Wheat microsatellite WMC 25/06/01

wmc(5h8) Plate reference : 5h8
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 11, 1997 at 11:27 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No sequence 11.2.97
PCR Comments :
No sequence available for this clone
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wmc141(5a9) Plate reference : 5a9
WMC locus number : WMC141 Length of sequence : 248
Last updated : Lun 9 oct 2000 at 14:43
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end (CA)15 66 to 95. Complex. Bases 174 to 195 show homology to TW2e04.1 bases 151 to 172 (22 in longest run, maxScore = 23).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TgcAAggggTTgAgTTcTTAgg at 40 length 22 bases, Tm 64
Reverse primer details :
TTgATAgAgcAAgAcgAAgggc at 117 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 99 bases.



Length of sequence : 248  Consensus sequence is :

..

CCCTGAGAGG TAGGCATATA TAAACCCCCA GGGCACCCAT GCAAGGGGTT 
GAGTTCTTAG GGTTTCACAC ACACACACAC ACACACACAC ACACAGAGAG 
GAGGATAGAT AGACTTGCCC TTCGTCTTGC TCTATCAAAC ATCTCAAGGA 
TCCTCTTGTA ACCACTTACT GATTTAGTGA TCATGCGGAG ACCCCACAGA 
GCAGGACTAA GGGTGTTATC TCCTAGGAGA GCCCCAAACC TGGGTAAG

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wmc(5b9) Plate reference : 5b9
WMC locus number : WMC Length of sequence : 263
Last updated : Mer 23 DŽc 1998 at 11:26
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)10 82 to 101, (GT)10 170 to 189. Bases 78-189 (ie 3' side of microsatellite) match part of 3A7 complement. Close relative of 7h1. Sequence contains repeated structure (111-165 matched to 204-258) - - too difficult to make primers. Bases 170 to 206 show homology to 22e11 bases 44 to 80 (37 in longest run, maxScore = 74). Bases 85 to 168 show homology to 2e6 bases 446 to 529 (complement) (84 in longest run, maxScore = 112). True duplicate with 2e6, 5b9 has priority.
PCR Comments :



Length of sequence : 263  Consensus sequence is :

..

GTCATTGTAG GGGTGGGGGA GGGGAGGGAG AGAAAGATGT ATCAAGAGTG 
TGCGCGGTAT GCACAGAGGC ACAACTAGCG AGTGTGTGTG TGTGTGTGTG 
TTTGAAAGAG GAGTAGATAG ATTACTATCA AGATCGAGGT GCCGCCCTAC 
TCCTTCGGTG TCGGGTAGCG TGTGTGTGTG TGTGTGTGTT TGAGAGAGAA 
GCCAGTCGAT AGATTAGTAT CAGGATCGAG GTGTCACCCT ACTCCTTCGG 
TGTTGGGAAG TGT

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wmc142(5c9) Plate reference : 5c9
WMC locus number : WMC142 Length of sequence : 466
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Complex small microsatellite motif (CG)2(ACTC)2(AC)2(GC)6 at 296-328. Microsatellite is buried in domains of larger repeats (4 repeats 5', 2 repeats 3'), take care if designing new primers.
PCR Comments : tested 61C anneal, no product; 51C anneal, product;
Forward primer details :
AAccgcAAcTcTTgTgAccgTg at 231 length 22 bases, Tm 68
Reverse primer details :
gcTAgAccTgccTgTcggTATg at 352 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 143 bases.



Length of sequence : 466  Consensus sequence is :

..

GCCGCCGTGT AACGCACCTG CAGCTGCGCT TATTGCCTAC CTACCGCCGC 
CGCGCAAAGC ACCTGCAGCT GCGCTTATTG CCTACCTACC GCCGCCGCGC 
AACGCACCTG CAGCTGCGCT CATTGCCTAC CTACCGCCGC CGTGTAACGC 
ACCTGCAGCT GTCATTATTG CCTCGCCACC ATCTGCGATG CGCACGTCGC 
CAGACGCCGT CTTCGACATC GCCTAGGCCA AACCGCAACT CTTGTGACCG 
TGTTGGCCAT GCGACTAAGT GTGGCCGCTA GGACTCTCTT GCACCCGCGC 
ACTCACTCAC TCACACGCGC GCGCGCGCTG CAGCTGGCCG ACCACCATTA 
CCATACCGAC AGGCAGGTCT AGCCCAGATA CATAACCTAA CCTCCGTCCA 
AAATGACACA GCATTGGCGC GTCGGGACCG CGCCTCCTTG CGAGAGGGAA 
GTCCCGCTAC AACTTT

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wmc(5d9) Plate reference : 5d9
WMC locus number : WMC Length of sequence : 449
Last updated : Tue, Feb 11, 1997 at 7:32 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)8 37 to 52, (GT)16 5 to 36. No room for primers.
PCR Comments :



Length of sequence : 449  Consensus sequence is :

..

GGAAGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTCACA CACACACACA 
CACNGCAGCA CGGCAACAAC AACAACGAAT TGGTTACCAA CGATTANTNG 
AGGGGGAAAG CGAGNCNGTT GTGGGATTCA GGGAACACAA CAANAGTGGA 
TTNGGCATGG TTACNAACNG CGAATGGCGG GANAATTGTA ATTAGACTGG 
TANGNTGTGG TGTTTANGNG ACGGTGGTGA ACCACCACCA TCCAACACAG 
ACTCGAAGTT GTGTGTTGTG TTTTGCACGC ATGCAGAATA TAATTTNTCT 
CATCCTAGTA GTGTTAACGA CATACTTTTT GGTATTTGGA AAACAAATTT 
TGAGTTTGAA TGGAACACGA CTTCTCTNTG TTCCTCACTA TTTATTTCCC 
AAATGACTAG AACAATGCCC GTGCGTTGCN ACGTGATATA AATATTNTA

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wmc(5e9) Plate reference : 5e9
WMC locus number : WMC Length of sequence : 85
Last updated : Thu, Aug 21, 1997 at 11:31 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)22 38 to 81. No room for primers. Matches 5c3.
PCR Comments :



Length of sequence : 85  Consensus sequence is :

..

ATGCTCTCTC TCTCCGATTG TAGCATACCG GCAGCATCAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACTAA

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wmc(5f9) Plate reference : 5f9
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 11, 1997 at 11:27 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : NO INSERT
PCR Comments :
No sequence available for this clone
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wmc143(5g9) Plate reference : 5g9
WMC locus number : WMC143 Length of sequence : 426
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)9 206 to 223, (CA)19 234 to 271. (2 loci). Bases 108-223 (ie. 5' side of microsatellite) match 11c5. Bases 240 to 415 show homology to 2f4 bases 103 to 278 (176 in longest run, maxScore = 124).
PCR Comments : 51C anneal, product;
Forward primer details :
cTTgAcccAAgTAgTTcTTTcc at 177 length 22 bases, Tm 59
Reverse primer details :
gTgggTAAAgTgTgcAAccTcT at 367 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 212 bases.



Length of sequence : 426  Consensus sequence is :

..

