
CGTCAACCTC TGCCGGTCTG CCCATGTAGT TTACCCACCG ATATACATAC 
CCTCGCTGCC CTGAATTTCT TAGATGACGC CTACGAAACG TCCCCTTTCC 
CAGAGATCCC CATCCCCAGC CCTCGCCCAC TCCAGAGCGT CTCAGCCTAA 
GCCGG

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wmc(15g3) Plate reference : 15g3
WMC locus number : WMC Length of sequence : 252
Last updated : Jeu 9 nov 2000 at 12:22
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end (NB 5' linker corrupted). No substantial microsatellite.
PCR Comments :



Length of sequence : 252  Consensus sequence is :

..

AGGGTTTTCC GGACGTCCCC GCCTCCTTTT CTGACCGTTG GTGCGTGGCC 
TCACCTTCCA AATTTGTTCT TCAAGACGAA AGAGAAAAAA GAAGCCCAGC 
CCAGTAGCCC ACGCAGGGCA GTCGTCCGTC TTCCAATCCG CCGCCGCCGT 
CTCCAAGAAC ACGGGCAGGG AACTGAAGAA CCAATCCGCC GCCGCCGTCG 
CCCCCTCATC CCCGCCTCGT CTCCAGGAGC ACTCACGCAC GGGTTCGTGT 
AC

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wmc580(15h3.1) Plate reference : 15h3.1
WMC locus number : WMC580 Length of sequence : 552
Last updated : Mar 28 Nov 2000 at 10:52
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to Rsa1 at both ends of insert. (CA)23 389 to 434 plus numerous small CA repeats.
PCR Comments : Polymorphic at 61C anneal.
Forward primer details :
AAggcgcAcAAcAcAATgac at 178 length 20 bases, Tm 64
Reverse primer details :
ggTcTTTTgTgcAgTgAActgaag at 483 length 24 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 329 bases.



Length of sequence : 552  Consensus sequence is :

..

GTACACGTAA GGTTGATGAT TTTTGGGCTT GGAGTAAGGA CAACCGAGGC 
ATCTTCTCGC TGCAGTCTGC CTACAGGGTG ATGGTGCATA TATACTAAAT 
TGAGTAGAGA GAGATGGCTA GAGAATCAGC GAGGGGGCTC AGATCAGGAT 
AGATAGTAGA TAGCATACGA TTTAATGAAG GCGCACAACA CAATGACATT 
CCAGAGAAAA GCTCAAAACA ATACAAGGAG ACATTAGGGA GATGTTCAGA 
TAAACTTAGA ACCGTAATTT CAGAAATACA ACTCAGTTTT GAAACACACA 
CACGCACACC CACACACGCA CACGCACACA CGCACACGCA CACACGCACA 
CGCACACGCA CACGCACACA CGCACACGCG CGCGCGCGCA CACACACACA 
CACACACACA CACACACACA CACACACACA CACAATCAAC ATATAAGTTT 
CAAACTGTCA AATGAACACA CTATTGAAAA CACTTCAGTT CACTGCACAA 
AAGACCAAAA GATCAAAAGA TCAATTGACA ACAAGAATCA GTTACGGAGT 
AC

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wmc581(15h3.2) Plate reference : 15h3.2
WMC locus number : WMC581 Length of sequence : 161
Last updated : Mar 28 Nov 2000 at 10:58
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 104 to 161 (complement) show homology to 6g11 bases 194 to 251 (58 in longest run, maxScore = 55). (GT)23 36 to 81, (GT)8 83 to 98. Reverse primer matches 6g11 sequence. Foward primer is unique.
PCR Comments : Polymorphic at 61C anneal.
Forward primer details :
cATgTTgccATcAAAcTcgc at 10 length 20 bases, Tm 64
Reverse primer details :
gctaTTgAcATgcAAcTATggAcct at 108 length 25 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 123 bases.



Length of sequence : 161  Consensus sequence is :

..

GCCTAGTTGC ATGTTGCCAT CAAACTCGCA ACTACGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TTGTGTGTGT GTGTGTGTTT 
TCTTTCTAGG TCCATAGTTG CATGTCAATA GCAGTCTTGC GACTAGGAGT 
GCGTGCATGT G

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wmc582(15h3.3) Plate reference : 15h3.3
WMC locus number : WMC582 Length of sequence : 256
Last updated : Mar 28 Nov 2000 at 11:10
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the third insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 10 to 27 show homology to 24b5 bases 135 to 152 (18 in longest run, maxScore = 38). (GT)30 118 to 177. Foward primer has 7 missmatches with 24b5 sequence, reverse primer has 3 missmatches with 24b5 sequence.
PCR Comments : Polymorphic at 61C anneal.
Forward primer details :
gAgATTcccTcTcTcgccAA at 50 length 20 bases, Tm 64
Reverse primer details :
ctagcAAAgTgcgATgTAgTccc at 182 length 23 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 155 bases.



