Wheat microsatellite WMC 25/06/01

wmc(7f8) Plate reference : 7f8
WMC locus number : WMC Length of sequence : 324
Last updated : Tue, Aug 26, 1997 at 12:18 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [TC]3 TT [TC]2 TT [TC]7 Tom Primer 1 : GTCTC CATCT TTCTC ACAGA (45%) Tom Primer 2 : AAGAT TGTAT GTATG TGGAC (35%) EXPECTED PCR PRODUCT: 116 nts. No substantial microsatellite. Related to 6d10, 5h7, 5h12. Bases 82-324 (ie 3' side small microsatellite) match part of 5d6. Duplicate 6d10.
PCR Comments : 


Length of sequence : 324  Consensus sequence is :

..

TCTCACGTAT ATTACATGTC TCCATCTTTC TCACAGACCT AACTTCTTCC 
TCTCTCTTTC TCTTTCTCTC TCTCTCTCTG TCTCGTTAGG TTTGTCTCCA 
ACTCTCTTGT CCACATACAT ACAATCTTTC CACCCTATCT GCTCCATAGT 
TCAAGAAGCT CTCTCCTGCA CGTTCATTCA CACAATGGAT ATGTCAAAAT 
GTTGCTTCTA TAATGGCTGA TGTAGAGTTT TGGTTGTTTT TGTTGCATCC 
TACAAAGTAA TAACTATGAA ATGCATTTAG ATTCTCATTT GAGTGGGATT 
ATGTGAGTTT GAGCATGTTG TGAA
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wmc125(7g8) Plate reference : 7g8
WMC locus number : WMC125 Length of sequence : 530
Last updated : Tue, Feb 16, 1999 at 1:35 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [TA]5 TGC [GT]11 GA [GT]20 Tom Primer 1 : AGTTG ACTTC ATATG TTAGG (35%) Tom Primer 2 : TTATC GAAGG ACTAC GATAG (40%) EXPECTED PCR PRODUCT: 181 nts.(GT)11 213 to 234, (GT)20 237 to 276,
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (alleles 234-250).
Forward primer details :
ATAccAccATgcATgTggAAgT at 138 length 22 bases, Tm 63
Reverse primer details :
AccgcTTgTcATTTccTTcTgT at 367 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 251 bases.


Length of sequence : 530  Consensus sequence is :

..

AGTATTTATG ACTAGAACAA ACATCATTTG ACATCTAAAT CAACTGCAAA 
ACCATAGCTT AATAGAAGAT CGCATGCGAG GAATTCAAGA GAATTGGCTA 
GATTCTTTCT TGACCACGTC ATCAATAAAG CCGGAGAATA CCACCATGCA 
TGTGGAAGTT GACTTCATAT GTTAGGTATT GTAAGAATAT TACACATTGT 
ATATATATAT GCGTGTGTGT GTGTGTGTGT GTGTGAGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTAGCC AGTAGCCTCG GTCAGTGATG 
ACCATAAGGT TAGGGATCTA TCGTAGTCCT TCGATAAGTA AGTAAGAGTG 
TCGAACCCAA CGAGGAACAG AAGGAAATGA CAAGCGGTTT CAGTAAGGTT 
TTCTCTGCAA GCACAGAAAT TGTAGGTAAC AGATAGTTTT GTAATAAGAT 
AAATTGTAAC AGTAACAAGC AATGAAAGTA AATAAAGTGC AGCAAGGTGG 
CCCAATCCTT TTTGTAGCAA AGACAAGCCT
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wmc(7h8) Plate reference : 7h8
WMC locus number : WMC Length of sequence : 547
Last updated : Wed, Aug 20, 1997 at 9:47 am
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : 7H8 Microsatellite : [CT]16 Tom Primer 1 : CCATC TTTCT CACAG ACCTA (45%)Tom Primer 2 : GGACG AGATA GTAGG AGACA (50%) EXPECTED PCR PRODUCT: 89 nts. May be double insert, sequence 215-238 is related to linker. (GT)14 155 to 182, (CT)16 419 to 450. Second insert matches 6d10 etc. 3' side of microsatellite in second insert matches 5f7. No room for primers.
PCR Comments : 


Length of sequence : 547  Consensus sequence is :

..

CCGCTATCAA ACTACTCCGC TACTTACAAT TTCAGCACTT CGAGACATTA 
CCTTGCTGAA AACCACTTGA CATTTCCTTC TACTCCATTG GGTTTGACAC 
TCTTATTATC GAAAATAGCT ATAATTGATC CCCTATATAT TTGTGGGTCA 
TCAGGTGTGT GTGTGTGTGT GTGTGTGTGT GTGCGTCTGG GGGGTGGCGG 
GTGGCTGGCA GTTAGTCACG TAGTCCACGC GTCGACACAA ATTACCAATT 
CCTCTCTCCC GGCTCTTTTC CGTCAAATAC CTCAACCATG AGATCCTTCC 
CCTTTCATCC ATGTTTCCAG CTCTCTCATC AAACATGTTG TCTCTTCTCC 
TTCCAGCATA CAGAATCACG TATATTACAT GTCTACATCT CCATCTTTCT 
CACAGACCTA ACTTCTTCCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
TGTTAGGTTT GTCTCCTACT ATCTCGTCCA CATACATACA ATCTTTCCCG 
CCCTATCTGC TCCATCTTCA AAAGCTCTCT TTGCACGTTT CATTCAC
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wmc(7a9) Plate reference : 7a9
WMC locus number : WMC Length of sequence : 318
Last updated : Tue, Feb 4, 1997 at 21:56
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence : 318  Consensus sequence is :

..

GATATAACAT CCGAGTACAA GGTAGGAGAG ACGTGAGATT GAGAGATTGA 
GGCAGCGTGA CACCCACAGT ATACGTCGAT GCATGGAGTG TGTGATTGAA 
AGAGTTGCTC AAGCATTTGT GCGGTTTGTC GAGTTAGCCG AGAGCGTATG 
TGGGATGGTC GTAGTCGAGG TAGCCGTGCA AAGAACACCG TGGTCAAGTC 
GGGGTCGTCG TGGAGCTGCG AAGAAGAGCA GCGTGGCCTA AGGAGGCGGC 
GGCGGCAACT AGAGAAGGAG GCGGCGGCGC GTGCTCGAAG TAGGTGATAA 
CCGACAGCGT ACAAAGAA
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wmc126(7b9) Plate reference : 7b9
WMC locus number : WMC126 Length of sequence : 528
Last updated : Tue, Feb 16, 1999 at 1:35 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : 7B9 Microsatellite : [GT]~60 [AC]~30 COMBINATION. Size of microsatellite only approximate. (CA)28 257 to 312, (GT)60 136 to 255.
PCR Comments : 51C anneal, polymorphic on acrylamide (nulls for 188 base allele).
Forward primer details :
TTgTcAcAAAAgggTcgTAAAT at 48 length 22 bases, Tm 60
Reverse primer details :
ATcTcgAgATgTcATcgAAAAT at 362 length 59 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 336 bases.


Length of sequence : 528  Consensus sequence is :

..