ATATAGCTCT AAGTAGGTAA TTTTTACAAA AAAGCTAGTT TGAGTGCAAT 
GACATGTCCT AACAACTTGT AAATCATGCA TCATAACAAT GTAAGTAACG 
TGGGGACGGG TTACAGATAT CAACATAAAC AGAGTTTGGA TCAACTCCAG 
CTTAATCAAC AAGTCAAGTC TTTTGACTTG ACCCAAGTAG TTCTTTCCAC 
TCATCCACAC ACACACACAC ACAACACACA CAACACACAC ACACACACAC 
ACACACACAC ACACACACAC ACTAGGTTTT GTAAACATTT GGATGTAGCA 
CAAGAGCGGT TATCCAAATG TCCTTGACCG TGGACACGGA TATTTTTGAT 
TAGTTTTACA CTCTACAGAG GTTGCACACT TTACCCACAA GATCCGGGAA 
GCTTTCATGT CATCCNNCGA CCCGCG

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wmc(5h9) Plate reference : 5h9
WMC locus number : WMC Length of sequence : 261
Last updated : Mer 14 juin 2000 at 10:34
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)8 170 to 185. No room for primers. Bases 26 to 74 show homology to 10e1 bases 179 to 227 (49 in longest run, maxScore = 56), true duplicate, 5h9 has priority. Bases 11 to 29 show homology to TW1h05.2 bases 1 to 19 (complement) (19 in longest run, maxScore = 22).
PCR Comments :



Length of sequence : 261  Consensus sequence is :

..

AGTTACCTCA TCAAGTTCTA CTTTCCTCCC ACTCACTTCT TTCGAGAGAA 
ACTCCTTCTC TAGAAAGGAT CCATTCTTAG CAACAAAGAT TTTTGCCTTC 
GGATCTGTGA TAGAAGGTGT GTGAGTGAGT GATGTTGGTG TGTGTGGGTG 
GAGGTGGAGT GCTGTTGATG TGTGTGTGTG TGTGTGAGTG AGTGATGTTG 
GTGTGTGGGG GGGGTGCCGT TGAAGTGTGT GTGATGTGTT TGTGTGTGCG 
TGTGAGTGAG T

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wmc(5a10) Plate reference : 5a10
WMC locus number : WMC Length of sequence : 273
Last updated : Tue, Feb 11, 1997 at 7:35 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)8 234 to 249. No room for primers.
PCR Comments :



Length of sequence : 273  Consensus sequence is :

..

TTCGTTGGTT TCAACATAGC CCTTCCCGTA GCGATCCACT GCTTCCATCC 
TGATTATGCC TCCTGCGTGA AATTTTTGTA TGCAAGTTCA GTGTGCTATG 
ATGTTCTATA TGATTGTGTC AACTATATAA GTTTTTGCTG AGCATCAGCA 
ATGCATATGG CTGAAAGATG TATTTACGAG CATCGACCGA TGGGTCTCTC 
TCTATCTCTC TCTATCTCTA TCTCTCTCTC TCTCACACAC ACACACACAC 
GCACACACAC ACGCGCACAC GCA

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wmc(5b10) Plate reference : 5b10
WMC locus number : WMC Length of sequence : 246
Last updated : Tue, Feb 11, 1997 at 1:15 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :



Length of sequence : 246  Consensus sequence is :

..

TTGCCATNTG CACAGCCGCC GTCCCCACGC CAATCCTAGT CTAACGCTTC 
CCCCCGCCGC CGCAGTCCCC AATGCTGCTC GCCGTCGATC ATTTCCCACG 
CCGCGTCGCC GTAGACCATT CCCATTCTCA GCTCTCCCCA GGGCCGCTCG 
CCGTCGACCA GTCCACCGTG CGGCCGTTCC CCCAAGTCGC GTCACCCTCC 
GTCAATCTCC CTCGCCCATT CAACGCCGCT CGCCCATCGA CCCCTG

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wmc(5c10) Plate reference : 5c10
WMC locus number : WMC Length of sequence : 347
Last updated : Tue, Feb 11, 1997 at 1:16 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 61C anneal, product;



Length of sequence : 347  Consensus sequence is :

..

GAGGTGAGGA GATACACGAA GATAGCTAGT GTGTGATTGT TTGCAACGGA 
GAGAGACACC CCTATAGCAC ATGTCCAATA GAGGCGTGGC CAACAAGGAA 
CAAAGGTCGA GAGGGACGCC TGGATGGCAT GGACGCATGA GGAGGGAGAG 
AGGGTGTGTT TGTGATAGAG AGATCCCGTC AAGATCATGA GGGGACTATA 
TGGGTGGGAG ATCGAGGGAG TGCGTGCGCA ATAGAAAGAT GCTCCGAGAG 
AAACCGAGAT AGACCATGCA CATGTTTGTG ATGGAGACTA AGATTAGCTA 
GTCATATACA TATAGGGGGA CTGCGTGTGC CTACAATTGA GTGAGAG

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wmc(5d10) Plate reference : 5d10
WMC locus number : WMC Length of sequence : 230
Last updated : Tue, Feb 11, 1997 at 7:37 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)32 166 to 229. No room for primers.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;



Length of sequence : 230  Consensus sequence is :

..

TCGCAACATT AACGTAACAT TTGCAGCAAA CACCCAGAAT CAACGCGACG 
TGCTGCACTT CTATTTGCAT CAACAAAAAA TTGAGGAACC AGCAGAAGCT 
TTGCCTTTTC ATTAAGAAGA GAATTGACCA GTTAATAAGA AAAAACTGAA 
AATCACTAAA TCTACCACAC ACACACACAC ACACACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC

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wmc(5e10) Plate reference : 5e10
WMC locus number : WMC Length of sequence :
Last updated : Tue, Feb 25, 1997 at 22:20
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Clone contains multiple inserts (2) joined 164-181 by linker sequence No substantial microsatellite.
PCR Comments :



Length of sequence :   Consensus sequence is :

..

GTGGCACTGG GAGTTCAGGC AGCCGCTGCC GGACCCCGAC ATTACGCTGC 
CGCACCACTG GCACCTCAAT CCGCAGCGGA CCACTATGCT AGCGGTTCCA 
CGGACACAGC GGGTGCACGA CGAGGAGGTG CGCATGCGCC GGAGCAGTAG 
CGCATGCCCG AGTTAGTCCA CGCGTGGACT AACTAAACGT TCCCGATACG 
GCGATACGCA CGATACAGCT AGGATACGGG TATCGGCGAA GTATCCTATT 
TTCCGATTTA AAAAGAAAAG AAAAAACGTT GATACGTCCT GTGCATCGTC 
TACGATACGG GAGCGACACG GCAGCCCACT GGAAGGCCCA TGGCTAACCC 
TAGCATCTGG GCTCCCTCTC TTTTGTTTTG TTCGCACGAT AGGAGCTTGC 
ACGAGGGAGC CGCCGCCGCT GGACAAGCCT CCCCGCCGGC CGCAGCCGCA 
GATTGGAGTC ACTCGCCGCC TGGAGTGGCT CACCGGCGCC GCCTGGAATT 
GCCTCGCTGC CGCCTCAACT CGCCCCCACC GGCTGGAATC GCCTCTCCGC 
TTGAATCTTC TGCT

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wmc(5f10) Plate reference : 5f10
WMC locus number : WMC Length of sequence : 283
Last updated : Tue, Dec 9, 1997 at 11:00 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite. Bases 122-283 match part of 13C2 (complement).
PCR Comments :



Length of sequence : 283  Consensus sequence is :

..

