Callus information and regeneration of plants from mature embryos in barley has been attempted using twenty six-rowed hulled varieties. Mature embryos were dissected from dehusked seeds and sterilized by immersion for 1 minute in 95% of ethanol and for 10 minutes in unfiltered 8% calcium hypochlorite solution, followed by five washings in sterilized distilled water . The culture medium employed was Gamborg's B5 medium supplemented with 0.03% yeast extract and with different concentrations (5, 10, 15, 30, and 100 M) of 2,4-D. All the cultures were kept in the light at 25°C for 2 months without transferring onto fresh medium. A variety Golden Promise, which is known as a good material for getting regenerants from calli derived from young embryos, showed regeneration of shoots also when calli were induced from mature embryo. A concentration of 10 M was found to be best (Table 1). No shoot regeneration was recognized in the other varieties. Transferring of the regenerants to a medium lacking, 2,4-D resulted in root formation. Callus cultures with transferring onto fresh medium supplemented with 0.03% yeast extracts and 5 M of 2,4-D once or twice per week for the first four weeks of culture were effective for induction of shoots from callus.