GAGAAGGTGT TATAGTATTT ACGATCGCCA GGTTTTACCT TCTGTTTTTG 
TCACAAAAGG GTCGTAAATG AATTGTACCT TTCATGACCA ATAGTGTGTC 
AAGTTGACAT TTCTTGAACT CTTCCATATC NNNNNGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CACACACACA CACNNNNNGC ATCACACAGT ACACAGCTCA TCTCTGCCTA 
TCTCTCACTT CATTTTCGAT GACATCTCGA GATACAGTTC GATAAATGTC 
GAGTATCACT AGTTTACACA TGGATACTCC TGCATGGGCA ATGACAACAG 
GTGTTCTGTA AAAAATGCAT ATTATGTTCT GTGCAATGGC GAGAATCTTG 
CTTGTAATTA TATAACGCAA ACACAGCA
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wmc(7c9) Plate reference : 7c9
WMC locus number : WMC Length of sequence : 251
Last updated : Fri, Dec 5, 1997 at 2:25 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [TC]3 TA [TC]2 TA [TC]7 TA [TC]2 Tom Primer 1 : CATCT TTCTC ACAGA CCTAA (40%) Tom Primer 2 : ATATC CATTG TGTGA TGAAC (35%)EXPECTED PCR PRODUCT: 162 nts. Microsatellite like motif 20 - 62 with CT core. Close relative 7F9, unable to make unique primers to this sequence.
PCR Comments :
Expected size of amplified fragment (Chinese Spring=) 203 bases.


Length of sequence : 251  Consensus sequence is :

..

CATCTTTCTC ACAGACCTAA CTTCTTCCTC TCTCTATCTC TATCTCTCTC 
TCTCTCTATC TCGTTAAGGT TGTCTCAACT CTCTGTCAAC ATCATCAATC 
ATATCCTCCC ATCTGCTCCA ATAAGTCAAA GCTCTCTTGC ACGTTCATCA 
CACAATGGAT ATGTCAAATG TGCCCTATAA TGGCTGATGT AGAGTTTGGT 
TGTTCTCTTG CATCCTACAA AGTAATAACT ATGAAATGCA TTAGATTCTC 
A
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wmc(7d9) Plate reference : 7d9
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 4, 1997 at 21:56
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No sequence 15.1.97
PCR Comments :
No sequence available for this clone
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wmc(7e9) Plate reference : 7e9
WMC locus number : WMC Length of sequence : 347
Last updated : Jeu 27 Aož 1998 at 12:04
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No substantial microsatellite. Bases 30 to 65 show homology to 10g7 bases 69 to 104 (36 in longest run, maxScore = 49).
PCR Comments : 


Length of sequence : 347  Consensus sequence is :

..

CTTCTGTTTT TTCAATGATC TTCTTGTAAT TCACAGGAAA CCAGAAGTTG 
TGGAGGACGG TGCAGTCTTG GCAAGCCATG GCCAAGTTGT TGAGTATGGT 
GCGTGGCCGT TTGTGTGTGG GAAGTAGTCG AGCGCGAANN NNNNNNNNTG 
CTTTCGTTTC AACATCTCTG TGTCTAGAAC AGAGTGTGAG ACGTGCAGCC 
TTGGCGAACC ATGCCAAGTT GTTAGTATGT CGTGGCGTTT GTCGGCGGCG 
GCAAGCAGAT CGAGGCTGGC GAACGACGGC GTAGCGGGAA CAGCTCTGGT 
GCGATGGACG GTTGCGACAG TGGAGCGGCG GCAATGAGGG CGCAGAC
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wmc(7f9) Plate reference : 7f9
WMC locus number : WMC Length of sequence : 475
Last updated : Lun 5 juil 1999 at 17:23
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No substantial microsatellite. Bases 141 to 155 show homology to 26h11 bases 167 to 181 (complement) (15 in longest run, maxScore = 19).
PCR Comments : 


Length of sequence : 475  Consensus sequence is :

..

CACGTGAGGG CCCACAGGTG GGCTATCTTC CGGCAGCGCT ACACCAGCCA 
TTTTGCAGGC TCACATAATC TTCCTTTTCT CGTTCCTTTC CAAATCCTAG 
CCCTCCTTCT TCTTATTTGC GCTCAAATCT CGATGGCGCG AAGCATGACA 
GCGAGCTGGC ATGGCGCGCG CAGGCGGCAG AAGAGCGTTG CACCGTAGTT 
AGTGACCTGG TGCAGCAGTG GCGATTATGG CACAGAGCAC GCGCGATCTA 
GTGACAGGAC AGGTTAGTGG CGCGCGGCCG GAGAACCTCG GCTAGATTCT 
CAGCGCTACA CTTTGGTGGC AAAAAAGGTA AGGTCTTTAC CGGTTGGTTC 
TTTACATCCC TGCATTATCG CCCATAATTC ATCACCTGTG GCCATGACAA 
GCTCCATCTA TCTGGTCATC AGAAAACTCC TAGTGCCCGT GCGCATAGAT 
GCAGACAATT TTGGCAGCGC ACTTG
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wmc127(7g9) Plate reference : 7g9
WMC locus number : WMC127 Length of sequence : 325
Last updated : Tue, Feb 16, 1999 at 1:35 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [CA]9 Tom Primer 1 : AATCT CTATC AACTC CTCTT (35%). Tom Primer 2 : TTGAC AGATT AAGAT TAGAT (25%). EXPECTED PCR PRODUCT: 166 nts. Bases 173-255 (complement) have homology to 8c12(1). (CA)8 50 to 65, (CA)9 140 to 157.
PCR Comments : tested 61C anneal, no product; 51C anneal, high MW product.
Forward primer details :
TTgAcTAAcgggTTTAccTggc at 2 length 22 bases, Tm 64
Reverse primer details :
gTTgAccTTcTgcTgTcATTgc at 238 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 258 bases.


Length of sequence : 325  Consensus sequence is :

..

CTTGACTAAC GGGTTTACCT GGCTCCCACA CACACCCACA CACACATCCC 
ACACACACAC ACACACCTAC CCGAACACCG AAGGAAGTAA GAAGTTGGAC 
CTCGATCCTT GATAGTAATC TCTATCAACT CCTCTTCAAC ACACACACAC 
ACACACACTG CTAGTTGTGC CACTGTGCCT CTGTGCCTAC AGTGCACACT 
CTCGATACGA CTTATCTCCT CCCTCCCACC CACCCAAGCA ATGACAGCAG 
AAGGTCAACT CGATCTAATC TTAATCTGTC AATACCGACA GGCCGTCGTG 
GGTGCAGACT CCGCCCACTG TTGCT
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wmc(7h9) Plate reference : 7h9
WMC locus number : WMC Length of sequence : 134
Last updated : Tue, Feb 11, 1997 at 7:01 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : (CAA)26 3 to 80, (CAA)12 95 to 130. No room for primers.
PCR Comments : 


Length of sequence : 134  Consensus sequence is :

..

AACAACAACA ACAACAACAA CAACAACAAC AACAACAACA ACAACAACAA 
CAACAACAAC AACAACAACA ACAACAACAA AACAACAACC CTAACAACAA 
CAACAACAAC AACAACAACA ACAACAACAA CAGC
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wmc(7a10) Plate reference : 7a10
WMC locus number : WMC Length of sequence : ~1400
Last updated : Lun 5 juil 1999 at 17:23
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : 7A10 Microsatellite : [GT]4 [GA]2 TGGAGTCTTA [GT]7 GGGG [GT]6 [GA]8 G [GA]7 TGGAG TTCTT AGTTT C [GT]5 [GA]7 [GTC]6 NNN [GT]16 NN [GA]7 NNN [GT]8. Tom Primer 1 : CAAAT GCTCC GCCTA GAGCA (55%) Tom Primer 2 : BE NEXT CONFIRMED EXPECTED PCR PRODUCT: ?.(GA)8 160 to 175, (GT)16 267 to 298, (GT)8 318 to 333. Member odf 1g1 etc Bases 40 to 96 show homology to 26f9 bases 20 to 76 (57 in longest run, maxScore = 83). Duplicate with 26f9, 7a10 has priority.
PCR Comments : 


Length of sequence : ~1400  Consensus sequence is :

..