ATGGGATATT CACGTGATCT ACTTGATGTA TGTTTTGGTG ATCAACTTGC 
GGGTTTTGTG ACCTTATGAA CTTATGCATA GGGGCTGGCA CACTTTTTCG 
TCTTGACTCT CCGGTAGAAA CTTTGGGGCA CTCTTTGAAG TTCTTTGTGT 
TGGTTTGAAT AGATGATTCC GAGATTGTGT GATGTCATAT CATATAATCA 
AACCCGCGGA TACTTGTGGT GACATTGGAG TATCTAGGTG ACATTAGGGT 
TTTGGTTGAT GTGTGTCTTA AGGTGTTATT TTA

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wmc144(5g10) Plate reference : 5g10
WMC locus number : WMC144 Length of sequence : 247
Last updated : Thu, Aug 21, 1997 at 11:20 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)14 69 to 96. Bases 70-247 (complement) ie 3' side of microsatellite match 1b4, 5f2.
PCR Comments : 61C anneal, product;
Forward primer details :
ggAcAccAATccAAcATgAAcA at 7 length 22 bases, Tm 65
Reverse primer details :
AAggATAgTTgggTggTgcTgA at 128 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 143 bases.



Length of sequence : 247  Consensus sequence is :

..

AGAGTTGGAC ACCAATCCAA CATGAACATT GTGTCAAGTC ATCTGACTTA 
ACCCAAATAG TTCATCAACA CACACACACA CACACACACA CACACACGCG 
GTTTGTAAAA ATCTGGACGT AGTTTAATCA GCACCACCCA ACTATCCTTG 
ACCGTGGACA CNGCTATTCG AATAGTTTTA CACTCTGCAA AGGTTGCACA 
CTTTACCCAC AAGATCCGGG GAACTCTCGT GTCATCCATG ACCCGCG

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wmc(5h10) Plate reference : 5h10
WMC locus number : WMC Length of sequence : 250
Last updated : Tue, Feb 11, 1997 at 1:37 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite. Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 9H3 etc
PCR Comments :



Length of sequence : 250  Consensus sequence is :

..

AGATGATAAG TTTGCTGACT ACTAAAGTGA AGTGGAATTT GTCCATGTTA 
TGCAAGTAAA TAAAGGTGAT TGTTTATCAT ACGGGTAAGG TTGGATGAAG 
GGTGGTAGGA ACAAATGCTC CGCCTAGAGC ATGTGTGTGT GGGATGGAGT 
CTTAGTGTGT GTGTGTGTGA GGGAGAGAGA GAGAGGAGAG AGAGAGATGG 
AGTCTTAGTT NGTGTGTGTG TGTGAGAGAG AGGGAGAGAG ATGGATCCTA

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wmc(5a11) Plate reference : 5a11
WMC locus number : WMC Length of sequence : 226
Last updated : Tue, Feb 11, 1997 at 11:27 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 226  Consensus sequence is :

..

GACAACACAC ACACACGCAC GCAAAGGTCG TCCTGGAACT GATTCAGGGC 
GGACATAGTG CTCCTGGCTA GCCACCGTGC ATGGACACAA ATTTGGACAC 
GGGGATGCTT CCCAGAAGTT GCTTGGGCGC ACCATGCATA TAGAGGGCAC 
ACGCACGCAC CCGCCCGGGC CCGCGCTGAC ATACTCTTCA TCTCCATCCA 
ACCGCGTGTG GCTACCTCCT CCTTCG

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wmc(5b11) Plate reference : 5b11
WMC locus number : WMC Length of sequence : 388
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite. Bases 72 to 84 show homology to 12d1 bases 17 to 29 (13 in longest run, maxScore = 48). Bases 50 to 66 show homology to 21h1 bases 26 to 42 (complement) (17 in longest run, maxScore = 22). Bases 327 to 343 show homology to 26h3 bases 66 to 82 (complement) (17 in longest run, maxScore = 20).
PCR Comments :



Length of sequence : 388  Consensus sequence is :

..

GCATGGTCAT CTTCTCAAGC TCAGAGGTGA TGTTCTCATT CGAGCCAGAG 
TCAAGATACC AGTTGGAGTC AACTCCGTAT GAGGCATCTA CCGCGGCTGC 
AACACGCCTC CTTCGAGAAT TGTCCTCTGC ATAGCGCCAC TTGCACTCTT 
TCGCAATATG GTTAGTTTTC TTACAGATTT GGCATTTGTT TTCATACTCC 
TCATACCCCG CGAAGCGACC GCCTTGATTG TTGTTGTAGT AGTGGCGAGG 
GGGGCCGTTG TTGTTGTAGT AGCCCCCGCC ACCGCCGCCA CGATGGCCAC 
CGCCTTGACC ACCGCTGTTG CCGTAGTTGT TGTAGCCGCC GCCACCGCCA 
TGGTTGTAGG CGCCGCCACC CGCGCCAGGA TAGCCGCC

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wmc(5c11) Plate reference : 5c11
WMC locus number : WMC Length of sequence : 64
Last updated : Tue, Feb 11, 1997 at 7:41 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Core sequence similar to 1d1. (GT)9 6 to 23. No room for primers.
PCR Comments :



Length of sequence : 64  Consensus sequence is :

..

GTGTAGTGTG TGTGTGTGTG TGTTTGTGTG TGTCTGCGTG TATGTGCGTG 
TGTTCTGTGT TTGT

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wmc145(5d11) Plate reference : 5d11
WMC locus number : WMC145 Length of sequence : 281
Last updated : Thu, Aug 21, 1997 at 3:38 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : complex small microsatellite GTGGCCG (CGG)3TGG(CGG)2C at 77 - 102. Matches 5C6.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
ggcggTgggTTcAAgTcgTcTg at 40 length 22 bases, Tm 72
Reverse primer details :
ggAcgAgTcgcTgTccTccTgg at 225 length 22 bases, Tm 72
Expected size of amplified fragment (Chinese Spring=) 207 bases.



Length of sequence : 281  Consensus sequence is :

..

TTCGACCAGA GAGTCGCGCT CTTCCAAGGA AACAACTCCG GCGGTGGGTT 
CAAGTCGTCT GCTAACACCG CTTCACGTGG CCGCGGCGGC GGTGGCGGCG 
GCTCCTCACG CTACACCAAC CCTAAGGGAC GCCGCAACAA CTCCAGCAAG 
TCCCATCCAG ATGCTGTCCG CTGCCAGATT TGTGGCAAGC TTGGACACTC 
GGTGAGGGAC TGCTGGTATC GCTTCCAGGA GGACAGCGAC TCGTCCGGTG 
ATGAGAAGGT CGCTGGAGGA GCGGATGGCT C

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wmc(5e11) Plate reference : 5e11
WMC locus number : WMC Length of sequence : 493
Last updated : Wed, Aug 20, 1997 at 1:40 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)9 12 to 29. No room for primers. Matches 3c4. Bases 280 to 339 show homology to 12g11 bases 290 to 349 (complement) (60 in longest run, maxScore = 160).
PCR Comments :



Length of sequence : 493  Consensus sequence is :

..

