CONSTRUCTION OF PLANT BACTERIAL ARTIFICIAL CHROMOSOME (BAC) LIBRARIES: AN ILLUSTRATED GUIDE
INTRODUCTION
CHAPTER 1 Overview
CHAPTER 2 Supplies, equipment, & reagents
VECTOR PREPARATION
CHAPTER 3 Vector isolation
CHAPTER 4 Test ligation
CHAPTER 5 Test transformation
CHAPTER 6 Miniprep & NotI digest
PREPARATION OF INSERT DNA
CHAPTER 7 Isolation of high molecular weight nuclear DNA
CHAPTER 8 DNA analysis
CHAPTER 9 Test restriction digest
CHAPTER 10 Restriction digest
CHAPTER 11 First size selection
CHAPTER 12 Second size selection
CHAPTER 13 Isolation of size-selected DNA from agarose
LIBRARY CONSTRUCTION
CHAPTER 14 Ligation, test transformation, & NotI digest
CHAPTER 15 Mass Transformation
CHAPTER 16 Picking clones
CHAPTER 17 Library replication & storage
APPENDICES
APPENDIX A Using the Gibco BRL Cell-Porator System
APPENDIX B Using the Bio-Rad Model 422 Electroelution System
APPENDIX C Filling 384-well plates using the Genetix QFill2
APPENDIX D The Genetix QBot
APPENDIX E Picking clones using the Genetix QBot
APPENDIX F Library replication using the Genetix QBot