AGTAAAGTGG AATTTGTCCA TGTTATGCAA GTAAATAAAG GTGATTGTTT 
ATCATACGGG TAAGGTTGGA TGAAGGGTGG TAGGAACAAA TGCTCCGCCT 
AGAGCATGTG TGTGTGAGAT GGAGTCTTAG TGTGTGTGTG TGTGGGGGTG 
TGTGTGTGTG AGAGAGAGAG AGAGAGGAGA GAGAGAGAGA TGGAGTTCTT 
AGTTTCGTGT GTGTGTGAGA GAGAGAGAGA TGGAGTTCTT NNNNNGTCGT 
CGTCGTCGTC GTCNNNGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTNN 
GAGAGAGAGA GAGANNNGTG TGTGTGTGTG TGT
(break) 
NNNNNCGGTT 
CCAACGGCCA ACGGAACCTC GGCCTCGAAT TCGCCGCCTC CCGACGTTCG 
CCAGCTTCCC CGACGCGCCC ACAAGCTCCA CTGTGAGGCC CTCCTTCGAT 
TCCCCCTTTT CCCCCTCGTT TCCGTCACCG TAGTCGCCCG
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wmc(7b10) Plate reference : 7b10
WMC locus number : WMC Length of sequence : 411
Last updated : Tue, Feb 11, 1997 at 7:04 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [AC]17 Tom Primer 1 : GGGGT AGGTC GATGT GTCGG (65%) Tom Primer 2 : TGGTA ACATG CATGT CTTCC (45%) EXPECTED PCR PRODUCT: 137 nts(CA)16 73 to 104. Duplicate 7B7.
PCR Comments : 


Length of sequence : 411  Consensus sequence is :

..

TAACACTACT CACCTTCTCT GTGTAGGGGT AGGTCGATGT GTCGGTCTCT 
CTAACGCACA CATGCTGTTA AACACACACA CACACACACA CACACACACA 
CACACGGCCT GAGGTAAAAT GAGTCTATAT CTCAATTAAT GGAAGACATG 
CATGTTACCA TCCATATGTT ACTACCTACT ATGAAGTAGT AACACAAGCT 
AGTACATATG CATGTTACTA GTCCAAGGTT ACTCACCACT ATGACCAGCC 
TAGCAATGTA ACCAACACTC CTAGTACGCG TACTGTGGTG GACCATGGAC 
GAGCGCATGG GCCCACAACA ACATGTGGCG CATGTGTGTG TCCGACATGT 
AGCTCAATAC CCTCAGACGG ATAAGGGTAT ATCCAATGTT CATCCTCTAA 
ATTGGACCCA C
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wmc128(7c10) Plate reference : 7c10
WMC locus number : WMC128 Length of sequence : 291
Last updated : Tue, Feb 16, 1999 at 1:35 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [GT]16 [GA]10. Tom Primer 1 : CGAAT CGGAC AGCTA CTGCT (55%). Tom Primer 2 : CACCC TTGTG TGTCC ACGTT (55%). EXPECTED PCR PRODUCT: 134 nts. (GA)10 195 to 214, (GT)16 163 to 194. Bases 163-291 (ie. 3' side of microsatellite) homologous to part of 2g10 (reverse primer in this domain).
PCR Comments : 61C anneal, product (202 bases).
Forward primer details :
cggAcAgcTAcTgcTcTccTTA at 73 length 22 bases, Tm 63
Reverse primer details :
cTgTTgcTTgcTcTgcAcccTT at 250 length 22 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 199 bases.


Length of sequence : 291  Consensus sequence is :

..

ATGCTCTGGA CACTTCAACA ATGTGACCAT ACAGCTGCAA ACTCTGCATT 
CTGGGCCGTT GATTGGTCGA ATCGGACAGC TACTGCTCTC CTTATGTGGG 
ACAAGTTCAG AATTCAGCAC AGGCACAGGC TGGTAGCTCT GACACGTCAC 
ACGGTGACTC CAGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGAGAGA 
GAGAGAGAGA GAGAGGAGGG AGGCGCGATT AGATTTAACG TGGACACACA 
AGGGTGCAGA GCAAGCAACA GGATAAGTTT GATAGAATAG A
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wmc(7d10) Plate reference : 7d10
WMC locus number : WMC Length of sequence : 184
Last updated : Tue, Feb 11, 1997 at 7:05 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [GA]8 TA [GA]2 TAGAG GTA [GA]6. Tom Primer 1 : GCGAC AGAAC TGCCC CTTGT (60%). Tom Primer 2 : TACAC ACAAA CCTTG TTGGT (40%. )EXPECTED PCR PRODUCT: 85 nts. Trimmed to insert at 3' end. First 2 bases of Toms' reverse sequence were derived from linker. (GA)8 122 to 137. Matches 3d2, 5h6.
PCR Comments : 


Length of sequence : 184  Consensus sequence is :

..

CTGAAGATTA TCAGAGAGGA CAGAGAGAGA CAGAGAGAGA CAGAGAGAGA 
CAGACGAGAG AGAGAGAGAG GACTAACAAG GTTTGTGTGT TGGATGCGTG 
CGACAGAACT GCCCCTTGTC AGAGAGAGAG AGAGAGATAG AGATAGAGGT 
AGAGAGAGAG AGAGGGACCA ACAAGGTTTG TGTG
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wmc(7e10) Plate reference : 7e10
WMC locus number : WMC Length of sequence : 135
Last updated : Tue, Feb 11, 1997 at 7:06 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No room for primers. (TTG)44 2 to 133.
PCR Comments : 


Length of sequence : 135  Consensus sequence is :

..

GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT 
TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG 
TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTT
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wmc(7f10) Plate reference : 7f10
WMC locus number : WMC Length of sequence : 302
Last updated : Tue, Feb 4, 1997 at 21:56
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [TG]2 [TC]3 GA [GCC]5Tom Primer 1 : TGATG CGATC TTGGT GCATC (50%)Tom Primer 2 : GTGGC GGTCG AAGTA TAGGA (55%). EXPECTED PCR PRODUCT: 117 nts. No substantial microsatellite.
PCR Comments : 


Length of sequence : 302  Consensus sequence is :

..

GTGCCGTTGG TGTTGGAACT GCTATAGAAG GACCTAATTG CCGGTTCCGT 
TGTTGCCGCC AACCGAAGTG TGTGCCTTCC CAGTTGTTCA ACTCGGTAAC 
GAGTTCCGGC CTGCGTTGGT TCCTCTTCTG CTGATGTATG TGAAGGTCTT 
CTTGATGCGA TCTTGGTGCA TCATACGCAA AGGCCTTGTG TGTGCTGTCA 
TCGTCGTCGA GCTCGAGCGT GTGTCTCTCG AGCCGCCGCC GCCGCCACAT 
CCTATACTTC GACCGCCACG AGCAAACCAC TAGCCCAACA TCCCGCCGCT 
GC
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wmc(7g10) Plate reference : 7g10
WMC locus number : WMC Length of sequence : 244
Last updated : Tue, Feb 4, 1997 at 21:56
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [TG]6 GC [GT]2 [GA]2 TG [GA]3 GGAAC AAG [GA]3. Tom Primer 1 : ATACT TGGCT AGCTA GGCAT (45%). Tom Primer 2 : CTCGT CTCCT TCCCC GAGCC (70%). EXPECTED PCR PRODUCT: 159 nts. No substantial microsatellite.
PCR Comments : 


Length of sequence : 244  Consensus sequence is :

..