CGCGCGCGCA TGTGTGTGTG TGTGTGTGTT TGTGTGTGTG TGTATGTGTG 
TGTGTGTGAG AGAGAGAGAG AGGGAGGGAG AGAGAGAGAG CCAANCTCAT 
ATCAACAGGT GCTCTGCTGG AATCCCATTG TATGTATTCA TATGGTTCTG 
GAACTCGAGT TAACCTTAGG CATATGTGTG AGAGACATAA TTATACTTTG 
AGACATTAGT GGTTGTGGGT GGGCGGAATT AAAGGGGTGG GCTTGTTCGA 
CAGAGAGAGC GTGTGTGTTT CTGTGTGATA GACTTGTAGG ACGGGGTAGA 
AAGACGAATT CTAACCTTGA CGGAGGGAGA GAAATACACA AAGTGCGCGT 
GTGTGATTCC GATGCCCACA GGGAAACGAG ATATGTATGC GTGTGAGAGA 
GATTGCACAA TTCATTCGTG TTTCACTATC TAGCGATCAT GGACGTGCAT 
CGCTTGAACA TAAATCAATA TCTACTTCGT ATCGTGGCCA AAG

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wmc(5f11) Plate reference : 5f11
WMC locus number : WMC Length of sequence : 340
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite. Related to 1C4. Bases 131 to 153 show homology to 22c1 bases 160 to 182 (23 in longest run, maxScore = 73).
PCR Comments :



Length of sequence : 340  Consensus sequence is :

..

ACACACACAC ACCCACACAC ACACACACTT ATGAGGACAT GGTTGCATAA 
ACAAATTAGA CATCCACTTA CNTAGGGTGG CTACTACAAC GATGGCATTT 
ACACACACCA AAGTAAACTA TTATATGCAT AATTACATAG GTGGCTACTA 
CACACAAGAC ATTTCCACAC ACCAAAGTAA ACTATATATT ACATGCATGA 
TTAACTAGGA TAAACGATCA TCTGATCATC ATCTACTTCT TGTGATGCTT 
GCTCAGGTTG TGGCTCGATC CGGCCTCGTT GATCTCCGTA ACAAGGCACT 
TTCTCATGTC AACGATCCGC ATGTGCATCT CGTAGCATGT

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wmc(5g11) Plate reference : 5g11
WMC locus number : WMC Length of sequence : 186
Last updated : Lun 9 oct 2000 at 14:43
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GA)17 2 to 35. No room for primers. Matches 9B3, part of 11g1. Bases 140 to 150 show homology to 10g10 bases 146 to 156 (11 in longest run, maxScore = 21). Bases 55 to 63 show homology to TW3b01 bases 192 to 200 (9 in longest run, maxScore = 21). Bases 138 to 146 show homology to TW4b09.1 bases 160 to 168 (9 in longest run, maxScore = 22). Bases 120 to 134 show homology to TW5h05.2 bases 118 to 132 (15 in longest run, maxScore = 38).
PCR Comments :



Length of sequence : 186  Consensus sequence is :

..

AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAAGGCC NTCTAGCTAC 
TAGCTATGGA CCCATAGGTC TGTGGTAAAC TACTCACGGT TCATCGGAGA 
GGCAATGGTG TTGATGTATA AGCCCTCCAT GATCGAATCC CCCTCCAGCA 
CGATGCCGGA AAAGGACCCA AGATGGGATC TCACGG

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wmc(5h11) Plate reference : 5h11
WMC locus number : WMC Length of sequence : 387
Last updated : Tue, Feb 11, 1997 at 1:41 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite. Bases 6 to 16 show homology to 12c12.2 bases 99 to 109 (11 in longest run, maxScore = 20).
PCR Comments :



Length of sequence : 387  Consensus sequence is :

..

CGCAGCGTCG CCGCCGACAA GCTTCACCGC AGCCACCGCC ATCCATTCGT 
TGCCCCGTCA TGCCAGGATG CTCCGCCGTC GTCGACTACA TCAAGTAGGC 
CGAGCCCCGA GCGCTCGCTC GCCCTGGAGA CGCTGCACCG AGCTCGTCCT 
CAACTCCGGC CACCGGAGAT CCCTTTCGCC GCCCCGCTCG CTCCAGCCCT 
TCCCCGAGCT TGCCGTTGGC TCCCCTGCAA AAGCTGTGAG CTCCACCACC 
GTTTACCCCT CTCCCCGCGC TCCTCCGCGT CCTCTAACCA CCTCTGCCAT 
CAAAGCCGAA GCTCGCCGCC GCCACCGCAC GTCGCCGTCG TGGCCACAGC 
CACGTTGGGA TGCCACCGAG CACGCCTTCG TCTTCNA

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wmc(5a12) Plate reference : 5a12
WMC locus number : WMC Length of sequence : 341
Last updated : Tue, Feb 11, 1997 at 7:45 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (GT)10 64 to 83, (GT)8 86 to 101. No room for primers.
PCR Comments :



Length of sequence : 341  Consensus sequence is :

..

TATGTGTGTT TGTGTGTGTG AGAGAGAGAG AGATGGAGTC TTANTTTGTG 
TGTGGGGGGG TGCGTGTGTG TGTGTGTGTG TGTTTGTGTG TGTGTGTGTG 
TCGTGGGGTC CTCTGTGGCG GATGTAATTT AAGTGTGCAT GGCATAGAGA 
GGTGATGTGT ATGGCAGAGG CCACAAATGA TGAGGCGTGA GAGAGAAAAT 
GCCCGTAGGA GGGAAGATAA GGTGCGTGCG CGTGAGAGGA GTCGACATCG 
AGAGACGTGT TTGTTCAAGA GACACCGAGG AAGACTACTA TATATCGATG 
GTACATGTAG GCGGGAATTA AACAAAGGGG TGGTNTTATT G

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wmc146(5b12) Plate reference : 5b12
WMC locus number : WMC146 Length of sequence : 353
Last updated : Thu, Aug 21, 1997 at 11:11 am
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)31 137 to 198. Bases 243-322 ie. 3' side of microsatellite match 5g10, bases 148-331(ie 3' side of microsatellite) match part of 5F2.
PCR Comments : 61C anneal, product;
Forward primer details :
ccAAgTggTTcTTTccATcATc at 115 length 22 bases, Tm 62
Reverse primer details :
gTgggTAAAgTgTgcAAccTcT at 292 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 199 bases.



Length of sequence : 353  Consensus sequence is :

..

