From David Laurie, 7/94
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Reference: DA Laurie, N Pratchett, KM Devos, IJ Leitch & MD Gale
The distribution of RFLP markers on chromosome 2(2H) of barley in
relation to the physical and genetic location of 5S rDNA.
Theor. Appl. Genet. 87: 177-183 (1993)
This paper gives maps of barley chromosome 2 from two crosses. Eleven
probes were mapped in both crosses, so the maps can be aligned reasonably
well. This paper shows the map in relation to the physical position of
the 5S genes which was determined using in situ hybridization by Leitch and
Heslop-Harrison Genome 36: 517-523 (1993). We also used their nomenclature
for the sites.
Cross 1: Betzes x Golden Promise
120 F2 plants
Locus name cM distance (Kosambi mapping function)
Xpsb31a
0.4
Xpsb31b
6.0
Xpsr566
3.0
Xpsr150
0
XMWG878
0.4
XBCD175
10.3
Xpsr109
1.7
Xpsr666
7.2
Xpsr108
9.6
Xpsb9
39.5 (this interval includes the centromere)
Xpsr102
0
XCDO366
0
XCDO588
20.8
X5SDna-H3
3.4
Xpsr687
3.4
Xpsr901
1.5
Xpsr630
22.9
Xpsr540
0
XCDO373
6.1
XBCD266
30.4
XWG645
Cross 2: Captain x Hordeum spontaneum
120 F2 plants
Locus name cM distance (Kosambi mapping function)
Xpsb31
3.1
Xpsr566
3.7
Xpsr150
12.7
Xpsr109
5.2
XMWG858
3.8
Xpsb9
20.0
Xpsb24
20.1
Xpsb30
0.6
XSs2
2.1
Xpsr126
5.5 (this interval includes the centromere)
XWG996
9.3
Xpsr102
0
XCDO588
18.0
XMWG865
2.7
X5SDna-H3
2.7
Xpsb141
10.0
Xpsr901
0.4
Xpsr630
8.8
Xpsr331
22.5
XBCD266
26.9
XBCD410
3.1
XWG645
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Reference: DA Laurie, N Pratchett, C Romero, E Simpson & JW Snape
Assignment of the denso dwarfing gene to the long arm of chromosome 3(3H)
of barley by use of RFLP markers.
Plant Breeding 111: 198-203 (1993).
Cross: Magnum x Goldmarker
113 doubled haploid lines (derived by the Hordeum bulbosum system).
Locus name cM distance (Kosambi mapping function)
XWG940
0.0
Xpsr78
23.8
Xpsr74
0.0
Xpsb96
4.0
Xpsr170
7.8
denso
44.8
Xpsb177
All these markers are on the long arm.
The denso dwarfing gene has been widely used in European barley breeding.
It confers a prostrate juvenile growth habit,
later ear emergence time (heading date)
and reduced plant height.
It also gives smaller grains and tends to be associated with lower
malting quality, though many malting varieties have the gene.
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Reference: N Pratchett & DA Laurie
Genetic map location of the barley developmental mutant liguleless
in relation to RFLP markers.
Hereditas 120: 35-39 (1994)
This paper used a bulk segregant analysis to locate the barley
li mutation.
Cross : Tayeh-13 (a Japanese variety carrying liguleless) x Betzes
250 F2 plants from which 36 with the liguleless phenotype were
characterized with markers.
Locus name cM distance (Kosambi mapping function)
XCDO366
16.4
hex-v
11.4
Xpsr901
3.5
Xpsr331
16.5
XCDO373
4.1
XBCD266
1.3
liguleless
23.9
XBCD410
5.7
XWG645
All these markers are on the long arm.
hex-v controls the 2-row/6-row ear type. It cosegregates with XMWG865
- see other group 2 maps (Andreas Graner pers. comm).
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Reference: DA Laurie, N Pratchett, JH Bezant and JW Snape.
Genetic analysis of a photoperiod response gene on the short arm of
chromosome 2(2H) of barley (Hordeum vulgare).
Heredity 72:619-627 (1994)
A photoperiod response gene (designated Ppd-H1) was mapped. It affects
flowering time under long days (13h or 16h light) but has little
effect under short days (10h). In the field it is the major determinant
of flowering time variation in the doubled haploid population.
Its map location suggests it may be equivalent to the Ppd series of
photoperiod response genes in wheat.
This cross also has a second gene (a QTL) affecting flowering time
on this chromosome. However, this gene seems to affect heading data
pretty well independently of day length. The second gene is probably
in the Xpsb24-Xpsr571 interval
Cross 1: Igri x Triumph
94 doubled haploid lines (derived by the Hordeum bulbosum method).
Locus name cM distance (Kosambi mapping function)
XMWG878
17.7
Xpsr666
2.1
Xpsr108
1.7
XMWG858
1.0
Ppd-H1
5.1
Xpsb9
20.9
Xpsb24
21.1 (the centromere is in this interval)
Xpsr571
31.4
XMWG865
26.5
Xpsr87
0.0
Xpsr331
26.6
XBCD266
28.1
XBCD410
Final note:
All the map distances are from MAPMAKER.
psr markers are from the Cambridge Lab wheat RFLP group.
psb markers are our own barley clones. They are PstI genomic
fragments cloned in pUC18. (In the first papers they were called
psrB markers, but Mike Gale has since persuaded me to change to a
three letter symbol).
BCD, CDO and WG probes were mapped in barley by Manfred Heun et al.
(Genome 34: 437-447) and were kindly sent to me by Mark Sorrells.
MWG probes are from Andreas Graner. Most are on his map - Graner
et al. Theor. Appl. Genet. 83: 250-256 (1991).
The common markers allow the maps to be aligned with our wheat and rye
maps and with the barley maps from Cornell, NABGMP and Germany.