GACCTACATG TGCCTACAAT TGACTGAGAG ACCATCATAC TTGGCTAGCT 
AGGCATGATA TTTGTGTGTG TGTGGCGTGT GAGATGGAGA GAGGAACAAG 
GAGAGAGTTT AGATGGCCTA TCGAGTGCGA GGTCAATTCA ACTCAGTCTC 
GGTTGTCGGT CGGCGCGCTA GGTCCGGCTC GGGGAAGGAG ACGAGCAGGG 
TAGTCCCAGC AGCTCTTACT TCTCTTGCAA GGCTCCTTCC TAAC
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wmc(7h10) Plate reference : 7h10
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 11, 1997 at 7:07 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No room for Primers. (CA)18 71 to 106.
PCR Comments : 


Length of sequence :   Consensus sequence is :

..

NNNNNNTGAT TTGCGCGTAC ACTCATCTCT ATATGAACGC ATGCACACTT 
GGGTTACATC TGCCTCCGAG CACACACACA CACACACACA CACACACACA 
CACACA
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wmc(7a11) Plate reference : 7a11
WMC locus number : WMC Length of sequence : 307
Last updated : Thu, Aug 21, 1997 at 2:48 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [GTGC]3 [GT]5. Tom Primer 1 : ATCTC TACCA CCGAC TTGTA (45%). Tom Primer 2 : CGAGT CGATG GTCAA CTTGC (55%). EXPECTED PCR PRODUCT: 149 nts. Duplicate of 7e11, 5b5(1), related to 11H3, 2h10. (GT)8 35 to 50. No room for primers.
PCR Comments : 


Length of sequence : 307  Consensus sequence is :

..

AAGGCATGGC ATACGTGTGT GTGCGTGCGT GTGCGTGTGT GTGTGTGTGT 
GGTTTTGTTT TGTCATGGTC CTAGTTGCAT GTCAATAATC GACTCGCAAC 
TAGAAGTGCG TGCGTGTGTG TGTCTTTTTG TCAAGGGCCC CGGTTGCTAT 
CTCTACCACC GACTTGTAAC TAGGGGGTGC GTGTGTTGAG GACCCCTAGT 
TGCAAGTTGT CCATCGACTC GCAACTAAGG GATGTGTGCG TGCGTGCGTG 
TGTGTGTTTT ATTCGTGGCC CCCAGTTGCA AGTTGACCAT CGACTCGCAA 
CTAGGGG
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wmc(7b11) Plate reference : 7b11
WMC locus number : WMC Length of sequence : 706
Last updated : Tue, Feb 4, 1997 at 21:56
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [GT]4 GC [GT]7 TTT [GA]4. Tom Primer 1 : AATTA TAGAT ACGAC GGAGA (35%). Tom Primer 2 : GATAC TAATC TATCG ACTGG (40%). EXPECTED PCR PRODUCT: 106 nts. Bases 136-275 (ie. 5' side and 3' side of microsatellites) homologous to 2g11. Too difficult to design primers, sequence 3' of small microsatellite (275-287) is duplicated 5' of large microsatellite (176-200).
PCR Comments : 


Length of sequence : 706  Consensus sequence is :

..

CAAATATAAA ATATGCAACA AACAGGAACT CGCACTGGGC ACTGGATCAA 
TATGTTAGGT CCAAAAATAG TATGAAAAGT TGCCAAAAGT GTATGAAAGT 
TGTAGAATAT TGGCATGGAA CGATCAAAAA TTATAGATAC GACGGAGAGT 
ATCACCTAGT GCCTTCGGTG TCGGTAGTGT GTGTGCGTGT GTGTGTGTGT 
TTTGAGAGAG AAGGCCAGTC GATAGATTAG TATCAAGATC GAGGTGTCAC 
CCTTACTCCT TCGGTGTTGG GAAGGTGTGT GTGTGTGGTT GTGTGGTAGG 
TCCAAGCTGA CAAATATAAA ATATGCAACA AACAGGAACT CGCACTGGGC 
ACTGGATCAA TATGTTAGGT CCAAAAATAG TATGAAAAGT TGCCAAAAGT 
GTATGAAAGT TGTAGAATAT TGGCATGGAA CGATCNNN
(break)
NNNNN 
TGCACACGCC TACCTTACTC GGCTGCGTCT AGGGCTATGC TGCTGCTCCC 
TGCACGCGCT CTGGCCTGCC TGCTGCGAGC TCTGATGGGA TCGCCGAAAG 
AGGAGATCCA GGCGAATCCT GCGGTCCGTC GGAGGTGCCG GCGACCCCTC 
TCATCCGTCG CCCGGGTTCC CCGAGGCGGG ATCAAAGGTG CAGGCTTTGG 
ACCGGCGGTT GGCGGCGTGG CTCCCCCATC TGAGCGCCCC CGCCTAAAAT 
CCATTGTGGT AAG
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wmc(7c11) Plate reference : 7c11
WMC locus number : WMC Length of sequence : 217
Last updated : Tue, Feb 4, 1997 at 21:56
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence : 217  Consensus sequence is :

..

AAGAGGTGCT CCTACTTGGT GGTGGTCAAA TCCTCATCCC TAGGGACCCA 
TATCCAGTTG GGGATTGAGC ACCGCTGGCC GACAATCGCC GGAGGAGGTA 
TCAGCCGCGC CGCTCCGCTG GCCGTCAGCC AGCACGGAAT GTCGGAATAG 
GGCTGCCATT TAGCGGGACA ATCATCAAAG AGGAATCACA CACAGGCGCC 
CGCGTCGCCG CTCGCCG
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wmc(7d11) Plate reference : 7d11
WMC locus number : WMC Length of sequence : 219
Last updated : Jeu 27 Aož 1998 at 12:07
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [GA]3 CG [CA]8 [CT]7. Tom Primer 1 : TCTGT TATCA TTGCC TCAGT (40%). Tom Primer 2 : AAACC CTTGT TGAGC GTGTT (45%). EXPECTED PCR PRODUCT: 95 nts.(CA)8 80 to 95. Duplicate of 1D9. Bases 96 to 141 show homology to 10f12 bases 114 to 159 (46 in longest run, maxScore = 67).
PCR Comments : 


Length of sequence : 219  Consensus sequence is :

..

ACATGGTCTC TCTCTATCCT CCACACACAG ATACCCCGAA TCTGTTATCA 
TTGCCTCAGT CTCTAACATA TCACACACGC ACACACACAC ACACACTCTC 
TCTCTCTCTC GCAAACACGC TCAACAAGGG TTTCCCCGTG AGTAACTTAC 
CGTCTATTCA TCAACAGTCG TGGCAGTACG GCGAACATGA GACGTGAAAA 
GGAATAAATT CACACGTGC
Go back to index
wmc(7e11) Plate reference : 7e11
WMC locus number : WMC Length of sequence : 307
Last updated : Tue, Feb 11, 1997 at 7:11 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [GTGC]3 [GT]5 . Tom Primer 1 : ATCTC TACCA CCGAC TTGTA (45%). Tom Primer 2 : CGAGT CGATG GTCAA CTTGC (55%). EXPECTED PCR PRODUCT: 149 nts. Duplicate of 7a11 (GT)8 35 to 50.
PCR Comments : 


Length of sequence : 307  Consensus sequence is :

..