GTTCAACAAT TCGTAAGCAT GCATCATAGA ATTAGTACCA TGAGATAGTT 
ACAGATATCA CATAACAGAG TTGGATCACT CCAGCTTAAT CAACATGTCA 
GTCTTTGACT TGATCCAAGT GGTTCTTTCC ATCATCCACA CACACACACA 
CACACACACA CACACACACA CACACACACA CACACACACA CACACACATC 
AGGTTTCGCA AACATTTGGA CGTAGCCCAA GAGCAGTTAC CTAACTGTCC 
TTGACCGTGG ACACGGCTAT TCGAATAGTT TGATACTCTG CAGAGGTTGC 
ACACTTTACC CACAAGATCC GGGAAGCTTT CCCTGTCATT CACGATACCC 
GCA

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wmc(5c12) Plate reference : 5c12
WMC locus number : WMC Length of sequence : ~1000
Last updated : Lun 30 oct 2000 at 16:59
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : INSERT ~ 1000 BPS, SEQUENCING GOOD QUALITY IN ONE DIRECTION ONLY, THEREFORE NO OVERLAP.(CAA)13 173 to 211. No room for primers. Relative 7h7. Bases 146 to 177 show homology to 17f12 bases 12 to 43 (complement) (32 in longest run, maxScore = 40). Bases 284 to 297 show homology to 19d4 bases 93 to 106 (complement) (14 in longest run, maxScore = 19). Bases 173 to 192 show homology to 10f11 bases 130 to 149 (20 in longest run, maxScore = 40), duplicate with 10f11, 5c12 has precedence. Bases 134 to 143 show homology to 22c11 bases 190 to 199 (10 in longest run, maxScore = 21). Bases 173 to 204 show homology to 24a5 bases 23 to 54 (complement) (32 in longest run, maxScore = 35). Bases 185 to 213 show homology to 20b4 bases 187 to 215 (29 in longest run, maxScore = 55). Bases 134 to 153 show homology to 25e12 bases 336 to 355 (complement) (20 in longest run, maxScore = 29). Bases 173 to 221 show homology to 20c7 bases 84 to 132 (complement) (49 in longest run, maxScore = 43). Bases 173 to 213 show homology to 26d11 bases 70 to 110 (41 in longest run, maxScore = 31). Bases 174 to 213 show homology to TW3c05.2 bases 34 to 73 (40 in longest run, maxScore = 38). Bases 305 to 334 show homology to TW2e12.2 bases 221 to 250 (complement) (30 in longest run, maxScore = 45). Bases 86 to 105 show homology to TW5d02.1 bases 4 to 23 (20 in longest run, maxScore = 31). Bases 173 to 213 show homology to TW5e11.3 bases 165 to 205 (complement) (41 in longest run, maxScore = 57). Bases 59 to 84 show homology to TW7d07.1 bases 198 to 223 (complement) (26 in longest run, maxScore = 58).
PCR Comments :



Length of sequence : ~1000  Consensus sequence is :

..

ACAGCANCAG CAGCAGCANC AGCAGCAGCA ANAGTANCAG CAACGACAGC 
AACAGCAACA GCAGCAGCAA CAGCAACAAC AGCAGCAGCA GCAACAGCAA 
CAGCAACAGC AACAGCAATA GCAACAGCAA CAGCAACAGC AGCAGCAGCA 
ACAGCAACAG CAACAGCAAC AGCAACAACA ACAACAACAA CAACAACAAC 
AACAACAACA ACAGCAACAG CCACAGCAAC AGCAACAGCA GCAGCAATAG 
TAACAGCAAC AACAGCAACA GCAACAGCGG CAGCAACAGC AACAACAGCA 
GCAGCAGCAA CAGCAACAGC AACAGCAACA GCAATAGCAA CAGCAACAGC 
AACAGCAGCA GCAGC

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wmc(5d12) Plate reference : 5d12
WMC locus number : WMC Length of sequence : 282
Last updated : Lun 5 juil 1999 at 17:23
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 9H3 . (CA)8 99 to 114, (CT)11 75 to 96. Bases 159 to 225 show homology to 21c4 bases 63 to 129 (complement) (67 in longest run, maxScore = 116), duplicate, 5d12 has priority. Bases 203 to 282 show homology to 26d10 bases 169 to 248 (80 in longest run, maxScore = 97).
PCR Comments :



Length of sequence : 282  Consensus sequence is :

..

GGTCTTTGCT TGTTAGTTTA CCATCTCTCT CTCTCTCACA CACACACACA 
AACTAAGACT CCTCTCTCTC TCTCCTCTCT CTCTCTCTCT CTCTCTCGCA 
CACACACACA CACACTCCAC ACACACACAC TAAGACTCCA TCTCACACAC 
ACATGCTATA GGCAGAGCAT TTGTTCCTAC CACCCTTCAT CCAACCTTAC 
CCGTATGATA AACAATCACC TTTATTTACT TGCATAACAT GGACAGATTC 
CACTTTACTT TAGTAGTCAG CAAACTTATC AT

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wmc(5e12) Plate reference : 5e12
WMC locus number : WMC Length of sequence : ~800
Last updated : Jeu 27 Aož 1998 at 12:05
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : INSERT ~800BPS LONG,NO OVERLAP.(TTG)6 45 to 62, (TTG)6 99 to 116 No room for primers. Bases 683 to 702 show homology to 17a9 bases 150 to 169 (20 in longest run, maxScore = 25). Bases 669 to 691 show homology to 12a10 bases 234 to 256 (complement) (23 in longest run, maxScore = 45). Bases 586 to 648 show homology to 10a9.2 bases 85 to 147 (63 in longest run, maxScore = 53).
PCR Comments :



Length of sequence : ~800  Consensus sequence is :

..

CTGCTGCTGT TGGTGCTGCT GTTGTTTGTG TGCTGCTGTT GCTGTTGTTG 
TTGTTGTTGT TGTANTTGTT GTTGTCGTCN TTGTCGTCGT TGTTGTCGTT 
GTTGTTGTTG TTGTTGCNGT TGTTGTTGCT GTTGTTGTTG CTGTTGCTGT 
TGTTGTTGCT GTTGCTGTTG CTGTTGTNGT TGTTGCTGCT GCTGTTATTC 
TTGTTGTTGT TGTTGCTGCT ATTGCTGTTG CTGTTGGTGT TGCTGTTGCT 
ATTGCTGTTG TTGTTGCTGT TGTCGTTGTC GCTCCTCTTG TTATCCACCC 
TGGACTACTT GAGTTCGGTG CAACCNCATG GGCATCTTGC CAGTAGGAAA 
GATTGTGTGA CACA
(break)
ACTGTCATT CCTTCTGCTC TCGTTGGTTC 
GACACTCTTA CTTATCGAAA GGACTACGAT TGATCCCTAT GTGCTGCCTG 
CGTGTGTGCT GCTGCTGCGT GTGTGTGTAT GTGTGTGTGC TGCGTGCGTG 
TGTGTTGCTG CGCGTGTGTG CGCGTGCATG CGTGTTGCTG CGTGTGTGTG 
TGTGTTGTTG CGTGTGTGTG CGCGTGCGTG TGTGTTGGTG CATGTGTATG 
TGTTGCTGCG TGTGTGCTCC TGCCCTCGCA TTGATGCTGC GTGTGTGCGC 
TATTGTCCCC CACACACATA CCACGTGAGG GGCAAAAGAG TCTTTTCAGG 
TGTCATTTAA GCTCAAAATG GACGGAAATG TNTAGT

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wmc(5f12) Plate reference : 5f12
WMC locus number : WMC Length of sequence : 347
Last updated : Tue, Feb 11, 1997 at 1:47 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :



Length of sequence : 347  Consensus sequence is :

..

