Multiple Dominant and Recessive Genetic Marker Stock Development

Multiple Dominant and Recessive Genetic Marker Stock Development
Robert I. Wolfeš, Patrick M. Hayes˛, and Les Shugarł
AAFC, Field Crop Development Centre, Lacombe AB Canada T4L 1W81,
Dept. of Crop and Soil Science, Oregon State Univ., Corvalis, OR 97331 USA2,
& W.G. Thompson & Sons Ltd., Ailsa Craig ON Canada N0M 1A03

Over a number of years the senior author has developed a set of multiple recessive and dominant genetic marker stocks. The primary material consists of a master recessive line, a master dominant, and several other stocks based in part on the master recessive, carrying recessive alleles specific for each of the seven chromosomes of barley. There are seven genetic male steriles, seven dwarfs, and five surface wax mutants. The recessive alleles of a few extra genes have been incorporated into these. See Table 1 for a list of contrasting alleles between the master recessive and master dominant, and Table 2 for a list of available combinations in the chromosome specific stocks.

Over the last few years we have been producing doubled haploids from a cross between the master recessive and master dominant. Prior to their production the two parents were also doubled. About 110 doubled haploid progeny are presently under increase.

Table 1. Major scorable Alleles in Master Recessive and Master Dominant Stock

						
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Chr.    Gene desig.     Brief description       
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1       N n             N Hulled; n Hulless             
1       Wx wx           Wx  Blue stain, wx  Purple stain, 
                        of starch with dilute iodine/KI solution      
1?      Lk2 lk2 ?       Lk2 Normal awn and hood; lk2 ? 
                        Short awn  (hood size??)         
2       Re2 re2         Re2 Purple lemma and pericarp. 
                        In Mdom stock; visible only just prior to 
                        when the black pigment of 'B' develops         
2       V v             VTwo-row (deficiens), vSix-row                
2       Wst,,c wst,,c   Wst,,c  Normal, wst,,k  White stripes on 
                        seedling leaves                
2       Zeo zeo         Zeo Semi-dominant dwarf with very dense 
                        head, zeo Normal                
2?      Ea? ea          Ea? Early heading (In both stocks)              
3       Al al           al Albino lemma and nodes               
3       Pub pub         Pub Pubescence on upper leaf blades             
4       Hs hs           Hs Hairs on lower leaf sheaths          
4       K k             K Hood, k Awn           
5       Cer-yy cer-yy   Cer-yy Dominant shiny head 
                        (Probably in both stocks)            
5       B b             B Black (Strong) seed, lemma & palea            
6       O o             o Orange lemma & nodes          
7       S s             S Long hairs on rachilla, s Short               
7       R r             R Rough awn, r smooth awn (At base)             
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Table 2. Summary of Multiple Recessive Stocks currently available for specific Chromosomes

     
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Chromosome  Genet. m. Ster.  Dwarf    Surf. Wax Mut.  Other               
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Chros 1     msg10            br       Cer-a-(Gs3)     wx
            msg10            br       cer-a(gs3)      wx      
Chros 2     msg2             mnd,,cšgs6             e li            
            Msg2             Mnd,,c   gs6             e li            
            msg2             Mnd,,c   gs6             e li            
            Msg2             Mnd,,c   gs6             e li            
Chros 3     msg5             uz        -              Als             
            Msg5             uz        -              als     
Chros 4     msg24            ert-i    gl              yh
            Msg24            ert-i    gl              yh      
Chros 5     msg              ert-b˛   cer-a           trd˛ nec,,_            
            Msg              ert-b    cer-a           trd nec,,_      
Chros 6     msg36            l9       gs4             - 
            Msg36            l9       gs4             -
            Msg36            l9       -               uc2
Chros 7     msg19            ert-g    -               -
            Msg19            ert-g    -               -
            msg19            ert-g    -               csł             
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šThe many noded gene (mnd,,c) is probably not on chromosome 2. Dr. J. Franckowiak did not find any linkage with chros 2 markers when backcrossing chromosome 2 genes of these stocks into Bowman. Many noded (mnd,,c mnd,,c) plants have been hard to grow in the greenhouse, and in the field are several days later heading and have smaller heads than their normal counterparts.
˛The ert-b trd combination produces plants that are slow to head and suffer from head encasement in the flag leaf sheath.
ł'corn stalk' (cs) produces variable tiller necrosis. Occasionally a large single tillered plant results.