AAGGCATGGC ATACGTGTGT GTGCGTGCGT GTGCGTGTGT GTGTGTGTGT 
GGTTTTGTTT TGTCATGGTC CTAGTTGCAT GTCAATAATC GACTCGCAAC 
TAGAAGTGCG TGCGTGTGTG TGTCTTTTTG TCAAGGGCCC CGGTTGCTAT 
CTCTACCACC GACTTGTAAC TAGGGGGTGC GTGTGTTGAG GACCCCTAGT 
TGCAAGTTGT CCATCGACTC GCAACTAAGG GATGTGTGCG TGCGTGCGTG 
TGTGTGTTTT ATTCGTGGCC CCCAGTTGCA AGTTGACCAT CGACTCGCAA 
CTAGGGG
Go back to index
wmc129(7f11) Plate reference : 7f11
WMC locus number : WMC129 Length of sequence : 217
Last updated : Tue, Feb 16, 1999 at 1:35 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [CA]12. Tom Primer 1: TAACG TGGGC TGGCT AGGGT (60%). Tom Primer 2: CTACG TCTCG GCGCC CGGAG (75%)EXPECTED PCR PRODUCT: 150 nts.(CA)12 111 to 134, bases 110-217 3' side of microsatellite match 2a10. Bases 138-205 (ie 3' side of microsatellite) match part of 11f10 (complement), reverse primer is in this duplicated region.
PCR Comments : 61C anneal, product (78, 150 and 290 bases)
Forward primer details :
TgAgAgccgAccTccTcTTAcT at 61 length 22 bases, Tm 63
Reverse primer details :
cggAgAcggTcgAcTggATTAT at 134 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 95 bases.


Length of sequence : 217  Consensus sequence is :

..

AACGTTGCAC GTGTGTAGCT AACGTGGGCT GGCTAGGGTC TTGGCCGCAT 
CTTCGTATGA TGAGAGCCGA CCTCCTCTTA CTGGTCACAA GGGTTCACAC 
GCCCTATAGG CACACACACA CACACACACA CACATAATCC AGTCGACCGT 
CTCCGGGCGC CGAGACGTAG GGCTATTACT TCCTCGGCGA AGGGCCTGAA 
CTCGTAAAAC TCGTGTG
Go back to index
wmc130(7g11) Plate reference : 7g11
WMC locus number : WMC130 Length of sequence : 624
Last updated : Tue, Feb 16, 1999 at 1:34 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [GT]32. Tom Primer 1 : GAGGA GAGAT AGAAC AGATC (45%). Tom Primer 2 : TTGTC CATTG GTTCA CAAAC (40%)EXPECTED PCR PRODUCT: 136 nts.(GT)32 384 to 447.
PCR Comments : tested 61C anneal, no product; tested 51C anneal, no product.
Forward primer details :
TgTggTgccAAcTTcTTcTATg at 281 length 22 bases, Tm 62
Reverse primer details :
cTccgTgAcTTcTcTcTcAAAA at 498 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 239 bases.


Length of sequence : 624  Consensus sequence is :

..

GTTTGGTAAA CAGGCTTCCT GTGACACGGC AGAAGCGGAC GATGTGACCG 
GATCGAAGAC AGTCCATGAC ATCGGATAGG ATCCATTCTT GAGACGAGCT 
GCCAGCCCTG CAACACATTC CGCATGGAGC GTGACCACTA GGAATTGCCG 
GTCTGCTCTC GGTCGAGAAC ATCCTTGCCC AATGTCCACA GGACAGGAAG 
CACAGGAGGT CCACCTTTTG TCGGTCCCGC ACACACTCGC ATCAGGCTCC 
ACACACTCCA CAGAGGCAGC CCGCGGCTNT TGTGGTGCCA ACTTCTTCTA 
TGTGTGTGTG TGTGCATAGC GGCCATTCAA CCCAGAGGAG AGATAGAACA 
GATCTCACAT GNCTTTCTAG AGACTCAGGA GAAGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTCGT 
GTTTGTGAAC CAATGGACAA ACCCAACATG TGGTGCTCTC TCACAACTTT 
TGAGAGAGAA GTCACGGAGA GAGGACCAGA TAGAAGGCCA GCAGCAGCTT 
GCAACAATCG TTGATGAGTC TAATAGCACT AGTAGTTACA ACGGCGCATC 
TAGAAGGCCA CCGCCTGCGT GCAG
Go back to index
wmc131(7h11) Plate reference : 7h11
WMC locus number : WMC131 Length of sequence :
Last updated : Tue, Feb 16, 1999 at 1:34 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite shown here is (AT)10(GA)10, in the original sequence file was marked [AT]N[GA]N. (GA)10 80 to 99, (TA)9 61 to 78, Bases 285 to 317 show homology to 12a2 bases 207 to 239 (33 in longest run, maxScore = 28).
PCR Comments : tested 61C anneal, no product.
Forward primer details :
gcAAcgTcATgcTccgATAAc at 30 length 21 bases, Tm 65
Reverse primer details :
gcATccAgTccTTcTTcgcTT at 200 length 21 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 191 bases.


Length of sequence :   Consensus sequence is :

..

GAGCGCTCTA TTACTTCNNN NNNNNNNGCG CAACGTCATG CTCCGATAAC 
CGAANNNNNA TATATATATA TATATATATG AGAGAGAGAG AGAGAGAGAN 
NNNNCTAGGC GTGGAAGCAG ATCTAGGGTA GACGGTGGCG GCGGGCGCGG 
TAGCGAGATG GTCGTAGGAG CGGNGCCGGC AAGGCGCGAC GGCGGGATGA 
AGCGAAGAAG GACTGGATGC AAGGATCCCG GCAAGGTGCA CACGGCGGGA 
TGAAGCGAGA GGACTGGATG CAAGGATCCC GGTCCCGAAG CCGTGGATGA 
GAGAGGTCGA TCTCTTGCAA TGGATGGCGG CGGCGTCGGG GTATCAGCTC 
TGGCAGGTGG AGGAGGTCGG CGGAGGATGG GGGCGGCGGA CNNGTTTTTC 
CCAGTCACGA CGTTGTAAAA CACGGCCAGT GACCCAGTCA CGACGTTGTA 
AAACACGGCC AGTGA
Go back to index
wmc132(7a12) Plate reference : 7a12
WMC locus number : WMC132 Length of sequence :
Last updated : Tue, Feb 16, 1999 at 1:34 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Tom Primer 1 : GCCTA GATGA CTCCA CGAAC (55%) (GT)34 276 to 343, (CT)32 401 to 464. Expected fragment size is estimate.
PCR Comments : tested 61C anneal, no product.
Forward primer details :
cTAggTcAcATTgcggcgAAgT at 235 length 22 bases, Tm 67
Reverse primer details :
gAcggccAggAggATTATcAgT at 379 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 166 bases.


Length of sequence :   Consensus sequence is :

..