GCGAAGATAT AGCTGGACAT CTTCACTCAG CCTAATCTCA ACCTCAGGCA 
GACTCGATGG GTCGAAATGA TTCAAGAGTA TAATCCGAGT ATCGAGTATA 
CTCCAGGCAA GGCCAATGTG ATTGCTGACG CATTGAGCAG GAAGGCTTAT 
GGCAACAGTC TGATTCTCAA GCCTTATCAA CCCGAGCTTT GTGAACCTTC 
CCGCAAACTC AATCTGCAAG TTGTTCCTCA AGGTTTCCTC GCCAACCTTC 
AAGTCTCTCC TATCTTGGAA GACCAGATTA GCGAGGCTCA ACTTCTTAAT 
GCTATGGTGA AGAAGGTGAA GATTGGGATT GCCAAGAGCC AACCCAA

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wmc(5g12) Plate reference : 5g12
WMC locus number : WMC Length of sequence : 257
Last updated : Tue, Feb 11, 1997 at 7:50 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : (CA)10 110 to 129. No room for primers
PCR Comments :



Length of sequence : 257  Consensus sequence is :

..

AAGCGACCCC CCTCTCTTCC TCTCTCTCTC CCTCTCTCTC TGTCGCTCTC 
TCTCCCTCCC TCCCTCTCTA TCTATCTATC TCTCTCTCTC TATATCTCTC 
TCTCTCTCTC ACACACACAC ACACACACAC CCCTCTGCCA CACACACTCG 
ATGTCTCTTC CAAATAGTCT GCCCCCCGCC TGCATCCATG CTATCCCGCT 
ACTACACATG CCTTTGTCTC TCTATGTGAC ACACATGCAC ACCATTTCCC 
CTTCCTT

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wmc(5h12) Plate reference : 5h12
WMC locus number : WMC Length of sequence : 325
Last updated : Tue, Feb 11, 1997 at 1:48 pm
Owner : Henry, Lismore Date at Agrogene : 11.2.97 Public Domain : Yes
Comments : Matches 6d10, 5h7. No substantial microsatellite.
PCR Comments :



Length of sequence : 325  Consensus sequence is :

..

TCACGTATGT CACATGTCNN NAGCTTTCTC ANANACCTAA CTTCTTCCTC 
TCTCTTTCTC TCTCTATCTC CCTCCTTCTG TTTCGTTAGG TTTGTCTCCT 
ACTCTCTCGT CCACATACAT ACAATCTTTC CCGCCCTATC TGCTCCATCT 
GCGCAAGCTC TCTCTGCACG TTTCATTCAC ACATTGGGAT ATGTTCAAAA 
TGTTGCTTTT ATAATGGCTG ATGTAGAGTT ATTGTTGGTT TTGTTGCATC 
CTACAAAGTA ATAACTATGA AATGCATTTT GATTGTCATT TGACTGGGAC 
TATGTGAGTT TGAGCATGTT GTGAA

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wmc(11A1) Plate reference : 11A1
WMC locus number : WMC Length of sequence : 282
Last updated : Fri, Feb 21, 1997 at 12:10 pm
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.1.SEQ. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 282  Consensus sequence is :

..

GTATGTAGTC ACTTGTTGAA ATCTCTAGAA AGACAAATAT TTAGGAACAG 
AGGGAGTAGG ACATTTGTCC CGCCGCCGCC GCCACCTTGT CTCATCACCC 
ATCTTCTTCC TCGGGATCCT CCCAGATCCC CTTCTCACCC AACCAGCACT 
CGAGGCCTCC CCTCGATCAA CCTGAGCCAG CCACTGATCC AACCCATCCA 
CCACCTCTCG TGCCTTCCCA GGCTCGACCC CGCCGCCTCA CGATGCTCGC 
CGGAGCAACC AGCGACGACC CTCACCTCCA GG

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wmc147(11b1) Plate reference : 11b1
WMC locus number : WMC147 Length of sequence : 185
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.2.SEQ. Trimmed to Rsa1 at 5', and insert at 3'.(CA)15 99 to 128. (3 loci).
PCR Comments : 61C anneal, product;
Forward primer details :
AgAAcgAAAgAAgcgcgcTgAg at 18 length 22 bases, Tm 69
Reverse primer details :
ATgTgTTTcTTATccTgcgggc at 148 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 152 bases.



Length of sequence : 185  Consensus sequence is :

..

GTACGTATGT TGAAACGAGA ACGAAAGAAG CGCGCTGAGC GCCACCCCAC 
ACCACACAAA CCCATTGCGA GAGACAGAGC AGAGATAACA GCAGGGAACA 
CACACACACA CACACACACA CACACACAAA GCACCAGCCA GCCGCTAGCC 
CGCAGGATAA GAAACACATG GGCCCCGGAA TAGCC

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wmc(11c1) Plate reference : 11c1
WMC locus number : WMC Length of sequence : 209
Last updated : Wed, Aug 20, 1997 at 12:10 pm
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.3.SEQ. Trimmed to insert at 5' end, trimmed off PUC at 3' 20.8.97. No substantial microsatellite. Matches 4C9.
PCR Comments :



Length of sequence : 209  Consensus sequence is :

..

CCCTGCAGGA TTGCCACGCT CCTCCACCAC CACCACGCCG TCGTCCTGCT 
GTTGGACGGA GTCTTCCCCA ACCTCTCCCT CTCTCCTTGC TGGATCAAGG 
CATGGGAGAC GTCACCGGGC TGCATGTGTG TTGAATGACC TGCTAGATGC 
TCTGTCAATG GACATTGATC TCGTCCTGGA CGCTAGCCGG CAGGTTGATG 
GCTATTGAA

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wmc(11d1) Plate reference : 11d1
WMC locus number : WMC Length of sequence : 217
Last updated : Tue, Feb 25, 1997 at 22:25
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.4.SEQ. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 217  Consensus sequence is :

..

GCGGCGCAGG ATCGGAGGGA AAGCTTGGCT GGCTGGCTGC AAAGTTGCAC 
GTCGGATTCC ACTTCCCGGC AGGCGGCAGC GGCAGCGGCG GCGGCAGCTG 
GCTGGTGTAG TGTAGGCGTA GAGAATAAAT ATCTCTGAAT TCATATGGAG 
GATGTAGAAT GGTGGTGGTA GTGGTGGTGG TGGCCGGTGC TGTTGTTGTT 
GCAAGTTGCA AAGCAAG

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wmc(11e1) Plate reference : 11e1
WMC locus number : WMC Length of sequence : 374
Last updated : Fri, Feb 21, 1997 at 12:11 pm
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.5.SEQ. Trimmed to insert at 5' and Rsa1 at 3' end. Identical to 11e2. No substantial microsatellite.
PCR Comments :



Length of sequence : 374  Consensus sequence is :

..