Length of sequence : 256  Consensus sequence is :

..

GTACGATCGA GGTGGATTCT TCTTGGTATG TTTTACGATA CATCCTGGTG 
AGATTCCCTC TCTCGCCAAC TCAAAGAGTG TGTGTGGGTG GGTGGGTGTG 
TGTGCGCGCA CGTGCATGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTTTG AGGGACTACA TCGCACTTTG 
CTAGGTGAAC CTCCATGGGG GCCGGGGATG TCTGGTTAAT TTGCGAGGCA 
CATGTG

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wmc583(15a4) Plate reference : 15a4
WMC locus number : WMC583 Length of sequence : 387
Last updated : Mar 28 Nov 2000 at 11:24
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 301 to 363 show homology to 17h7 bases 245 to 307 (63 in longest run, maxScore = 157). (CA)8 102 to 117, (CA)9 128 to 145, (CT)12 78 to 101. Reverse primer has 3 missmatches with 17h7 sequence. Foward primer is unique.
PCR Comments : Monomorphic at 61C anneal.
Forward primer details :
caTAcAATgggATTccAgcAgA at 37 length 22 bases, Tm 64
Reverse primer details :
aATAAggcATTcccTcgTTcAtc at 362 length 23 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 348 bases.



Length of sequence : 387  Consensus sequence is :

..

CCTAAGGTTA ACTCAAGTTC CGGAACCATG TGAATACATA CAATGGGATT 
CCAGCAGAGC ACCTTTTGTT TTGAGATCTC TCTCTCTCTC TCTCTCTCTC 
TCACACACAC ACACACACGC GCGCGCGCAC ACACACACAC ACACATTGTT 
TCTCGCTTCA TTTTTCCGGC ACATGCATGC TCACCGTTTT GTTTATCTTC 
GTGATGCTTT AAGTATGCCT CGCACAAATG CTATCTACCT ATCCCTTAAT 
ATAGCTAGAT ATGTGTCACA CAAACTCCTT CTCCCTACTA GCAAGATGCC 
CATGCGTTGC ACGGAACATC AAGATGCATT TGTATGAGTA GTTTATCTTG 
TGGGAGAAAA GGATGAACGA GGGAATGCCT TATTTGC

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wmc(15b4.1) Plate reference : 15b4.1
WMC locus number : WMC Length of sequence : 309
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 67 (complement) show homology to 12c9 bases 284 to 350 (67 in longest run, maxScore = 227). No substantial microsatellite.
PCR Comments :



Length of sequence : 309  Consensus sequence is :

..

GGGAATCGAG AGGGTGCGGG CGGTCGGCGG CGGCGGCCGG TGGACCGGAT 
CTGGCGAGGT GGGATAGTGA TCGAGATGGA GGGGGATCGA GATCGAGTGT 
TCATGAAATT AAAAAATATT CATGATAGTT CAAAAAGTGC CCATGAAATA 
CAATCGAGAA ATGAAGTCTA TGATTTAAAT ACAATCGATC TTAGCTAGCT 
ATCTGTTCAC AATCTTCTTG CCCTTCTTTA TCGCAAATGG AGTTCTTCTA 
TGGAACGGAT GTCCTTTAGG TAGGGTGGTC CTGCTTCTTC TTGTGGTGTA 
TGCTGGTAC

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wmc(15b4.2) Plate reference : 15b4.2
WMC locus number : WMC Length of sequence : 354
Last updated : Jeu 9 nov 2000 at 12:22
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 354  Consensus sequence is :

..

CCTGTCATGT AAGGCTGCAA TGGCAGCAGC AACCTGAGAG ACAAAAATAG 
GGCATGTCCC AGTATCTCTC AACTCACATC CTTTTTCACT AGCGCCTCCA 
GCAACACCAT CTCCAACACC GACAGCCAAA CAAGACCCCA ACCTAAGAAT 
TGCCTCCTTC ACCGCCGTAA TCGCGAAGAA CCAGCCATTG CCCTTCCCAT 
ACAAAACCTC GAGCTGGCTC GCCAACCAGG AGACACCATC AACCAGCCTG 
GGGCACTCGA ACCGCACAGG CCTCGGATCA AGACGAAACT CCTCGCCCTC 
CCCCTTGGGC AGGCGTTCCA GCGAGCACCC TGCCTCCGCC GCCGCCGCCT 
TCAT

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wmc(15c4) Plate reference : 15c4
WMC locus number : WMC Length of sequence : 338
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 103 to 147 (complement) show homology to 10f10 bases 192 to 236 (45 in longest run, maxScore = 209). No substantial microsatellite.
PCR Comments :



Length of sequence : 338  Consensus sequence is :

..