ATAAGTTTGA GAAAAACCGC ATGAGTGTGC ACTCCACCTC AACAGGTAAT 
TAAGGCCGAT GAATGCACTT ATGGTTGGGA CACACACGCG GCAGGCTACC 
AACCGCTGCC TCATGACCAT ACTATCAAGG AAGACGACAA TGAGAACACG 
AAAGAGCAAG AGAGGACTAA GCAAACCAAC CTTGATCTCA TGATTACCAT 
CACCTTACAG TTGAAGCCTA GATGACTCCA CGAACTAGGT CACATTGCGG 
CGAAGTCATG ATNNNNNNNN NNNNNGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTNNNNNAC 
GTCCTCTTGC TTGCTTGCTG TGGAAGGAAC TGATAATCCT CCTGGCCGTC 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTCTCTCTCT CTCTCAACAT GT
Go back to index
wmc133(7b12) Plate reference : 7b12
WMC locus number : WMC133 Length of sequence : 234
Last updated : Tue, Feb 16, 1999 at 1:34 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [AT]2 AC [TC]4 [AC]9 CAG [AC][AC]9. Tom Primer 1 : TCTCG ATCTC ATCGA GAAGG (50%). Tom Primer 2 : GCTCA AGTCA CCGCG TCAAC (60%). EXPECTED PCR PRODUCT: 152 nts.(CA)9 55 to 72, (CA)9 120 to 137.
PCR Comments : tested 61C anneal, no product.
Forward primer details :
cATcTcgATcTcATcgAgAAgg at 16 length 22 bases, Tm 63
Reverse primer details :
AccgcgTcAAcTAcTTAAgccA at 139 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 145 bases.


Length of sequence : 234  Consensus sequence is :

..

ATGAATTAAG TAGAGCATCT CGATCTCATC GAGAAGGATG GATATACTCT 
CTCTCACACA CACACACACA CACCAGACAC AAGTTGGCCC GGCCTTCCAG 
CCATTGGTTC TGCCCACCTC ACACACACAC ACACACACTG GCTTAAGTAG 
TTGACGCGGT GACTTGAGCC ATTCTGGCCC AGCAAGCTGG CGGGCTGTTG 
GGGCTTGCGA TGGGGCCTGG ACGAGGTGGG TTAG
Go back to index
wmc134(7c12) Plate reference : 7c12
WMC locus number : WMC134 Length of sequence : 234
Last updated : Tue, Feb 16, 1999 at 1:34 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite : [TG]18. Tom Primer 1 : CCAAG CTGTC TGACT GCCAT (55%). Tom Primer 2 : TATAG ACCTC TGGCT CACGG (55%). TOM's EXPECTED PCR PRODUCT: 135 nts(GT)17 161 to 194.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (152-174 bases).
Forward primer details :
ccAAgcTgTcTgAcTgccATAg at 86 length 22 bases, Tm 64
Reverse primer details :
AgTATAgAccTcTggcTcAcgg at 201 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 137 bases.


Length of sequence : 234  Consensus sequence is :

..

GCTCGAGCTT CGATAGCAGC TATCTGGATC AAGATGGTAT AAGACGCTCG 
TTCTGAGATG GAGAGTGTCA CACTAGCTAC ACAGACCAAG CTGTCTGACT 
GCCATAGGCC TGAGCCATGG AGTGGTGTGT GGTGACGACT AGTGGGTCCC 
TCTGTTAGAT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGAGTTG 
CCGTGAGCCA GAGGTCTATA CTTAACTCTG TAGT
Go back to index
wmc(7d12) Plate reference : 7d12
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 4, 1997 at 21:57
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence :   Consensus sequence is :

..

AACAAAAACA AACTGGTTCT TATATCAACT ACACACTATC NNNACCTGCT 
ACCAGAAAGG TATGGCTCGG AATTGATTTA CACACATGCA TGATTCTTCA 
TTACATCTGT GCATGCATCC TTGAATCGTA ATTGACTGCT TTCATGCAAG 
CTTCCGTGTT GGAAGACACT CGTAGCTATG GCCGCCGCCG CCGTCGTGTT 
AACTCAACGC GCGCTAACGA CCGCTTGGCA GTCGCTACAG CCGCCGCCGC 
CTAGTCATCA TGNNN
Go back to index
wmc(7e12) Plate reference : 7e12
WMC locus number : WMC Length of sequence : 286
Last updated : Tue, Feb 4, 1997 at 21:57
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Tom Primer 1 : GCGAC TCCTC CTCCG ACTCC (70%). Tom Primer 2 : CGATC TAGGG TTTGA GGGCC (60%). EXPECTED PCR PRODUCT: 180 nts. No substantial microsatellite.
PCR Comments : 


Length of sequence : 286  Consensus sequence is :

..

GCTCAGAAAC GCCCTCTCTC GCGACTCCTC CTCCGACTCC CTGAAGCAGC 
CGGCCGGCGC CGGCTCTGGT CAGTTCTCCG GCCCTCCTCT CCCTCTCCCA 
TCCGTCCCTC CCTCCTCTTC CTCCCATCCG TCCCTCCTCC TCTTACATCT 
CTTGTCCGGC CATGGCGCGA CCTCCTCCAG TGAGGCCCTC AAACCCTAGA 
TCGTATCGGG ATTTGCGGCG GGATTGGGGG TCCGCAGGGA ACGGCGGCAG 
TAGTGGAACG ACGGCGGTAT TGGCTCCTTC TCTAGT
Go back to index
wmc(7f12) Plate reference : 7f12
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 11, 1997 at 7:19 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : (CA)19 24 to 61, (CT)11 2 to 23. No room for primers.
PCR Comments : 


Length of sequence :   Consensus sequence is :

..

TCTCTCTCTC TCTCTCTCTC TCTCACACAC ACACACACAC ACACACACAC 
ACACACACAC AC
Go back to index
wmc(7g12) Plate reference : 7g12
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 4, 1997 at 21:57
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence :   Consensus sequence is :

..

ACTAGCATCA TGTTGGCACA TGCCATAATG TAGGCGTAGA ACCACACAAT 
GTCTACTAAG AGCGTGTAAC TGCATGTCCT CGTACAGTTC ATAGGGCCAA 
CNNNNN
(break)
NNNNNTC GAAGGCGCGT TGGAACTACT TCTGCCTACT 
TCGAGCACGC CGCCGCACTC CTAACCTAGC CGCCGCCACC ACCACCTTCT 
AAGGCCACTG CTGCTGCTCT ACTTCCTCGG GCCACTGCCG CTCGCTCACT 
CGAGCCGCAC TGCCCATCCA CCCCCTTCGT CTTTGTCCAG CATCAACTCA 
TTGACAACAT CGTCATCATC TTCCTCGGAG CAGTCGTTAC CCGACCAGTC 
GTTGGCATAT CCAAGCCCAT TATCGATTGG TGCCGCTACC GTCGTCGAGT 
CCTTTTCTAC TGGCCCCTCT GACTCCCCCG ATATGTATAC AGCCTGTGCA 
TGTCCCCCTC TATGTATGCA TGGAGCTGGC AATACTGAC
Go back to index
wmc135(7h12) Plate reference : 7h12
WMC locus number : WMC135 Length of sequence : 240
Last updated : Tue, Feb 16, 1999 at 1:34 pm
Owner : Blake, Montana Date at Agrogene : 15.1.97 Public Domain : Yes
Comments : Microsatellite: (AC)~30. Tom Primer 1 : GAAGC TATAC AGTTA TCATC (35%) Tom Primer 2 : GGATG GGGAT GAATT GTGTG (50%) EXPECTED PCR PRODUCT : ABT. 170 nts. Size of microsatellite is approximate.(CA)29 95 to 152. Bases 190-232 homologous to part of 7a2. May expect secondary fragment of 77 bases.
PCR Comments : 61C anneal, product (slightly stuttery) at 80 bases
Forward primer details :
cAAccATcAAcAgccAcAcAgc at 14 length 22 bases, Tm 68
Reverse primer details :
gccATATAgccTTggATggggA at 202 length 22 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 210 bases.