CATGAGATTC TGGTGGTTTG TGCATTATGC TTCTACATGT GGAGCTTTCT 
GGATTCTAAC ATGTGGCAAA ATCTGGTGGA ATGTAAGGTT AGGCAAGTTT 
GATTAACTTA GGTGAGTGTT TGGTTCAAGC CACACCTTAG GCAAGCCACA 
CTAAGGCCCC ACATGACATA CACACACAAA ATGTGGCAAG ATTCCCTTAG 
GCTTGCCAAC TTGTGGCTCT CATTTTGAAG AACTAATAAC TTTGCCTAAG 
CTTAGTTGTG GCAAAGTTAG TCATGAATTA AGTTCAACCA TGGAGTCTTC 
TAGATTATAC ATGTGGCAGA ATCTGGTGGA TGATATCCAA CGGCCAGTAG 
TGCTCGGATT TGCCATCTTT GTAC

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wmc(11f1) Plate reference : 11f1
WMC locus number : WMC Length of sequence : 121
Last updated : Wed, Feb 26, 1997 at 11:25 am
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.6.SEQ. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 121  Consensus sequence is :

..

CCTCGTCCTC TGCCATGGGG CCGATGCCGG TCACGACCTC CTCAAGCGCG 
TCAACCAAGT AGGGAAGAAG CTTGGCGGGG CAGTCCTCGT CGGTGAACAG 
TGGCCTCTCC AAGCCCTTCT C

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wmc148(11g1) Plate reference : 11g1
WMC locus number : WMC148 Length of sequence : 226
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.7.SEQ. Trimmed to Rsa 1 at 5' end, and to insert at 3' end. Identical to 11g2.(CT)15 86 to 115. (3 loci - 1 ploymorphic but weak). 3' side of microsatellite matches 5g11. Bases 73 to 115 show homology to 25e10 bases 65 to 107 (complement) (43 in longest run, maxScore = 35).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
ccgATgAAgcgTgAgTAgTTTA at 23 length 22 bases, Tm 61
Reverse primer details :
gATcTcAAcAAAcAcAcTgccA at 174 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 173 bases.



Length of sequence : 226  Consensus sequence is :

..

GTACATCAAC CCTGTTGCCC TTCCGATGAA GCGTGAGTAG TTTACCACAG 
ACCTACGGGT CCAGAGCTAG AAGCTAGATG GCTTCCTCTC TCTCTCTCTC 
TCTCTCTCTC TCTCTGATTC TCAATACAAT GTTCTCCTCG ATGTTCTTGG 
AGATCTATTG ATGTAATCTT CTTTGGCAGT GTGTTTGTTG AGATCTGATG 
AATTGTGGAT TTATGATCAG CTTATC

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wmc149(11h1) Plate reference : 11h1
WMC locus number : WMC149 Length of sequence : 245
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
PIC Value : 0.83
Comments : AGR204.8.SEQ. Trimmed to Rsa1 at both ends. (CT)8 50 to 65, (CT)24 68 to 115. (3-4 loci, 2-3 polymorphic).
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
AcAgAcTTggTTggTgccgAgc at 6 length 22 bases, Tm 70
Reverse primer details :
ATgggcgggggTgTAgAgTTTg at 214 length 22 bases, Tm 70
Expected size of amplified fragment (Chinese Spring=) 230 bases.



Length of sequence : 245  Consensus sequence is :

..

GTACCACAGA CTTGGTTGGT GCCGAGCCCT TGCTCTAGCT CGCCGCGGTC 
TCTCTCTCTC TCTCTCCCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC 
TCTCTCTCTC TCTCTCGCCA CAAACGGAAG AAGAAAGGAG GAGAGAGCAC 
TGTGGACACA GCGAGGTTTT CCGGCGCACT AACACCTGCC GCAACAACGA 
CTTTTCCAGA GCACAAACTC TACACCCCCG CCCATTGCAG CGTAC

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wmc(11a2) Plate reference : 11a2
WMC locus number : WMC Length of sequence : 128
Last updated : Mer 14 juin 2000 at 10:34
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.9.SEQ. Trimmed to insert at both ends. (CT)13 44 to 69. No room for primers. Bases 16 to 54 show homology to 20a3 bases 1 to 39 (39 in longest run, maxScore = 27). Bases 1 to 36 show homology to TW1h07 bases 95 to 130 (complement) (36 in longest run, maxScore = 41). TW1h07 is a duplicate of 11a2, 11a2 has priority.
PCR Comments :



Length of sequence : 128  Consensus sequence is :

..

CACGCATCCA ACACACAAAC CTTGTTGGTC CCTCTCCCTC CATCTCTCTC 
TCTCTCTCTC TCTCTCTCTG TCTGTCTCTG TCTCTCTCTC AATCTCTCTC 
TCTCTCTGAC AATCTTCAGT TCTGTCGC

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wmc(11b2) Plate reference : 11b2
WMC locus number : WMC Length of sequence : 331
Last updated : Lun 5 juil 1999 at 17:23
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.10.SEQ. Trimmed to insert at both ends. (CA)9 43 to 60, (CA)25 242 to 29. Complex internal repeats, too difficult to design primers. Useful minisatellite probe? Related to 2H10 Bases 64 to 114 show homology to 12a12.2 bases 65 to 115 (complement) (51 in longest run, maxScore = 69). Bases 63 to 153 show homology to 26c10 bases 357 to 447 (91 in longest run, maxScore = 92).
PCR Comments :



Length of sequence : 331  Consensus sequence is :

..

GCGAGTCGAT GGTCAACTTG CAACTGGGGG CCACAAATAA AACACACACA 
CACACACACA TCCCTAGTTG CGAGTCGATG GGCAACTTGC AACTAGGGGT 
CCTCAACAAA CACACACACC CCCTAGTTAC AAGTCGGTGG TTGAGATGCA 
ACTCGGGGCC CCTTGAGAAA AAGACACACA CACACACATC CCCGTAGTTG 
CGAGCTGATG GCCAACTTGC AGCTGGGGGC CACGAATAAA ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACCCTCCTAG 
TTGCGAGTCG ATGGTCAACT TGCAACTGGG G

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wmc(11c2) Plate reference : 11c2
WMC locus number : WMC Length of sequence : 335
Last updated : Tue, Feb 25, 1997 at 22:30
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.11.SEQ. Trimmed to Rsa1 at 5' end, and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 335  Consensus sequence is :

..

GTACAGAGGG TGCGAGGTAG TTGACGCGGC CATGTGGCTA GTGGTGCTGG 
CAGCGAGGAT CGCGACAAGC TGGGGCGGGA TGAAGGGCGA CGCCGCAGGC 
GCGGTTGCAG ACAGGGTAGC GGAAGAGGAC GAGGTGGCGG CAGAGGAGCT 
CATGGCGACG GCGATCTTGG TGGCGGCGCG GCAACAAAGA AGTGCGGCGG 
CGGAAACCAA GGACAGGGAG CGGCGGCTAG GGTTTTGCGA AACCTAGTCT 
GATACCATGC TAAACAATGA CTTGGTGGAA AGTGGCTCAA CGCATCCGGT 
GCGTGGCCTA TCACTTTATA TAAGGTTAGT CCACG

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wmc(11d2) Plate reference : 11d2
WMC locus number : WMC Length of sequence : 183
Last updated : Tue, Feb 25, 1997 at 22:31
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.12.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 183  Consensus sequence is :

..

