Fig. 4. Calcium- and palmitate-induced swelling in mitochondria isolated from cold-hardened seedlings of winter wheat (7 days at 4 C) under de-energized conditions. Experiments were at 26 C in an isotonic KCl-based swelling buffer, pH 7.4. Results are represented as the mean of at least three determinations per experiment. Traces: a and g - mitochondria without any addition; b - mitochondria after preincubation with CsA; c - mitochondria after preincubation with Ca+2 ions; d - mitochondria after preincubation with CsA followed by the addition of Ca+2 ions; e - mitochondria after addition of palmitic acid; f - mitochondria after preincubation with CsA followed by the addition of palmitic acid. Results are represented as the mean of at least three determinations per experiment.