Length of sequence : 240  Consensus sequence is :

..

AACGTGACAC ATACAACCAT CAACAGCCAC ACAGCACAGA AGCTATACAG 
TTATCATCAG CCCTTCGACT AGGGAGGAAC TAGATNNNNN NNNACACACA 
CACACACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CACNNNNNNN NNNNNGCAGC ACACACTTCC CCTCCCTCAG CACACAATTC 
ATCCCCATCC AAGGCTATAT GGCGACCACT CTGCTTGTGC
Go back to index
wmc136(5A7) Plate reference : 5A7
WMC locus number : WMC136 Length of sequence : 216
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Microsatellite motif consists of (AGGCGG)4 at 68-96. Several products one of correct size.
PCR Comments : 61C anneal, product;
Forward primer details :
gTATgATgAcAcATggTgcTgc at 12 length 22 bases, Tm 63
Reverse primer details :
gAccTgATTcTgcTgTgcTcTg at 188 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 198 bases.


Length of sequence : 216  Consensus sequence is :

..

ATTAGTAGAC AGTATGATGA CACATGGTGC TGCTCTATGA GCAGGAGCTG 
CCCGCTTGCT CGCTGGCAGG CGGAGGCGGA GGCGGAGGCG GAGGCGATCA 
GATCTCATAG CTCCACGTGC TCTCCGCTAC GAGCTTCGGT GCTTTGCTTT 
GATCGGACAG ATCGAACGTC GTCGCAACCT GCACAGACAG AGCACAGCAG 
AATCAGGTCA AAAAGC
Go back to index
wmc(5b7) Plate reference : 5b7
WMC locus number : WMC Length of sequence : 318
Last updated : Thu, Aug 21, 1997 at 3:18 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite. Contains some sequence related to 1e12. Bases 1-191 match complement of part of 5a6.
PCR Comments : 


Length of sequence : 318  Consensus sequence is :

..

GTCAATCATC GGGCGGTGGG CGGTGGCGGC ATCATAGGAG GTCAATCCTC 
GGTCTGCGGC GGGAGATAGG AGGAGCTCAA CCATCGAGGT CGGCGGCTGC 
GTGATTCGAG CACATCCATT GCGGTCGATT GCGGGATAGT GGAGGTCAAA 
CTTCAGGCGG CAGCGGCACT AAGCTTTGCT CCTCGACCCT GCTGTCTCGG 
CGTGTCTCCG CATCGCGCTT CTTTGTCTGC TGGATGGAGG TCGCCGCGCG 
GCTAATTAAT TAAGGTATTC TTTTCGTGAG TGTCTTAATT AAGGTATTCA 
ATTCCTAAGG GTAGTGTT
Go back to index
wmc137(5c7) Plate reference : 5c7
WMC locus number : WMC137 Length of sequence : 322
Last updated : Wed, Aug 27, 1997 at 8:35 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)16 180 to 211. No product of right size - large fragement 340 bases may be polymorphic. Redesign primers? Bases 247-303 match 8c12(1) bases 1-53. Bases 1-209 (ie. 5' side of microsatellite) match part of 13e2.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cgAgAAgTcTAcATATcgAggg at 97 length 22 bases, Tm 60
Reverse primer details :
cAAcAATgAcAAcAgAAgggTg at 265 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 190 bases.


Length of sequence : 322  Consensus sequence is :

..

TTAATTAAAA TAAAACAGGG TGAAAACTAT CACATGAGGC CGGTAAAAAC 
AAGGCACACT GGGTTCAGCA TCATCGTCAC TACAACTGTG CGCGCACGAG 
AAGTCTACAT ATCGAGGGGG CTACGGAGAC AAAGATGTAT CGAGTGTGTG 
TGTGTGTGCG AGGCACAGAG CCACAACTAG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGAGAGACAG AGAGAGAGAA GCCAATTGAT AGATTAAGAT 
CAAGATCGAG TTGTCACCCT TCTGTTGTCA TTGTTGGGGG TTTGGGGATG 
GGGGAGAGAG AGAAAGACAT AT
Go back to index
wmc(5d7) Plate reference : 5d7
WMC locus number : WMC Length of sequence : 208
Last updated : Tue, Feb 11, 1997 at 1:04 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence : 208  Consensus sequence is :

..

AAACCAATGA GCGAAGTCAG GTGGCCCCCC GAAACAGATC CACCTCCTCA 
GCGTCGCGTG GTGGGAAGAC GAGAACCTCC CAAAGGCCAA AGCCCATAAC 
CCCTAACCTA GGGTTTCGCC GCCATGGGAT CCGCCGCCTC CGCCGCCTCC 
GACGCAGNCG CCGCCACCGT CCTTTCTCCA GCGNCAACCA CACGGGGACC 
GCGCCGCC
Go back to index
wmc(5e7) Plate reference : 5e7
WMC locus number : WMC Length of sequence : 309
Last updated : Tue, Feb 11, 1997 at 1:05 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence : 309  Consensus sequence is :

..

TACTGATCGG CGGATTGAGC ACCGATAAGT GGATTTATGA CAGGTGGGAC 
CCGTTTTTTG CCGACGTGGC GCTGTCGACT GGTCGGACAA CCGTTAGATG 
CCGTTAGAAC GCCGCTCTCG CACGTGAACT TAAGTGATTC GCTCCCCTGT 
CTCTCAGTCT CACTCTCACT TTGCTCTCGC ACGCTCTCGC TCTCGCTCTG 
CTCTTGCTCG CCGCCGCCGG CCGCTCTGCT CCGCGTCGTG CCGCCACCGC 
CGCCGCATCG TCATGGTGTC CTGGAGTGAA AGAGACGATA GCAGCGAGCA 
CACTAGGCA
Go back to index
wmc138(5f7) Plate reference : 5f7
WMC locus number : WMC138 Length of sequence : 320
Last updated : Wed, Aug 20, 1997 at 9:49 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)14 172 to 199, (GA)8 200 to 215. Bases 80-180 (complement) ie 5' side of microsatellite match 3' side microsatellite in 7H8.
PCR Comments : 51C anneal, product;
Forward primer details :
AATcccggTcAAATgAgAAT at 2 length 20 bases, Tm 60
Reverse primer details :
gAgAcATgcATgAATcAgAA at 300 length 20 bases, Tm 57
Expected size of amplified fragment (Chinese Spring=) 318 bases.


Length of sequence : 320  Consensus sequence is :

..

TAATCCCGGT CAAATGAGAA TCTAAATGCA TTTCATAGTT ATTACTTTGT 
AGGATGCAAA AAAAACAACC AAAGCTCTAA ACGTGCAGAG AGAGCTTTTG 
AAGATGGAGC AGATAGGGCG GGAAAGATTG TACGTATGTG GACGAGAGAG 
TAGGAGACAA ACCTAACGAG ACACACACAC ACACACACAC ACACACACAG 
AGAGAGAGAG AGAGAGGGAG AGAGAGAGAG GAAGAAGTTA GGTCTGTGAG 
AAAGATGGAG ACATGTAATA TACGTGATTC TGTAGAGTAT TCTAATAAAT 
TCTGATTCAT GCATGTCTCT
Go back to index
wmc(5g7) Plate reference : 5g7
WMC locus number : WMC Length of sequence : 0
Last updated : Tue, Feb 11, 1997 at 11:27 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No insert
PCR Comments :
No sequence available for this clone
Go back to index
wmc(5h7) Plate reference : 5h7
WMC locus number : WMC Length of sequence : 320
Last updated : Lun 30 oct 2000 at 16:59
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Matches 6d10, 5h12, related 7f8. Small interpsersed microsatellites. Bases 229 to 256 show homology to 26f3 bases 419 to 446 (28 in longest run, maxScore = 137). Bases 166 to 185 show homology to TW6h09.2 bases 7 to 26 (complement) (20 in longest run, maxScore = 53).
PCR Comments : 


Length of sequence : 320  Consensus sequence is :

..

