II. 43 Evidence for assigning the high amylose locus, amo1 of Glacier barley to chromosome 3.
S.E. Ullrich and R.F. Eslick. Department of Plant and Soil Science, Montana State University, Bozeman, Montana 59717.
Supported in part by USA AID/ta-C-1094.
The high amylose trait (40% amylose) of Glacier barley first described by Merritt (1967) was found to be inherited as a single recessive gene and to display dosage effects, but no maternal effects, (Walker and Merritt, 1969). Eslick and Ullrich (1977) have suggested the gene be designated amo1 and have demonstrated that low kernel weight and high lysine are associated traits.
High Amylose Glacier was crossed with a series of Betzes trisomics to assign the amo1 locus to a chromosome (see Tsuchiya, 1964). Evidence for the location of amo1 on chromosome 2 is presented in Table 1. Additionally, the occurrence of homozygous recessive F3 rows from F2 trisomics from crosses with trisomics 3, 4 and 7 eliminates chromosomes 3, 4 and 7 from consideration. Trisomic 5 cross data are lacking, but Walker (1969) has indicated failure to detect linkage between amo1 and marker genes on chromosomes 4, 5 and 7. Linkage data from a relatively small population for amo1 and V indicated that the two traits were independent. Ramage and Eslick (1975) assigned the V locus approximately 24 recombination units from the centromere and msg2 on the long arm of chromosome 2. Additional information is needed to specifically locate amo1 on chromosome 2.
Table 1. F2 segregation from Betzes trisomic x High Amylose Glacier (amo1) crosses.
References:
Eslick, R.F. and S.E. Ullrich. 1977. A characterization of three high lysine mutants in relation to their normal isotypes and their environmental response. Barley Newsletter 20:39-45.
Merritt, N.R. 1967. A new strain of barley with starch of high amylose content. J. Inst. Brew. (London) 73:583-585.
Ramage, R.T. and R.F. Eslick. 1975. Translocation linkage tests T2-7a x male sterile genes. BGN 5:46-48.
Tsuchiya, T. 1964. Chromosome abberations and their use in genetics and breeding in barley - trisomics and anneploids. pp. 116-150. In Barley Genetics I. Proc. 1st Intl. Barley Genet. Symp. Centre for Agric. Publ. and Doc., Wageningen, Netherlands.
Walker, J.T. 1969. The high amylose locus in Glacier barley. Submitted at 2nd Intl. Barley Genet. Symp., Pullman, Washington, Unpublished.
Walker, J.T. and N.R. Merritt. 1969. Genetic control of abnormal starch
granules and high amylose content in a mutant of Glacier
barley. Nature (London) 221:482-483.