GCAGTCTAGG TCGTTGGATG ACAAGGAATA CATGTGGCTT GATGACGTTT 
TATATTTAAT TTGATACGGC TCTAACAGAA CATTCAATCA ATCAAACCAT 
ACATTTCGTT CAGTCTGACT CCGACTTTAA ATTATACGAC GATCGATATA 
AAATAAATGG AAATGATAAA CGTTCGGATT TTAAGCATGG CAATCCGAAC 
GTTTTTCATG GAAAATTTAG ATTACGCGGC GGCGGCGGGG GCGTCTCGCG 
GTGGGAAGCG GCTCCTCTGC CGTGCTATGT GTGTCGTCGG GCCACTGACC 
GATGGCGACG GCGTCGTCTT GCGCCGTTGC TTGCGTAC

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wmc(15d4) Plate reference : 15d4
WMC locus number : WMC Length of sequence : 153
Last updated : Jeu 9 nov 2000 at 12:22
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 153  Consensus sequence is :

..

GTACAGGGAG CTGTGAACAA AACCCTGTTT TCTACTAGTG TGGGGAGAAA 
GCGGCAGCGG TGGAAGGGAG AGAGGAGGCG GCGGCGTGAG ACGCGGCGGC 
GGCCTAGGGC GGAAGCGATG AGATCGGTAG AAAACTGATA CCATGTAGAA 
GAC

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wmc584(15e4) Plate reference : 15e4
WMC locus number : WMC584 Length of sequence : 244
Last updated : Mar 28 Nov 2000 at 11:45
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)16 84 to 115.
PCR Comments : No amplification at 61C anneal. Monomorphic several bands at 51 C anneal.
Forward primer details :
gtacggTcAgcTAgcTgTTTgTg at 1 length 23 bases, Tm 63
Reverse primer details :
ggAcTTAAgTgccATgcgAAg at 172 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 192 bases.



Length of sequence : 244  Consensus sequence is :

..

GTACGGTCAG CTAGCTGTTT GTGGATCGCC TTGGGTGTCG CTAGGGAAGG 
GACAAAAATG GCTAAGCCAG AGAGAGAAGG TGGGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTAGGAT GAGGAGAAAA GGAGCAGGGC GGTAGGGGGT 
ATTTATACAA TCGATTTGAG CCTTCGCATG GCACTTAAGT CCTGTTAATT 
ATCTGAACAA AATGCGTTGC CTAAATATGA GGCTCATTGC TTTC

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wmc585(15f4) Plate reference : 15f4
WMC locus number : WMC585 Length of sequence : 526
Last updated : Mar 28 Nov 2000 at 13:19
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 141 to 167 show homology to 11e11 bases 175 to 201 (27 in longest run, maxScore = 108). (GT)11 414 to 435. Foward primer has 3 mismatches with 11e11 sequence, reverse primer has 2 missmatches with 11e11.
PCR Comments : Monomorphic, very stuttery, at 61C anneal.
Forward primer details :
TgcAcgggcAAAccAg at 290 length 16 bases, Tm 64
Reverse primer details :
cAgTAgccAcgTcATTTccTca at 457 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 189 bases.



Length of sequence : 526  Consensus sequence is :

..

GACCTTGATG TCGTAGCCAG CGTCCACGAC ATACCGGCAT ACTCTAGCCC 
GCTGATTGAG TGCCTGCGGC CAGTAAAGGT CGTCAGCTTC CTCGGTGACG 
TTGAGCGAAG AGACCGCGAT ACACCTCTTT TGGGCCAGTC GCTCCTCGAG 
CTCCACGGGA AGCGTAGACA GGCTTAGGTT GTGACGCTCT GGAGCGGGGA 
TGCGGATGGG GATCTGTGGG AAAGGGGTGT TTGCCAGGCA TCATCTAAGA 
AACTCAGGGC GGCCTGGGTA TGGAGATCGG TGGGAAAGTT GCACGGGCAA 
ACCAGCAGAT GTTGACAATG GAGGCCTTGC GGCGGCGGCG GCGACCTTAC 
TAGGGTTTCG AGAAAGGGAG GGGGTGTGTG TGCGCGCGCG CGTGGGCGCC 
CGAGGAGGTT TTGGTGTGTG TGTGTGTGTG TGTGTGCGGA GGGGGGAGGG 
GGTGTTTGAG GAAATGACGT GGCTACTGAA AATTTTACTA CGCGGGAGTT 
AAATGCCGGG GCTATTGAAA ATTTGG

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wmc(15g4) Plate reference : 15g4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 9 nov 2000 at 12:22
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No sequence 27.10.00.
PCR Comments :
No sequence available for this clone
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