TATGTTACAT GTCTCCATCT TTCTCACAGA CCTAACTTCT TCCTCTCTCT 
TTCTCTCTCT ATCTCCCTCC TTCTGTTTCG TTAGGTTTGT CTCCTACTCT 
CTCGTCCACA TACATACAAT CTTTCCCGCC CTATCTGCTC CATCTGCGCA 
AGCTCTCTCT GCACGTTTCA TTCACACATT GGGATATGTT CAAAATGTTG 
CTTTTATAAT GGCTGATGTA GAGTTATTGT TGGTTTTGTT GCATCCTACA 
AAGTAATAAC TATGAAATGC ATTTTGATTG TCATTTGACT GGGACTATGT 
GAGTTTGAGC ATGTTGTGAA
Go back to index
wmc139(5a8) Plate reference : 5a8
WMC locus number : WMC139 Length of sequence : 194
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)12 144 to 167.
PCR Comments : 51C anneal, smear;
Forward primer details :
TgTAAcTgAgggccATgAAT at 121 length 20 bases, Tm 60
Reverse primer details :
cATcgAcTcAcAAcTAgggT at 57 length 20 bases, Tm 57
Expected size of amplified fragment (Chinese Spring=) 66 bases.


Length of sequence : 194  Consensus sequence is :

..

CCCCTTGATN AAAAGACACA CACACACACA CGCCCCTAGT TGCAAGTCGG 
TTATCGACAT GAAATTGGGG ACCCTTGACA CACACACACA CATCCCCTAG 
TTGCGAGTTG ATGTTCAACT TGTAACTGAG GGCCATGAAT AAACACACAC 
ACACACACAC ACACACACCC TAGTTGTGAG TCGATGGGCA ACTT
Go back to index
wmc(5b8) Plate reference : 5b8
WMC locus number : WMC Length of sequence : 413
Last updated : Mar 20 Juil 1999 at 13:59
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)12 193 to 216. Repeated structure 3' to microsatellite makes it too difficult to design primers. Bases 286-375 match 2g11 3' side of microsatellite, bases 271-392 ie 3' side of microsatellite match complement of 9b1. Bases 306 to 338 show homology to 19e4 bases 74 to 106 (33 in longest run, maxScore = 53). Bases 293 to 386 show homology to 10e11 bases 298 to 391 (94 in longest run, maxScore = 145), duplicate with 10e11, 5b8 has precedence. Bases 299 to 318 show homology to 21g7 bases 84 to 103 (complement) (20 in longest run, maxScore = 73). Bases 266 to 413 show homology to 20f8 bases 78 to 225 (148 in longest run, maxScore = 115). Duplicate with 20f8, 5b8 has priority. Bases 89 to 140 show homology to 10d2.1 bases 251 to 302 (complement) (52 in longest run, maxScore = 82). Duplicate with 10d2.1, 5b8 has priority.
PCR Comments : 


Length of sequence : 413  Consensus sequence is :

..

CAGCTGTGCG CGCGTGAGAA GTCTACATAT CGAGGGGGCT ACCGAGCTAG 
GCACCGAAAC ACAATGTTTG AGAGAGAAAC CAATTGACAG ATTATGATCA 
GATCGAGTTG TCACCCTTCT GCTGTCATTG TTGGGGGGGA GAGAAAGACG 
TATCGAGAGT GTGCGCGGTA GGCATAGAGG CACAACTAGC GAGTGTGTGT 
GTGTGTGTGT GTGTGTTTGA AAGAGGAGTA GATAGATTAT TATCAAGATC 
GATGTGCCAC CTACTAGCTC CTTCGGTGCC GGGTAGTGTG TGTGTGTATG 
TTTGAGAGAG AAGCCAGTCG ATAGATTAGT ATCAAGATCG AGGTGTCACC 
CTACTCCTTC GGTGTTGGGA AGTGTGGGTG TGTGGGGAGG GGGGCAGTGA 
CACTCACATA TCT
Go back to index
wmc(5c8) Plate reference : 5c8
WMC locus number : WMC Length of sequence : 214
Last updated : Tue, Feb 11, 1997 at 1:08 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence : 214  Consensus sequence is :

..

TAATAAGTCA CATTAGAAAG GTGCAGCGGG ACCAAGGTGC AAGCAATTTT 
TTTTTTCGAA ACGAGGTGCA AGAAGATNGG AGGAGAAGGA GAAACTTGAC 
CAGTCCTTCG TCCAATCAAA GCCCCGATTT CTGTCTCTAA ACGCAACAAT 
TAATCACAAC AACTTAGAGA TGCTGTTTTA GCTACCATGC AGGGACCACG 
TATTAACTCG CATG
Go back to index
wmc140(5d8) Plate reference : 5d8
WMC locus number : WMC140 Length of sequence : 177
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)12 78 to 101.
PCR Comments : 61C anneal, smear; 51C anneal, product;
Forward primer details :
cccTcTccTcgggTgTTgcTTg at 52 length 22 bases, Tm 71
Reverse primer details :
cccAggAgcccTcATgcATAcg at 130 length 22 bases, Tm 72
Expected size of amplified fragment (Chinese Spring=) 100 bases.


Length of sequence : 177  Consensus sequence is :

..

CCTAGANCTG TATGGAGAAA AGGAGGAAGG CTGGCCAGGG CTTAGGCAGC 
TCCCTCTCCT CGGGTGTTGC TTGCTAGGTG TGTGTGTGTG TGTGTGTGTG 
TGATCGCCTG TGCGAATGAA GGAGTTGCCC GTATGCATGA GGGCTCCTGG 
GGGGTTTTAT AGGTCAACCC CCCAGGG
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wmc(5e8) Plate reference : 5e8
WMC locus number : WMC Length of sequence : 119
Last updated : Tue, Feb 11, 1997 at 7:27 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)28 16 to 71. No room for primers.
PCR Comments : 


Length of sequence : 119  Consensus sequence is :

..

GTTAATTCTC ACCATGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TACGTGAGAG AGTTGAGAGG GAGAGAGAAG 
AGAGAGTTGT GTCAGGCAA
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wmc(5f8) Plate reference : 5f8
WMC locus number : WMC Length of sequence : 172
Last updated : Tue, Feb 11, 1997 at 7:28 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (TTG)19 2 to 58, (TTG)31 80 to 172. No room for primers. Matches 9A3, 9C5, 9C6.
PCR Comments : 


Length of sequence : 172  Consensus sequence is :

..

GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT 
TGTTGTTGCT GTTGTTGCTG TTGCTGCTGT TGTTGTTGTT GTTGTTGTTG 
TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT 
GTTGTTGTTG TTGTTGTTGT TG
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wmc(5g8) Plate reference : 5g8
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 11, 1997 at 11:27 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No sequence 11.2.97
PCR Comments :
No sequence available for this clone
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