GGCCACCTGG TCGGGGCGGT TCTTCCCTCG CGATCTCCAC GTGCGTTCTA 
TCTATCCACT GCGACCGCCG CCGCCGCCGC CACCGATCCA CGTCTTGCCG 
CCGGTCACGT TACGCCATGG TTTCGTGGAG CGATGAGTCG AGCTCTTCTT 
CGGATGGCGG ATCCGTGACT AGCCTGCAGG TAC

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wmc(11e2) Plate reference : 11e2
WMC locus number : WMC Length of sequence : 374
Last updated : Tue, Feb 25, 1997 at 22:32
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.13.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. Identical to 11e1. No substantial microsatellite.
PCR Comments :



Length of sequence : 374  Consensus sequence is :

..

CATGAGATTC TGGTGGTTTG TGCATTATGC TTCTACATGT GGAGCTTTCT 
GGATTCTAAC ATGTGGCAAA ATCTGGTGGA ATGTAAGGTT AGGCAAGTTT 
GATTAACTTA GGTGAGTGTT TGGTTCAAGC CACACCTTAG GCAAGCCACA 
CTAAGGCCCC ACATGACATA CACACACAAA ATGTGGCAAG ATTCCCTTAG 
GCTTGCCAAC TTGTGGCTCT CATTTTGAAG AACTAATAAC TTTGCCTAAG 
CTTAGTTGTG GCAAAGTTAG TCATGAATTA AGTTCAACCA TGGAGTCTTC 
TAGATTATAC ATGTGGCAGA ATCTGGTGGA TGATATCCAA CGGCCAGTAG 
TGCTCGGATT TGCCATCTTT GTAC

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wmc(11f2) Plate reference : 11f2
WMC locus number : WMC Length of sequence : 270
Last updated : Tue, Feb 25, 1997 at 22:33
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.14.SEQ. Trimmed to Rsa1 at 5' end, and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 270  Consensus sequence is :

..

GTACTCGTAG GGCTGAGCGG GCGGCGGCGG CGGCATACGC ATCGGATGGA 
CCATTTGTCG ACAACGATCG ATGATCTGGT TGGAGTCTTG GAGATGACGC 
ACGATGGTGG GTTTGGTGCG ATCGACTCTC TTGATCGCTA GGTTTTGTAT 
AGGCTAGTTT TTTTCCTTTT GTGTGAAGAG TTTTGTAGGC TAGTTTTATT 
TCCTTTTCTA AGCGAGCGGG CCGGGGGTGT TATGTTTGAG CCCATCTCAT 
GTCAACGAGG GGAACCCCTG

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wmc(11g2) Plate reference : 11g2
WMC locus number : WMC Length of sequence : 226
Last updated : Thu, Feb 27, 1997 at 1:33 pm
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.15.SEQ. Trimmed to Rsa1 at 5' end and to insert at 3' end. Identical to 11g1. (CT)15 86 to 115.
PCR Comments :



Length of sequence : 226  Consensus sequence is :

..

GTACATCAAC CCTGTTGCCC TTCCGATGAA GCGTGAGTAG TTTACCACAG 
ACCTACGGGT CCAGAGCTAG AAGCTAGATG GCTTCCTCTC TCTCTCTCTC 
TCTCTCTCTC TCTCTGATTC TCAATACAAT GTTCTCCTCG ATGTTCTTGG 
AGATCTATTG ATGTAATCTT CTTTGGCAGT GTGTTTGTTG AGATCTGATG 
AATTGTGGAT TTATGATCAG CTTATC

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wmc150(11h2) Plate reference : 11h2
WMC locus number : WMC150 Length of sequence : 533
Last updated : Mar 10 Nov 1998 at 17:34
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.16.SEQ No overlap between forward and reverse sequences. Trimmed to insert at both ends. (GT)21 472 to 513. (5 loci, 3 polymorphic?). Complment of forward sequence matches 2c8, 6a6, 6b6, 7a2. Bases 90 to 110 show homology to 12a9 bases 461 to 481 (complement) (21 in longest run, maxScore = 113). Bases 225 to 285 show homology to 22h9 bases 71 to 131 (61 in longest run, maxScore = 92). Bases 123 to 287 show homology to 24d1 bases 7 to 171 (complement) (165 in longest run, maxScore = 171), duplicate, 11h2 has priority.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
cATTgATTgAAcAgTTgAAgAA at 358 length 22 bases, Tm 57
Reverse primer details :
cTcAAAgcAAcAgAAAAgTAAA at 501 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 165 bases.



Length of sequence : 533  Consensus sequence is :

..

AGAGGGGAAG GATCTCATGG GTTGAGGGAT TGACAAATTT GTGCGGGGGA 
GAGAGGGATT GGTAATTTTG TGCAAGAGAG AGAGAGGGTG GGGGGAGGAG 
TCAGTCAGCC ATCGTCACAT CACCCTGCTT CCAAATGACC CCGCATTCTC 
TAGCGCGGTG TGGTCGCCGT CTTTGATCTG CTCGATGCCC TCTTTGAAGA 
TTGAGGTGTT GTGCATGTTG GGGTGCAAAG AAGCACAAGC GAGAGTGGTC 
GCCGTATAAC CTTGGATGGG GATGAATTGT GTGCTCGAGG GAGTGTGTG
(break)
ATTT CAGCAAGAAT CAATAGCTTG TGTGATCCTT ATGTCGCTGA 
GTTCTTTTTA TAAACGTTCC ATTGATTGAA CAGTTGAAGA AGTATCATAT 
GTAGGTAAAC TGGTTAGACC TTTTAAAGTC TGCTGCTCTC ACAAGTATAT 
TTTGCATTAT TTTCCCTCCC TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTTTACTTT TCTGTTGCTT TGAGTATTAG

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wmc(11a3) Plate reference : 11a3
WMC locus number : WMC Length of sequence : 163
Last updated : Tue, Feb 25, 1997 at 22:36
Owner : Rogers, Monsanto Date at Agrogene : 21.2.97 Public Domain : Yes
Comments : AGR204.17.SEQ. Trimmed to insert at both ends. matched to 6e12, 7a3 (3rd insert), 5a10, 9g4. No substantial microsatellite. Bases 1 to 163 show homology to 12c3 bases 1 to 163 (163 in longest run, maxScore = 137).
PCR Comments :



Length of sequence : 163  Consensus sequence is :

..

ATATGCATTG CTGATGCTCA GTAAAAACTT ATATAGTTGA CACAATCATA 
TAGAACACCA TAGCACACTG AACTTGCATA CAAAAATTTC ACGCAGGCGG 
AACAATCAGG ATGGAAGCAG TGGATCGCTA CGGGTAGGGC TATGTTGAAA 
CCAACGAACT CGT

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